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Int J Fertil Steril. 2012 Jul;6(2):111-6. Epub 2012 Jun 19.

Nitric oxide-induced polycystic ovaries in the wistar rat.

Author information

1
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.
2
Department of Pathology, School of Medicine, Shahed University, Tehran, Iran.
3
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Abstract

BACKGROUND:

Nitric oxide (NO) involves in polycystic ovary syndrome (PCOS), a cause of infertility in women during the reproductive age. The PCOS is now categorized as an inflammatory phenomenon. The aim of this study was to evaluate the role of NO, a proinflammatory agent, in this syndrome at histological and biochemical levels.

MATERIALS AND METHODS:

In this experimental study, animals were female Wistar rats (weighing 200-250 g) kept under standard conditions. L-Arginine (50-200 mg/kg), a precursor of NO, was injected intra-peritoneally (i.p.) through a period ranging from 9 to14 days/ once a day. The rats' estrous cycle was studied using Papanicolaou test; those showing phase of Diestrous were grouped into experimental and control groups. The control group solely received saline (1 ml/kg, i.p.) throughout all experiments. To evaluate the inflammatory effect of NO, the rats were treated an anti-inflammatory agent, naloxone hydrochloride (0.4 mg/kg, i.p.), prior to L-arginine. At the end of the treatment period all animals' ovaries were assessed for histopathological and histochemical investigations. Also, activation of NO synthase (NOS) in the experiments was studied using NADPH-diaphorase technique.

RESULTS:

The ovaries of rats treated with L-arginine showed polycystic characteristics in contrast to those collected from control or naloxone pretreated groups, based on image analysis. A difference in enzyme activation was also shown in the sections that belonged to the groups that received L-arginine when compared with the pre-naloxone and control groups.

CONCLUSION:

Based on these results, we believe that NO may play a major role in the pathophysiology of PCOS.

KEYWORDS:

L-Arginine; NADPH-Diaphorase; Naloxone; Nitric oxide; PCOS

PMID:
25493168
PMCID:
PMC4258239

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