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Exp Toxicol Pathol. 2017 Jun 14;69(5):285-291. doi: 10.1016/j.etp.2017.01.014. Epub 2017 Feb 9.

Pathological and biochemical evaluation of coumarin and chlorophyllin against aflatoxicosis in rat.

Author information

1
Biochemistry Department, Faculty of Agriculture, Cairo University, Giza, Egypt.
2
Regional Center For Food and Feed, Agriculture Research Center, Ministry of Agriculture, Giza, Egypt.
3
Pathology Department, Faculty of Veterinary Medicine, Cairo University, Giza Square, Giza, 12211, Egypt. Electronic address: Marwakhattab@cu.edu.eg.

Abstract

Aflatoxin contamination of animal diet has adverse effects on animal health and productivity. This study was performed to investigate the effect of using coumarin and/or chlorophyllin in rat diet against aflatoxicosis. Fifty-four rats were assigned into 7 groups (6 rats each). G1 was a negative control. G2 received water with coumarin 0.5%. G3 received water with chlorophyllin 0.5%. G4 received water with coumarin 0.5% and chlorophyllin 0.5%. G5-8 fed aflatoxin B1 1000ppb in diet. Group 6-8 were administered similar treatments as G2-4. The experiment ended after 8 weeks. Random glucose, total lipid, total cholesterol, total triglycerides, total protein, serum ALT, AST, creatinine, and urea were measured. Histopathology of liver, kidney and pancreas and immunohistochemical staining of placental glutathione-S-transferase (GST-P) in liver were performed. The glucose serum level, cholesterol, AST, and ALT were elevated in G5 compared to G6-8. The liver and kidney lesions in G5 included vacuolation and necrosis which subsided in G6-8. The necrosis and inflammatory cells infiltration in the pancreas of G5 were absent in G6-8. GST-P positive hepatocytes were abundant in G5, few in G6 and absent in G7 and G8. In conclusion, the chlorophyllin and coumarin possessed protective and anti-carcinogenic effect against aflatoxicosis in rats.

KEYWORDS:

Aflatoxin; Chlorophyllin; Coumarin; Immunohistochemistry; Placental glutathione-S-transferase

PMID:
28190563
DOI:
10.1016/j.etp.2017.01.014
[Indexed for MEDLINE]

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