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Evolving a lipase for hydrolysis of natural triglycerides along with enhanced tolerance towards a protease and surfactants.

Alfaro-Chávez AL, Liu JW, Stevenson BJ, Goldman A, Ollis DL.

Protein Eng Des Sel. 2019 Sep 3. pii: gzz023. doi: 10.1093/protein/gzz023. [Epub ahead of print]


Improving on nature's shortcomings: evolving a lipase for increased lipolytic activity, expression and thermostability.

Alfaro-Chávez AL, Liu JW, Porter JL, Goldman A, Ollis DL.

Protein Eng Des Sel. 2019 Sep 10;32(1):13-24. doi: 10.1093/protein/gzz024.


Structure of the UspA1 protein fragment from Moraxella catarrhalis responsible for C3d binding.

Mikula KM, Kolodziejczyk R, Goldman A.

J Struct Biol. 2019 Nov 1;208(2):77-85. doi: 10.1016/j.jsb.2019.08.002. Epub 2019 Aug 7.


Glutamate transporters: a broad review of the most recent archaeal and human structures.

Pavić A, Holmes AOM, Postis VLG, Goldman A.

Biochem Soc Trans. 2019 Aug 30;47(4):1197-1207. doi: 10.1042/BST20190316. Epub 2019 Aug 5. Review. Erratum in: Biochem Soc Trans. 2019 Sep 5;:.


Asymmetry in catalysis by Thermotoga maritima membrane-bound pyrophosphatase demonstrated by a nonphosphorus allosteric inhibitor.

Vidilaseris K, Kiriazis A, Turku A, Khattab A, Johansson NG, Leino TO, Kiuru PS, Boije Af Gennäs G, Meri S, Yli-Kauhaluoma J, Xhaard H, Goldman A.

Sci Adv. 2019 May 22;5(5):eaav7574. doi: 10.1126/sciadv.aav7574. eCollection 2019 May.


Roles of the Hydrophobic Gate and Exit Channel in Vigna radiata Pyrophosphatase Ion Translocation.

Tsai JY, Tang KZ, Li KM, Hsu BL, Chiang YW, Goldman A, Sun YJ.

J Mol Biol. 2019 Apr 5;431(8):1619-1632. doi: 10.1016/j.jmb.2019.03.009. Epub 2019 Mar 13.


Substrate polyspecificity and conformational relevance in ABC transporters: new insights from structural studies.

Wright J, Muench SP, Goldman A, Baker A.

Biochem Soc Trans. 2018 Dec 17;46(6):1475-1484. doi: 10.1042/BST20180146. Epub 2018 Dec 4. Review.


Defining Dynamics of Membrane-Bound Pyrophosphatases by Experimental and Computational Single-Molecule FRET.

Harborne SPD, Strauss J, Turku A, Watson MA, Tuma R, Harris SA, Goldman A.

Methods Enzymol. 2018;607:93-130. doi: 10.1016/bs.mie.2018.04.017.


A Simple Strategy to Determine the Dependence of Membrane-Bound Pyrophosphatases on K+ as a Cofactor.

Strauss J, Wilkinson C, Vidilaseris K, Harborne SPD, Goldman A.

Methods Enzymol. 2018;607:131-156. doi: 10.1016/bs.mie.2018.04.018. Epub 2018 Jun 1.


Binding of EphrinA5 to RET receptor tyrosine kinase: An in vitro study.

Liu Y, Kaljunen H, Pavić A, Saarenpää T, Himanen JP, Nikolov DB, Goldman A.

PLoS One. 2018 Jun 11;13(6):e0198291. doi: 10.1371/journal.pone.0198291. eCollection 2018.


Pacing across the membrane: the novel PACE family of efflux pumps is widespread in Gram-negative pathogens.

Hassan KA, Liu Q, Elbourne LDH, Ahmad I, Sharples D, Naidu V, Chan CL, Li L, Harborne SPD, Pokhrel A, Postis VLG, Goldman A, Henderson PJF, Paulsen IT.

Res Microbiol. 2018 Sep - Oct;169(7-8):450-454. doi: 10.1016/j.resmic.2018.01.001. Epub 2018 Feb 2. Review.


Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action.

Robinson JI, Baxter EW, Owen RL, Thomsen M, Tomlinson DC, Waterhouse MP, Win SJ, Nettleship JE, Tiede C, Foster RJ, Owens RJ, Fishwick CWG, Harris SA, Goldman A, McPherson MJ, Morgan AW.

Proc Natl Acad Sci U S A. 2018 Jan 2;115(1):E72-E81. doi: 10.1073/pnas.1707856115. Epub 2017 Dec 15.


Crystal structure of a tripartite complex between C3dg, C-terminal domains of factor H and OspE of Borrelia burgdorferi.

Kolodziejczyk R, Mikula KM, Kotila T, Postis VLG, Jokiranta TS, Goldman A, Meri T.

PLoS One. 2017 Nov 30;12(11):e0188127. doi: 10.1371/journal.pone.0188127. eCollection 2017.


Using a SMALP platform to determine a sub-nm single particle cryo-EM membrane protein structure.

Parmar M, Rawson S, Scarff CA, Goldman A, Dafforn TR, Muench SP, Postis VLG.

Biochim Biophys Acta Biomembr. 2018 Feb;1860(2):378-383. doi: 10.1016/j.bbamem.2017.10.005. Epub 2017 Oct 6.


Obesity: Receptors identified for a weight regulator.

Saarma M, Goldman A.

Nature. 2017 Oct 12;550(7675):195-197. doi: 10.1038/nature24143. Epub 2017 Sep 27. No abstract available.


Zebrafish GDNF and its co-receptor GFRα1 activate the human RET receptor and promote the survival of dopaminergic neurons in vitro.

Saarenpää T, Kogan K, Sidorova Y, Mahato AK, Tascón I, Kaljunen H, Yu L, Kallijärvi J, Jurvansuu J, Saarma M, Goldman A.

PLoS One. 2017 May 3;12(5):e0176166. doi: 10.1371/journal.pone.0176166. eCollection 2017.


Insights into the mechanism of membrane pyrophosphatases by combining experiment and computer simulation.

Shah NR, Wilkinson C, Harborne SP, Turku A, Li KM, Sun YJ, Harris S, Goldman A.

Struct Dyn. 2017 Mar 3;4(3):032105. doi: 10.1063/1.4978038. eCollection 2017 May.


Membrane pyrophosphatases from Thermotoga maritima and Vigna radiata suggest a conserved coupling mechanism.

Li KM, Wilkinson C, Kellosalo J, Tsai JY, Kajander T, Jeuken LJ, Sun YJ, Goldman A.

Nat Commun. 2016 Dec 6;7:13596. doi: 10.1038/ncomms13596.


Transport mechanism of a glutamate transporter homologue GltPh.

Ji Y, Postis VL, Wang Y, Bartlam M, Goldman A.

Biochem Soc Trans. 2016 Jun 15;44(3):898-904. doi: 10.1042/BST20160055. Review.


Artificial membranes for membrane protein purification, functionality and structure studies.

Parmar MJ, Lousa Cde M, Muench SP, Goldman A, Postis VL.

Biochem Soc Trans. 2016 Jun 15;44(3):877-82. doi: 10.1042/BST20160054. Review.

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