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Virus Res. 2019 Jan 2;259:77-84. doi: 10.1016/j.virusres.2018.10.019. Epub 2018 Oct 25.

Comparative analysis and characterization of Enterobacteria phage SSL-2009a and 'HK578likevirus' bacteriophages.

Li S1, Lu S2, Huang H3, Tan L4, Ni Q5, Shang W6, Yang Y7, Hu Z8, Zhu J9, Li M10, Rao X11, Hu F12, Hu X13.

Author information

1
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: 1416234121@qq.com.
2
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: shulang88@126.com.
3
Department of Clinical Laboratory Science, Army Medical University, Chongqing, China. Electronic address: huanghui@tmmu.edu.cn.
4
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: 540899224@qq.com.
5
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: qsni@tmmu.edu.cn.
6
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: shangwl0414@163.com.
7
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: yangyi19871230@126.com.
8
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: 35090772@qq.com.
9
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: zhujunmincn@126.com.
10
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: sotx7080@163.com.
11
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: raoxiancai@126.com.
12
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: hufuquan2012@aliyun.cn.
13
Department of Microbiology, Army Medical University, Chongqing, China. Electronic address: huxiaomei@tmmu.edu.cn.

Abstract

Enterobacteria phage SSL-2009a is a virulent bacteriophage with strong and abroad lytic ability against lots of engineering E. coli strains. In this study, we re-sequenced its whole genome and made a detail analysis on its genomic and proteomic characteristics according to the updated genomic sequence. The genome of SSL-2009a is a circular double-stranded DNA of 44,899 base pairs in length, with a 54.67% G + C content. A total of 67 open reading frames were predicted as protein coding sequences, 24 of which encode products highly homologous to known phage proteins. There are 10 promoters and 22 terminators identified in the genome of SSL-2009a, but no tRNA is found. SSL-2009a belongs to the 'HK578likevirus' genus of Siphoviridae. Comparative analyses indicated that other twelve phages share high homology with SSL-2009a at nucleotide and amino acid levels and also should be clustered into the same genus. In-depth analysis was performed to reveal the genomic, proteomic, and morphological features of these 'HK578likevirus' phages, which may promote our understanding of Enterobacteria phage SSL-2009a and the 'HK578likevirus' genus, even the biodiversity and evolution of bacteriophages.

KEYWORDS:

Bacteriophage; Comparative analysis; Enterobacteria phage SSL-2009a; Genome annotation; HK578likevirus

PMID:
30395896
DOI:
10.1016/j.virusres.2018.10.019
[Indexed for MEDLINE]
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