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Phytochemistry. 2000 Dec;55(7):853-7.

Caffeine and theobromine in epicuticular wax of Ilex paraguariensis A. St.-Hil.

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Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, RS, Brazil.


Caffeine and theobromine were identified and quantified in leaf epicuticular waxes of Ilex paraguariensis A. St.-Hil. (Aquifoliaceae). The total epicuticular leaf wax content was ca. 0.5% on average of dry leaf weight. Epicuticular caffeine and theobromine contents varied from 0.16 to 127.6 microg/mg and from 0 to 9.5 microg/mg of wax, respectively. For some selected samples, the intracellular methylxanthine concentration was also determined. A positive correlation was found between inner and epicuticular caffeine contents.

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