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Items: 1 to 20 of 244


The Soybean Lipoxygenase-Substrate Complex: Correlation between the Properties of Tunneling-Ready States and ENDOR-Detected Structures of Ground States.

Offenbacher AR, Sharma A, Doan PE, Klinman JP, Hoffman BM.

Biochemistry. 2020 Feb 25;59(7):901-910. doi: 10.1021/acs.biochem.9b00861. Epub 2020 Feb 5.


Electron Paramagnetic Resonance Spectroscopic Identification of the Fe-S Clusters in the SPASM Domain-Containing Radical SAM Enzyme PqqE.

Tao L, Zhu W, Klinman JP, Britt RD.

Biochemistry. 2019 Dec 24;58(51):5173-5187. doi: 10.1021/acs.biochem.9b00960. Epub 2019 Dec 11.


Comparative kinetic isotope effects on first- and second-order rate constants of soybean lipoxygenase variants uncover a substrate-binding network.

Hu S, Offenbacher AR, Lu ED, Klinman JP.

J Biol Chem. 2019 Nov 29;294(48):18069-18076. doi: 10.1074/jbc.RA119.010826. Epub 2019 Oct 17.


Detecting and Characterizing the Kinetic Activation of Thermal Networks in Proteins: Thermal Transfer from a Distal, Solvent-Exposed Loop to the Active Site in Soybean Lipoxygenase.

Zaragoza JPT, Nguy A, Minnetian N, Deng Z, Iavarone AT, Offenbacher AR, Klinman JP.

J Phys Chem B. 2019 Oct 17;123(41):8662-8674. doi: 10.1021/acs.jpcb.9b07228. Epub 2019 Oct 3.


A two-component protease in Methylorubrum extorquens with high activity toward the peptide precursor of the redox cofactor pyrroloquinoline quinone.

Martins AM, Latham JA, Martel PJ, Barr I, Iavarone AT, Klinman JP.

J Biol Chem. 2019 Oct 11;294(41):15025-15036. doi: 10.1074/jbc.RA119.009684. Epub 2019 Aug 19.


Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments.

Masson GR, Burke JE, Ahn NG, Anand GS, Borchers C, Brier S, Bou-Assaf GM, Engen JR, Englander SW, Faber J, Garlish R, Griffin PR, Gross ML, Guttman M, Hamuro Y, Heck AJR, Houde D, Iacob RE, Jørgensen TJD, Kaltashov IA, Klinman JP, Konermann L, Man P, Mayne L, Pascal BD, Reichmann D, Skehel M, Snijder J, Strutzenberg TS, Underbakke ES, Wagner C, Wales TE, Walters BT, Weis DD, Wilson DJ, Wintrode PL, Zhang Z, Zheng J, Schriemer DC, Rand KD.

Nat Methods. 2019 Jul;16(7):595-602. doi: 10.1038/s41592-019-0459-y. Epub 2019 Jun 27. Review.


Moving Through Barriers in Science and Life.

Klinman JP.

Annu Rev Biochem. 2019 Jun 20;88:1-24. doi: 10.1146/annurev-biochem-013118-111217.


Discovery of Hydroxylase Activity for PqqB Provides a Missing Link in the Pyrroloquinoline Quinone Biosynthetic Pathway.

Koehn EM, Latham JA, Armand T, Evans RL 3rd, Tu X, Wilmot CM, Iavarone AT, Klinman JP.

J Am Chem Soc. 2019 Mar 13;141(10):4398-4405. doi: 10.1021/jacs.8b13453. Epub 2019 Feb 27.


Biophysical Characterization of a Disabled Double Mutant of Soybean Lipoxygenase: The "Undoing" of Precise Substrate Positioning Relative to Metal Cofactor and an Identified Dynamical Network.

Hu S, Offenbacher AR, Thompson EM, Gee CL, Wilcoxen J, Carr CAM, Prigozhin DM, Yang V, Alber T, Britt RD, Fraser JS, Klinman JP.

J Am Chem Soc. 2019 Jan 30;141(4):1555-1567. doi: 10.1021/jacs.8b10992. Epub 2019 Jan 15.


Understanding Biological Hydrogen Transfer Through the Lens of Temperature Dependent Kinetic Isotope Effects.

Klinman JP, Offenbacher AR.

Acc Chem Res. 2018 Sep 18;51(9):1966-1974. doi: 10.1021/acs.accounts.8b00226. Epub 2018 Aug 28. Review.


Methods for Expression, Purification, and Characterization of PqqE, a Radical SAM Enzyme in the PQQ Biosynthetic Pathway.

Zhu W, Martins AM, Klinman JP.

Methods Enzymol. 2018;606:389-420. doi: 10.1016/bs.mie.2018.04.002.


X-ray and EPR Characterization of the Auxiliary Fe-S Clusters in the Radical SAM Enzyme PqqE.

Barr I, Stich TA, Gizzi AS, Grove TL, Bonanno JB, Latham JA, Chung T, Wilmot CM, Britt RD, Almo SC, Klinman JP.

Biochemistry. 2018 Feb 27;57(8):1306-1315. doi: 10.1021/acs.biochem.7b01097. Epub 2018 Feb 6.


Activity-Related Microsecond Dynamics Revealed by Temperature-Jump Förster Resonance Energy Transfer Measurements on Thermophilic Alcohol Dehydrogenase.

Vaughn MB, Zhang J, Spiro TG, Dyer RB, Klinman JP.

J Am Chem Soc. 2018 Jan 24;140(3):900-903. doi: 10.1021/jacs.7b12369. Epub 2018 Jan 11.


Enhanced Rigidification within a Double Mutant of Soybean Lipoxygenase Provides Experimental Support for Vibronically Nonadiabatic Proton-Coupled Electron Transfer Models.

Hu S, Soudackov AV, Hammes-Schiffer S, Klinman JP.

ACS Catal. 2017 May 5;7(5):3569-3574. doi: 10.1021/acscatal.7b00688. Epub 2017 Apr 20.


Origins of Enzyme Catalysis: Experimental Findings for C-H Activation, New Models, and Their Relevance to Prevailing Theoretical Constructs.

Klinman JP, Offenbacher AR, Hu S.

J Am Chem Soc. 2017 Dec 27;139(51):18409-18427. doi: 10.1021/jacs.7b08418. Epub 2017 Dec 15.


Hydrogen-deuterium exchange reveals long-range dynamical allostery in soybean lipoxygenase.

Offenbacher AR, Iavarone AT, Klinman JP.

J Biol Chem. 2018 Jan 26;293(4):1138-1148. doi: 10.1074/jbc.M117.817197. Epub 2017 Nov 30.


At the confluence of ribosomally synthesized peptide modification and radical S-adenosylmethionine (SAM) enzymology.

Latham JA, Barr I, Klinman JP.

J Biol Chem. 2017 Oct 6;292(40):16397-16405. doi: 10.1074/jbc.R117.797399. Epub 2017 Aug 22. Review.


Crystal structures reveal metal-binding plasticity at the metallo-β-lactamase active site of PqqB from Pseudomonas putida.

Tu X, Latham JA, Klema VJ, Evans RL 3rd, Li C, Klinman JP, Wilmot CM.

J Biol Inorg Chem. 2017 Oct;22(7):1089-1097. doi: 10.1007/s00775-017-1486-8. Epub 2017 Aug 19.


Hydrogen-Deuterium Exchange of Lipoxygenase Uncovers a Relationship between Distal, Solvent Exposed Protein Motions and the Thermal Activation Barrier for Catalytic Proton-Coupled Electron Tunneling.

Offenbacher AR, Hu S, Poss EM, Carr CAM, Scouras AD, Prigozhin DM, Iavarone AT, Palla A, Alber T, Fraser JS, Klinman JP.

ACS Cent Sci. 2017 Jun 28;3(6):570-579. doi: 10.1021/acscentsci.7b00142. Epub 2017 Jun 9.


Nuclear Magnetic Resonance Structure and Binding Studies of PqqD, a Chaperone Required in the Biosynthesis of the Bacterial Dehydrogenase Cofactor Pyrroloquinoline Quinone.

Evans RL 3rd, Latham JA, Xia Y, Klinman JP, Wilmot CM.

Biochemistry. 2017 May 30;56(21):2735-2746. doi: 10.1021/acs.biochem.7b00247. Epub 2017 May 12.

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