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Cell Rep. 2013 Oct 17;5(1):44-50. doi: 10.1016/j.celrep.2013.09.021. Epub 2013 Oct 10.

Using micromanipulation to analyze control of vertebrate meiotic spindle size.

Author information

1
Department of Physics, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.

Abstract

The polymerization/depolymerization dynamics of microtubules (MTs) have been reported to contribute to control of the size and shape of spindles, but quantitative analysis of how the size and shape correlate with the amount and density of MTs in the spindle remains incomplete. Here, we measured these parameters using 3D microscopy of meiotic spindles that self-organized in Xenopus egg extracts and presented a simple equation describing the relationship among these parameters. To examine the validity of the equation, we cut the spindle into two fragments along the pole-to-pole axis by micromanipulation techniques that rapidly decrease the amount of MTs. The spheroidal shape spontaneously recovered within 5 min, but the size of each fragment remained small. The equation we obtained quantitatively describes how the spindle size correlates with the amount of MTs while maintaining the shape and the MT density.

PMID:
24120869
PMCID:
PMC4155493
DOI:
10.1016/j.celrep.2013.09.021
[Indexed for MEDLINE]
Free PMC Article

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