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Mol Cell. 2020 Jan 2;77(1):17-25.e5. doi: 10.1016/j.molcel.2019.10.005. Epub 2019 Nov 5.

Tunability of DNA Polymerase Stability during Eukaryotic DNA Replication.

Author information

1
Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia; Illawarra Health & Medical Research Institute, Wollongong, NSW 2522, Australia.
2
Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA.
3
Laboratory of DNA Replication, Rockefeller University, New York, NY 10065, USA; Howard Hughes Medical Institute, Cambridge, MA 02138, USA.
4
Laboratory of DNA Replication, Rockefeller University, New York, NY 10065, USA; Howard Hughes Medical Institute, Cambridge, MA 02138, USA. Electronic address: odonnel@rockefeller.edu.
5
Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia; Illawarra Health & Medical Research Institute, Wollongong, NSW 2522, Australia. Electronic address: vanoijen@uow.edu.au.

Abstract

Structural and biochemical studies have revealed the basic principles of how the replisome duplicates genomic DNA, but little is known about its dynamics during DNA replication. We reconstitute the 34 proteins needed to form the S. cerevisiae replisome and show how changing local concentrations of the key DNA polymerases tunes the ability of the complex to efficiently recycle these proteins or to dynamically exchange them. Particularly, we demonstrate redundancy of the Pol α-primase DNA polymerase activity in replication and show that Pol α-primase and the lagging-strand Pol δ can be re-used within the replisome to support the synthesis of large numbers of Okazaki fragments. This unexpected malleability of the replisome might allow it to deal with barriers and resource challenges during replication of large genomes.

KEYWORDS:

DNA; DNA replication; dynamics; fluorescence; multi-protein complexes; polymerase; replication fork; replisome; single molecule; stability

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