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Anal Chim Acta. 2010 Apr 14;665(1):1-6. doi: 10.1016/j.aca.2010.03.014. Epub 2010 Mar 15.

Capillary electrophoresis with contactless conductivity detection coupled to a sequential injection analysis manifold for extended automated monitoring applications.

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University of Basel, Department of Chemistry, Spitalstrasse 51, 4056 Basel, Switzerland.


A capillary electrophoresis (CE) instrument with capacitively coupled contactless conductivity detection (C(4)D) based on a sequential injection analysis (SIA) manifold was refined. Hydrodynamic injection was implemented to avoid a sampling bias by using a split-injection device based on a needle valve for precise adjustment. For safety and reliability, the integrity of the high voltage compartment at the detection end was fully maintained by implementing flushing of the high voltage interface through the capillary. With this set-up, extended fully automated monitoring applications are possible. The system was successfully tested in the field for the determination of the concentration levels of major inorganic cations and anions in a creek over a period of 5 days.


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