Format

Send to

Choose Destination

Links from PubMed

See comment in PubMed Commons below
J Prev Med Hyg. 2012 Sep;53(3):165-8.

Adherence of Aeromonas hydrophila strains to human enterocyte-like cells pre-infected with rotavirus.

Author information

1
Department of Hygiene, Preventive Medicine and Public Health, University of Messina, Italy. mp.bertuccio@gmail.com

Abstract

INTRODUCTION:

The interest grown in these years about emerging pathogens in the onset of intestinal disease showed that the pathogenic mechanism is a multifactorial event. Our objective was to evaluate the role of co-infection with rotavirus in the expression of Aeromonas spp adhesiveness.

METHODS:

The rate of co-infection involves contact of Caco-2 cells with the virus, followed by adsorption for 1 and 2 hours. Aliquots of bacterial suspensions were added to tissue-culture plates. After infection, cell monolayers were lysed; serially diluted lysates were plated to determine the number of bound bacteria by performing colony forming units (CFU) counts.

RESULTS:

Non-adhesive strains were not subject to variations resulting from co-infection, while those who had medium or high adhesiveness gave rise to an increase of the same.

DISCUSSION:

Infection with rotavirus promotes the Aeromonas ability to adhere to Caco-2 cells and this effect depends on the duration of infection and on the starting adhesiveness of bacteria strain.

PMID:
23362623
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center