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Vaccine. 2010 Nov 16;28(49):7764-73. doi: 10.1016/j.vaccine.2010.09.054. Epub 2010 Sep 29.

Dendritic cells transfected with Her2 antigen-encoding RNA replicons cross-prime CD8 T cells and protect mice against tumor challenge.

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1
Institute of Biochemistry and Biotechnology, Faculty of Life Sciences (NFI), Section Microbial Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 3, D-06120 Halle/Saale, Germany.

Abstract

Antigen-specific T cells can be induced by direct priming and cross-priming. To investigate cross-priming as a vaccination approach dendritic cells were transfected with cytopathogenic viral RNA-replicons that expressed domains of the tumor-associated Her2-antigen and injected into MHC-discordant mice that did not allow direct priming. Upon tumor challenge 75% of the vaccinated, but none of the mock-vaccinated mice remained tumor-free. The anti-tumor effect required T cells and correlated with the vigor of the cross-primed CD8 T cell response. Her2-specific antibodies were not detected. This study highlights the potential of T cell cross-priming in cancer immunotherapy.

PMID:
20887827
PMCID:
PMC3399739
DOI:
10.1016/j.vaccine.2010.09.054
[Indexed for MEDLINE]
Free PMC Article
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