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Clin Biochem. 2008 Dec;41(18):1454-60. doi: 10.1016/j.clinbiochem.2008.08.074. Epub 2008 Sep 3.

Biomarkers of oxidative stress and endothelial dysfunction in glucose intolerance and diabetes mellitus.

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1
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.

Abstract

OBJECTIVES:

To evaluate biomarkers of endothelial dysfunction and oxidative stress in glucose intolerance (GI) compared to overt diabetes (DM2).

DESIGN AND METHODS:

140 volunteers including 96 with DM2, 32 with GI and 12 controls (C) were studied. (*)NO metabolites, (*)NO synthase inhibitors, thiols and N-acetyl-beta-glucosaminidase (NAGase) activity were analyzed by chemiluminescence, capillary electrophoresis, ELISA and colorimetric assay, respectively.

RESULTS:

(*)NO metabolites were higher in GI (NOx: p=0.03; S-nitrosothiols: p=0.001) and DM2 (p=0.006; p=0.0006) groups in relation to group C, while nitrotyrosine was higher only in the DM2 group in comparison to the other groups. NAGase activity was elevated in GI (p=0.003) and DM2 (p=0.0004) groups in relation to group C, as well as, ADMA (p=0.01; p=0.003) and GSSG (p=0.01; p=0.002).

CONCLUSIONS:

(*)NO metabolites, (*)NO synthase inhibitors, thiols and NAGase are biomarkers suitable to indicate endothelial dysfunction and oxidative stress in the early stages of impaired response to insulin.

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