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Med Sci Monit. 2004 Jun;10(6):BR180-4. Epub 2004 Jun 1.

PER-1 type beta-lactamase production in Acinetobacter baumannii is related to cell adhesion.

Author information

1
Dipartimento di Scienze biomediche, Sezione di Microbiologia sperimentale e clinica, University of Sassari, Viale San Pietro 43b, 07100 Sassari, Italy. sechila@uniss.it

Abstract

BACKGROUND:

Recently, the dissemination of ESBL (PER-1) among Acinetobacter isolates was reported in Turkish hospitals. We investigated the presence and the association of various virulence determinants in 20 Acinetobacter baumannii isolates, of which 13 were blaPER-1 positive.

MATERIAL/METHODS:

Virulence tests were slime and hemolysin production, gelatinase and protease activity, biofilm formation, and Caco2 cell adhesion. RAPD analysis was also performed with ERIC primers.

RESULTS:

None of the strains was positive for slime or hemolysin production and gelatinase or protease activity. A total of 16 strains, five of which were PER-1 gene negative, formed a biofilm on polymer surfaces. There was no relation between the presence of the PER gene and biofilm formation. On the other hand, nine strains (all PER-1 gene positive) were positive in adhesion experiments to Caco2 cell lines. All PER-negative strains were negative for cell adhesion.

CONCLUSIONS:

This study indicates the existence of a relation between PER-1 gene and cell adhesion in Acinetobacter strains.

PMID:
15173664
[Indexed for MEDLINE]

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