Format

Send to

Choose Destination

Links from PubMed

Methods. 2019 Jan 1;152:23-30. doi: 10.1016/j.ymeth.2018.08.006. Epub 2018 Aug 20.

Identifying and characterizing functional 3' nucleotide addition in the miRNA pathway.

Author information

1
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA. Electronic address: max.burroughs@nih.gov.
2
Division of Cancer Stem Cell, National Cancer Center Research Institute, Tokyo, Japan.

Abstract

Over the past decade, modifications to microRNAs (miRNAs) via 3' end nucleotide addition have gone from a deep-sequencing curiosity to experimentally confirmed drivers of a range of regulatory activities. Here we overview the methods that have been deployed by researchers seeking to untangle these diverse functional roles and include characterizing not only the nucleotidyl transferases catalyzing the additions but also the nucleotides being added, and the timing of their addition during the miRNA pathway. These methods and their further development are key to clarifying the diverse and sometimes contradictory functional findings presently attributed to these nucleotide additions.

PMID:
30138674
PMCID:
PMC6311138
[Available on 2020-01-01]
DOI:
10.1016/j.ymeth.2018.08.006
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center