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Assessment of the quality of DNA from various formalin-fixed paraffin-embedded (FFPE) tissues and the use of this DNA for next-generation sequencing (NGS) with no artifactual mutation.

Einaga N, Yoshida A, Noda H, Suemitsu M, Nakayama Y, Sakurada A, Kawaji Y, Yamaguchi H, Sasaki Y, Tokino T, Esumi M.

PLoS One. 2017 May 12;12(5):e0176280. doi: 10.1371/journal.pone.0176280. eCollection 2017.


Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome.

Shen Y, Wang Y, Chen T, Gao F, Gong J, Abramczyk D, Walker R, Zhao H, Chen S, Liu W, Luo Y, Müller CA, Paul-Dubois-Taine A, Alver B, Stracquadanio G, Mitchell LA, Luo Z, Fan Y, Zhou B, Wen B, Tan F, Wang Y, Zi J, Xie Z, Li B, Yang K, Richardson SM, Jiang H, French CE, Nieduszynski CA, Koszul R, Marston AL, Yuan Y, Wang J, Bader JS, Dai J, Boeke JD, Xu X, Cai Y, Yang H.

Science. 2017 Mar 10;355(6329). pii: eaaf4791. doi: 10.1126/science.aaf4791.


Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts.

Ding J, Lin ZQ, Jiang JM, Seidman CE, Seidman JG, Pu WT, Wang DZ.

J Vis Exp. 2016 Dec 17;(118). doi: 10.3791/54787.


TrmBL2 from Pyrococcus furiosus Interacts Both with Double-Stranded and Single-Stranded DNA.

Wierer S, Daldrop P, Ud Din Ahmad M, Boos W, Drescher M, Welte W, Seidel R.

PLoS One. 2016 May 23;11(5):e0156098. doi: 10.1371/journal.pone.0156098. eCollection 2016.


Detection of HER2 Gene Polymorphism in Breast Cancer: PCR Optimization Study.

Budiarto BR, Desriani.

Sci Pharm. 2016 Feb 14;84(1):103-11. doi: 10.3797/scipharm.ISP.2015.03. Print 2016 Jan-Mar. Erratum in: Sci Pharm. 2016 Dec 02;84(4):753.


454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature.

Gonzalez-Martinez A, Rodriguez-Sanchez A, Rodelas B, Abbas BA, Martinez-Toledo MV, van Loosdrecht MC, Osorio F, Gonzalez-Lopez J.

Biomed Res Int. 2015;2015:892013. doi: 10.1155/2015/892013. Epub 2015 Sep 3.


Optimizing a PCR protocol for cpn60-based microbiome profiling of samples variously contaminated with host genomic DNA.

Johnson LA, Chaban B, Harding JC, Hill JE.

BMC Res Notes. 2015 Jun 20;8:253. doi: 10.1186/s13104-015-1170-4.


PrimerView: high-throughput primer design and visualization.

O'Halloran DM.

Source Code Biol Med. 2015 Jun 4;10:8. doi: 10.1186/s13029-015-0038-2. eCollection 2015.


Potential role for the Metnase transposase fusion gene in colon cancer through the regulation of key genes.

Apostolou P, Toloudi M, Kourtidou E, Mimikakou G, Vlachou I, Chatziioannou M, Kipourou V, Papasotiriou I.

PLoS One. 2014 Oct 15;9(10):e109741. doi: 10.1371/journal.pone.0109741. eCollection 2014.


Sequence-dependent biophysical modeling of DNA amplification.

Marimuthu K, Jing C, Chakrabarti R.

Biophys J. 2014 Oct 7;107(7):1731-43. doi: 10.1016/j.bpj.2014.08.019.


Reagentless, ratiometric electrochemical DNA sensors with improved robustness and reproducibility.

Du Y, Lim BJ, Li B, Jiang YS, Sessler JL, Ellington AD.

Anal Chem. 2014 Aug 5;86(15):8010-6. doi: 10.1021/ac5025254. Epub 2014 Jul 18.


Evaluation of the mtDNA-COII Region Based Species Specific Assay for Identifying Members of the Anopheles culicifacies Species Complex.

Manonmani AM, Mathivanan AK, Sadanandane C, Jambulingam P.

J Arthropod Borne Dis. 2013 Aug 31;7(2):154-63. eCollection 2013.


Development of a highly sensitive immuno-PCR assay for the measurement of α-galactosidase A protein levels in serum and plasma.

Nakano S, Morizane Y, Makisaka N, Suzuki T, Togawa T, Tsukimura T, Kawashima I, Sakuraba H, Shibasaki F.

PLoS One. 2013 Nov 13;8(11):e78588. doi: 10.1371/journal.pone.0078588. eCollection 2013.


High-throughput system for the presentation of secreted and surface-exposed proteins from Gram-positive bacteria in functional metagenomics studies.

Dobrijevic D, Di Liberto G, Tanaka K, de Wouters T, Dervyn R, Boudebbouze S, Binesse J, Blottière HM, Jamet A, Maguin E, van de Guchte M.

PLoS One. 2013 Jun 14;8(6):e65956. doi: 10.1371/journal.pone.0065956. Print 2013.


Direct assessment of viral diversity in soils by random PCR amplification of polymorphic DNA.

Srinivasiah S, Lovett J, Polson S, Bhavsar J, Ghosh D, Roy K, Fuhrmann JJ, Radosevich M, Wommack KE.

Appl Environ Microbiol. 2013 Sep;79(18):5450-7. doi: 10.1128/AEM.00268-13. Epub 2013 Jun 21.


PCR on yeast colonies: an improved method for glyco-engineered Saccharomyces cerevisiae.

Bonnet C, Rigaud C, Chanteclaire E, Blandais C, Tassy-Freches E, Arico C, Javaud C.

BMC Res Notes. 2013 May 20;6:201. doi: 10.1186/1756-0500-6-201.


Whole-genome thermodynamic analysis reduces siRNA off-target effects.

Chen X, Liu P, Chou HH.

PLoS One. 2013;8(3):e58326. doi: 10.1371/journal.pone.0058326. Epub 2013 Mar 6.


Novel multiregion hybridization assay for the identification of the most prevalent genetic forms of the human immunodeficiency virus type 1 circulating in Portugal.

Freitas FB, Esteves A, Piedade J, Parreira R.

AIDS Res Hum Retroviruses. 2013 Feb;29(2):318-28. doi: 10.1089/AID.2012.0025. Epub 2012 Sep 25.


Rapid hybridization of nucleic acids using isotachophoresis.

Bercovici M, Han CM, Liao JC, Santiago JG.

Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11127-32. doi: 10.1073/pnas.1205004109. Epub 2012 Jun 25.


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