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Items: 1 to 20 of 31


Full and Partial Agonism of a Designed Enzyme Switch.

Budiardjo SJ, Licknack TJ, Cory MB, Kapros D, Roy A, Lovell S, Douglas J, Karanicolas J.

ACS Synth Biol. 2016 Dec 16;5(12):1475-1484. Epub 2016 Jul 22.


An Unprecedented Combination of Serine and Cysteine Nucleophiles in a Split Intein with an Atypical Split Site.

Bachmann AL, Mootz HD.

J Biol Chem. 2015 Nov 27;290(48):28792-804. doi: 10.1074/jbc.M115.677237. Epub 2015 Oct 9.


De novo assembly and next-generation sequencing to analyse full-length gene variants from codon-barcoded libraries.

Cho N, Hwang B, Yoon JK, Park S, Lee J, Seo HN, Lee J, Huh S, Chung J, Bang D.

Nat Commun. 2015 Sep 21;6:8351. doi: 10.1038/ncomms9351.


Mammalian synthetic biology: emerging medical applications.

Kis Z, Pereira HS, Homma T, Pedrigi RM, Krams R.

J R Soc Interface. 2015 May 6;12(106). pii: 20141000. doi: 10.1098/rsif.2014.1000. Review.


A split intein T7 RNA polymerase for transcriptional AND-logic.

Schaerli Y, Gili M, Isalan M.

Nucleic Acids Res. 2014 Oct 29;42(19):12322-8. doi: 10.1093/nar/gku884. Epub 2014 Sep 27.


Ion channel engineering: perspectives and strategies.

Subramanyam P, Colecraft HM.

J Mol Biol. 2015 Jan 16;427(1):190-204. doi: 10.1016/j.jmb.2014.09.001. Epub 2014 Sep 7. Review.


Nature's recipe for splitting inteins.

Aranko AS, Wlodawer A, Iwaï H.

Protein Eng Des Sel. 2014 Aug;27(8):263-71. doi: 10.1093/protein/gzu028. Review.


Structure of the branched intermediate in protein splicing.

Liu Z, Frutos S, Bick MJ, Vila-Perelló M, Debelouchina GT, Darst SA, Muir TW.

Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8422-7. doi: 10.1073/pnas.1402942111. Epub 2014 Apr 28.


Structural and dynamical features of inteins and implications on protein splicing.

Eryilmaz E, Shah NH, Muir TW, Cowburn D.

J Biol Chem. 2014 May 23;289(21):14506-11. doi: 10.1074/jbc.R113.540302. Epub 2014 Apr 2. Review.


Inteins: Nature's Gift to Protein Chemists.

Shah NH, Muir TW.

Chem Sci. 2014;5(1):446-461.


Synthesis of an intein-mediated artificial protein hydrogel.

Ramirez MA, Chen Z.

J Vis Exp. 2014 Jan 27;(83):e51202. doi: 10.3791/51202.


Steps towards the synthetic biology of polyketide biosynthesis.

Cummings M, Breitling R, Takano E.

FEMS Microbiol Lett. 2014 Feb;351(2):116-25. doi: 10.1111/1574-6968.12365. Epub 2014 Jan 7. Review.


SPLICEFINDER - a fast and easy screening method for active protein trans-splicing positions.

Zettler J, Eppmann S, Busche A, Dikovskaya D, Dötsch V, Mootz HD, Sonntag T.

PLoS One. 2013 Sep 2;8(9):e72925. doi: 10.1371/journal.pone.0072925. eCollection 2013.


Manipulating L-type calcium channels in cardiomyocytes using split-intein protein transsplicing.

Subramanyam P, Chang DD, Fang K, Xie W, Marks AR, Colecraft HM.

Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15461-6. doi: 10.1073/pnas.1308161110. Epub 2013 Sep 3.


Tuning the dials of Synthetic Biology.

Arpino JA, Hancock EJ, Anderson J, Barahona M, Stan GB, Papachristodoulou A, Polizzi K.

Microbiology. 2013 Jul;159(Pt 7):1236-53. doi: 10.1099/mic.0.067975-0. Epub 2013 May 23. Review.


Synthetic biology of antimicrobial discovery.

Zakeri B, Lu TK.

ACS Synth Biol. 2013 Jul 19;2(7):358-72. doi: 10.1021/sb300101g. Epub 2012 Dec 4. Review.


Split Inteins: Nature's Protein Ligases.

Shah NH, Muir TW.

Isr J Chem. 2011 Nov 1;51(8-9):854-861.


Extein residues play an intimate role in the rate-limiting step of protein trans-splicing.

Shah NH, Eryilmaz E, Cowburn D, Muir TW.

J Am Chem Soc. 2013 Apr 17;135(15):5839-47. doi: 10.1021/ja401015p. Epub 2013 Apr 2.


Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues.

Cheriyan M, Pedamallu CS, Tori K, Perler F.

J Biol Chem. 2013 Mar 1;288(9):6202-11. doi: 10.1074/jbc.M112.433094. Epub 2013 Jan 10.


Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources.

Carvajal-Vallejos P, Pallissé R, Mootz HD, Schmidt SR.

J Biol Chem. 2012 Aug 17;287(34):28686-96. doi: 10.1074/jbc.M112.372680. Epub 2012 Jun 28.

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