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Items: 1 to 20 of 136


An intragenic mutagenesis strategy in Physcomitrella patens to preserve intron splicing.

Ako AE, Perroud PF, Innocent J, Demko V, Olsen OA, Johansen W.

Sci Rep. 2017 Jul 11;7(1):5111. doi: 10.1038/s41598-017-05309-w.


Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals.

Yu T, Zhou YJ, Wenning L, Liu Q, Krivoruchko A, Siewers V, Nielsen J, David F.

Nat Commun. 2017 May 26;8:15587. doi: 10.1038/ncomms15587.


Automated multiplex genome-scale engineering in yeast.

Si T, Chao R, Min Y, Wu Y, Ren W, Zhao H.

Nat Commun. 2017 May 4;8:15187. doi: 10.1038/ncomms15187.


A plug-and-play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae.

Ren H, Hu P, Zhao H.

Biotechnol Bioeng. 2017 Aug;114(8):1847-1854. doi: 10.1002/bit.26309. Epub 2017 Jun 5.


Scarless assembly of unphosphorylated DNA fragments with a simplified DATEL method.

Ding W, Weng H, Jin P, Du G, Chen J, Kang Z.

Bioengineered. 2017 May 4;8(3):296-301. doi: 10.1080/21655979.2017.1308986.


Improving acetyl-CoA biosynthesis in Saccharomyces cerevisiae via the overexpression of pantothenate kinase and PDH bypass.

Liu W, Zhang B, Jiang R.

Biotechnol Biofuels. 2017 Feb 17;10:41. doi: 10.1186/s13068-017-0726-z. eCollection 2017.


Engineering dynamical control of cell fate switching using synthetic phospho-regulons.

Gordley RM, Williams RE, Bashor CJ, Toettcher JE, Yan S, Lim WA.

Proc Natl Acad Sci U S A. 2016 Nov 22;113(47):13528-13533. Epub 2016 Nov 7.


Metabolic engineering of Saccharomyces cerevisiae for de novo production of dihydrochalcones with known antioxidant, antidiabetic, and sweet tasting properties.

Eichenberger M, Lehka BJ, Folly C, Fischer D, Martens S, Simón E, Naesby M.

Metab Eng. 2017 Jan;39:80-89. doi: 10.1016/j.ymben.2016.10.019. Epub 2016 Oct 31.


A robust gene-stacking method utilizing yeast assembly for plant synthetic biology.

Shih PM, Vuu K, Mansoori N, Ayad L, Louie KB, Bowen BP, Northen TR, Loqué D.

Nat Commun. 2016 Oct 26;7:13215. doi: 10.1038/ncomms13215.


The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production.

Alcaíno J, Bravo N, Córdova P, Marcoleta AE, Contreras G, Barahona S, Sepúlveda D, Fernández-Lobato M, Baeza M, Cifuentes V.

PLoS One. 2016 Sep 13;11(9):e0162838. doi: 10.1371/journal.pone.0162838. eCollection 2016.


Discovery and Reconstitution of the Cycloclavine Biosynthetic Pathway-Enzymatic Formation of a Cyclopropyl Group.

Jakubczyk D, Caputi L, Hatsch A, Nielsen CA, Diefenbacher M, Klein J, Molt A, Schröder H, Cheng JZ, Naesby M, O'Connor SE.

Angew Chem Weinheim Bergstr Ger. 2015 Apr 20;127(17):5206-5210. Epub 2015 Feb 25.


Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines.

McKeague M, Wang YH, Cravens A, Win MN, Smolke CD.

Metab Eng. 2016 Nov;38:191-203. doi: 10.1016/j.ymben.2016.08.003. Epub 2016 Aug 9.


High-throughput evaluation of synthetic metabolic pathways.

Klesmith JR, Whitehead TA.

Technology (Singap World Sci). 2016 Mar;4(1):9-14. Epub 2015 Dec 16.


Engineering biosynthesis of the anticancer alkaloid noscapine in yeast.

Li Y, Smolke CD.

Nat Commun. 2016 Jul 5;7:12137. doi: 10.1038/ncomms12137.


Engineering of synthetic, stress-responsive yeast promoters.

Rajkumar AS, Liu G, Bergenholm D, Arsovska D, Kristensen M, Nielsen J, Jensen MK, Keasling JD.

Nucleic Acids Res. 2016 Sep 30;44(17):e136. doi: 10.1093/nar/gkw553. Epub 2016 Jun 20.


CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA.

Zhou J, Wu R, Xue X, Qin Z.

Nucleic Acids Res. 2016 Aug 19;44(14):e124. doi: 10.1093/nar/gkw475. Epub 2016 May 24.

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