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Items: 1 to 20 of 43


Interrogation of γ-tubulin alleles using high-resolution fitness measurements reveals a distinct cytoplasmic function in spindle alignment.

Shulist K, Yen E, Kaitna S, Leary A, Decterov A, Gupta D, Vogel J.

Sci Rep. 2017 Sep 12;7(1):11398. doi: 10.1038/s41598-017-11789-7.


Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method.

Liang J, Liu Z, Low XZ, Ang EL, Zhao H.

Nucleic Acids Res. 2017 Jun 20;45(11):e94. doi: 10.1093/nar/gkx132.


Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome.

Shen Y, Wang Y, Chen T, Gao F, Gong J, Abramczyk D, Walker R, Zhao H, Chen S, Liu W, Luo Y, Müller CA, Paul-Dubois-Taine A, Alver B, Stracquadanio G, Mitchell LA, Luo Z, Fan Y, Zhou B, Wen B, Tan F, Wang Y, Zi J, Xie Z, Li B, Yang K, Richardson SM, Jiang H, French CE, Nieduszynski CA, Koszul R, Marston AL, Yuan Y, Wang J, Bader JS, Dai J, Boeke JD, Xu X, Cai Y, Yang H.

Science. 2017 Mar 10;355(6329). pii: eaaf4791. doi: 10.1126/science.aaf4791.


Optimization of Engineered Production of the Glucoraphanin Precursor Dihomomethionine in Nicotiana benthamiana.

Crocoll C, Mirza N, Reichelt M, Gershenzon J, Halkier BA.

Front Bioeng Biotechnol. 2016 Feb 16;4:14. doi: 10.3389/fbioe.2016.00014. eCollection 2016.


AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach.

Beyer HM, Gonschorek P, Samodelov SL, Meier M, Weber W, Zurbriggen MD.

PLoS One. 2015 Sep 11;10(9):e0137652. doi: 10.1371/journal.pone.0137652. eCollection 2015.


New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.

Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, Wyrick JJ.

Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21.


Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells.

Brandt B, Munemasa S, Wang C, Nguyen D, Yong T, Yang PG, Poretsky E, Belknap TF, Waadt R, Alemán F, Schroeder JI.

Elife. 2015 Jul 20;4. doi: 10.7554/eLife.03599. Erratum in: Elife. 2015;4:e10328.


PaperClip: rapid multi-part DNA assembly from existing libraries.

Trubitsyna M, Michlewski G, Cai Y, Elfick A, French CE.

Nucleic Acids Res. 2014 Nov 10;42(20):e154. doi: 10.1093/nar/gku829. Epub 2014 Sep 8.


A versatile system for USER cloning-based assembly of expression vectors for mammalian cell engineering.

Lund AM, Kildegaard HF, Petersen MB, Rank J, Hansen BG, Andersen MR, Mortensen UH.

PLoS One. 2014 May 30;9(5):e96693. doi: 10.1371/journal.pone.0096693. eCollection 2014.


A novel approach to the generation of seamless constructs for plant transformation.

Kronbak R, Ingvardsen CR, Madsen CK, Gregersen PL.

Plant Methods. 2014 May 10;10:10. doi: 10.1186/1746-4811-10-10. eCollection 2014.


Microtubules accelerate the kinase activity of Aurora-B by a reduction in dimensionality.

Noujaim M, Bechstedt S, Wieczorek M, Brouhard GJ.

PLoS One. 2014 Feb 3;9(2):e86786. doi: 10.1371/journal.pone.0086786. eCollection 2014.


DNA assembly techniques for next-generation combinatorial biosynthesis of natural products.

Cobb RE, Ning JC, Zhao H.

J Ind Microbiol Biotechnol. 2014 Feb;41(2):469-77. doi: 10.1007/s10295-013-1358-3. Epub 2013 Oct 15. Review.


Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V.

Baumann T, Arndt KM, Müller KM.

BMC Biotechnol. 2013 Oct 4;13:81. doi: 10.1186/1472-6750-13-81.


Emerging tools for synthetic genome design.

Lee BR, Cho S, Song Y, Kim SC, Cho BK.

Mol Cells. 2013 May;35(5):359-70. doi: 10.1007/s10059-013-0127-5. Epub 2013 May 2. Review.


Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex.

He J, Chao WC, Zhang Z, Yang J, Cronin N, Barford D.

Mol Cell. 2013 Jun 6;50(5):649-60. doi: 10.1016/j.molcel.2013.04.024. Epub 2013 May 23.


A survey of enabling technologies in synthetic biology.

Kahl LJ, Endy D.

J Biol Eng. 2013 May 10;7(1):13. doi: 10.1186/1754-1611-7-13.


DNA fragments assembly based on nicking enzyme system.

Wang RY, Shi ZY, Guo YY, Chen JC, Chen GQ.

PLoS One. 2013;8(3):e57943. doi: 10.1371/journal.pone.0057943. Epub 2013 Mar 6.


Parallel in vivo DNA assembly by recombination: experimental demonstration and theoretical approaches.

Shi Z, Wedd AG, Gras SL.

PLoS One. 2013;8(2):e56854. doi: 10.1371/journal.pone.0056854. Epub 2013 Feb 28.


PGASO: A synthetic biology tool for engineering a cellulolytic yeast.

Chang JJ, Ho CY, Ho FJ, Tsai TY, Ke HM, Wang CH, Chen HL, Shih MC, Huang CC, Li WH.

Biotechnol Biofuels. 2012 Jul 27;5(1):53. doi: 10.1186/1754-6834-5-53.


Doublecortin recognizes the 13-protofilament microtubule cooperatively and tracks microtubule ends.

Bechstedt S, Brouhard GJ.

Dev Cell. 2012 Jul 17;23(1):181-92. doi: 10.1016/j.devcel.2012.05.006. Epub 2012 Jun 21.

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