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Items: 7

1.

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy.

Görlitz F, Kelly DJ, Warren SC, Alibhai D, West L, Kumar S, Alexandrov Y, Munro I, Garcia E, McGinty J, Talbot C, Serwa RA, Thinon E, da Paola V, Murray EJ, Stuhmeier F, Neil MA, Tate EW, Dunsby C, French PM.

J Vis Exp. 2017 Jan 18;(119). doi: 10.3791/55119.

2.

Measurement of inositol 1,4,5-trisphosphate in living cells using an improved set of resonance energy transfer-based biosensors.

Gulyás G, Tóth JT, Tóth DJ, Kurucz I, Hunyady L, Balla T, Várnai P.

PLoS One. 2015 May 1;10(5):e0125601. doi: 10.1371/journal.pone.0125601. eCollection 2015.

3.

Quantitative tomographic imaging of intermolecular FRET in small animals.

Venugopal V, Chen J, Barroso M, Intes X.

Biomed Opt Express. 2012 Dec 1;3(12):3161-75. doi: 10.1364/BOE.3.003161. Epub 2012 Nov 8.

4.

Automated fluorescence lifetime imaging plate reader and its application to Förster resonant energy transfer readout of Gag protein aggregation.

Alibhai D, Kelly DJ, Warren S, Kumar S, Margineau A, Serwa RA, Thinon E, Alexandrov Y, Murray EJ, Stuhmeier F, Tate EW, Neil MA, Dunsby C, French PM.

J Biophotonics. 2013 May;6(5):398-408. doi: 10.1002/jbio.201200185. Epub 2012 Nov 26.

5.

In vivo fluorescence lifetime optical projection tomography.

McGinty J, Taylor HB, Chen L, Bugeon L, Lamb JR, Dallman MJ, French PM.

Biomed Opt Express. 2011 Apr 26;2(5):1340-50. doi: 10.1364/BOE.2.001340.

6.

FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ.

Kumar S, Alibhai D, Margineanu A, Laine R, Kennedy G, McGinty J, Warren S, Kelly D, Alexandrov Y, Munro I, Talbot C, Stuckey DW, Kimberly C, Viellerobe B, Lacombe F, Lam EW, Taylor H, Dallman MJ, Stamp G, Murray EJ, Stuhmeier F, Sardini A, Katan M, Elson DS, Neil MA, Dunsby C, French PM.

Chemphyschem. 2011 Feb 25;12(3):609-26. doi: 10.1002/cphc.201000874. Epub 2011 Feb 17.

7.

Use of Fluorescence Resonance Energy Transfer-based Biosensors for the Quantitative Analysis of Inositol 1,4,5-Trisphosphate Dynamics in Calcium Oscillations.

Tanimura A, Morita T, Nezu A, Shitara A, Hashimoto N, Tojyo Y.

J Biol Chem. 2009 Mar 27;284(13):8910-7. doi: 10.1074/jbc.M805865200. Epub 2009 Jan 21.

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