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Items: 1 to 20 of 32


Coordinated protein co-expression in plants by harnessing the synergy between an intein and a viral 2A peptide.

Zhang B, Rapolu M, Kumar S, Gupta M, Liang Z, Han Z, Williams P, Su WW.

Plant Biotechnol J. 2017 Jun;15(6):718-728. doi: 10.1111/pbi.12670. Epub 2017 Mar 30.


Sequential formation of two branched intermediates during protein splicing of class three inteins.

Tori K, Perler F.

Extremophiles. 2017 Jan;21(1):41-49. doi: 10.1007/s00792-016-0876-0. Epub 2016 Oct 4.


Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes.

Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G.

Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.


Nature's recipe for splitting inteins.

Aranko AS, Wlodawer A, Iwaï H.

Protein Eng Des Sel. 2014 Aug;27(8):263-71. doi: 10.1093/protein/gzu028. Review.


Intein applications: from protein purification and labeling to metabolic control methods.

Wood DW, Camarero JA.

J Biol Chem. 2014 May 23;289(21):14512-9. doi: 10.1074/jbc.R114.552653. Epub 2014 Apr 2. Review.


Protein splicing: how inteins escape from precursor proteins.

Mills KV, Johnson MA, Perler FB.

J Biol Chem. 2014 May 23;289(21):14498-505. doi: 10.1074/jbc.R113.540310. Epub 2014 Apr 2. Review.


Oriented covalent immobilization of antibodies for measurement of intermolecular binding forces between zipper-like contact surfaces of split inteins.

Sorci M, Dassa B, Liu H, Anand G, Dutta AK, Pietrokovski S, Belfort M, Belfort G.

Anal Chem. 2013 Jun 18;85(12):6080-8. doi: 10.1021/ac400949t. Epub 2013 May 31.


Protein trans-splicing of multiple atypical split inteins engineered from natural inteins.

Lin Y, Li M, Song H, Xu L, Meng Q, Liu XQ.

PLoS One. 2013 Apr 8;8(4):e59516. doi: 10.1371/journal.pone.0059516. Print 2013.


Extein residues play an intimate role in the rate-limiting step of protein trans-splicing.

Shah NH, Eryilmaz E, Cowburn D, Muir TW.

J Am Chem Soc. 2013 Apr 17;135(15):5839-47. doi: 10.1021/ja401015p. Epub 2013 Apr 2.


Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues.

Cheriyan M, Pedamallu CS, Tori K, Perler F.

J Biol Chem. 2013 Mar 1;288(9):6202-11. doi: 10.1074/jbc.M112.433094. Epub 2013 Jan 10.


Circular permutation prediction reveals a viable backbone disconnection for split proteins: an approach in identifying a new functional split intein.

Lee YT, Su TH, Lo WC, Lyu PC, Sue SC.

PLoS One. 2012;7(8):e43820. doi: 10.1371/journal.pone.0043820. Epub 2012 Aug 24.


Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources.

Carvajal-Vallejos P, Pallissé R, Mootz HD, Schmidt SR.

J Biol Chem. 2012 Aug 17;287(34):28686-96. doi: 10.1074/jbc.M112.372680. Epub 2012 Jun 28.


Ultrafast protein splicing is common among cyanobacterial split inteins: implications for protein engineering.

Shah NH, Dann GP, Vila-Perelló M, Liu Z, Muir TW.

J Am Chem Soc. 2012 Jul 18;134(28):11338-41. doi: 10.1021/ja303226x. Epub 2012 Jul 2.


Mutual synergistic protein folding in split intein.

Zheng Y, Wu Q, Wang C, Xu MQ, Liu Y.

Biosci Rep. 2012 Oct;32(5):433-42. doi: 10.1042/BSR20120049.


In-cell fluorescence activation and labeling of proteins mediated by FRET-quenched split inteins.

Borra R, Dong D, Elnagar AY, Woldemariam GA, Camarero JA.

J Am Chem Soc. 2012 Apr 11;134(14):6344-53. doi: 10.1021/ja300209u. Epub 2012 Mar 28.


Branched intermediate formation is the slowest step in the protein splicing reaction of the Ala1 KlbA intein from Methanococcus jannaschii.

Saleh L, Southworth MW, Considine N, O'Neill C, Benner J, Bollinger JM Jr, Perler FB.

Biochemistry. 2011 Dec 13;50(49):10576-89. doi: 10.1021/bi200810j. Epub 2011 Nov 16.


Use of protein trans-splicing to produce active and segmentally (2)H, (15)N labeled mannuronan C5-epimerase AlgE4.

Buchinger E, Aachmann FL, Aranko AS, Valla S, Skjåk-Braek G, Iwaï H, Wimmer R.

Protein Sci. 2010 Aug;19(8):1534-43. doi: 10.1002/pro.432.


Photomodulation of protein trans-splicing through backbone photocaging of the DnaE split intein.

Berrade L, Kwon Y, Camarero JA.

Chembiochem. 2010 Jul 5;11(10):1368-72. doi: 10.1002/cbic.201000157. No abstract available.


Branched intermediate formation stimulates peptide bond cleavage in protein splicing.

Frutos S, Goger M, Giovani B, Cowburn D, Muir TW.

Nat Chem Biol. 2010 Jul;6(7):527-33. doi: 10.1038/nchembio.371. Epub 2010 May 23.


Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein.

Volkmann G, Liu XQ.

PLoS One. 2009 Dec 21;4(12):e8381. doi: 10.1371/journal.pone.0008381.

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