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Items: 1 to 20 of 308

1.

Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step.

Chong S, Montello GE, Zhang A, Cantor EJ, Liao W, Xu MQ, Benner J.

Nucleic Acids Res. 1998 Nov 15;26(22):5109-15.

2.

BPTI and N-terminal extended analogues generated by factor Xa cleavage and cathepsin C trimming of a fusion protein expressed in Escherichia coli.

Lauritzen C, Tüchsen E, Hansen PE, Skovgaard O.

Protein Expr Purif. 1991 Oct-Dec;2(5-6):372-8.

PMID:
1821811
3.
4.

Human glutaredoxin 2 affinity tag for recombinant peptide and protein purification.

Lundberg M, Holmgren A, Johansson M.

Protein Expr Purif. 2006 Jan;45(1):37-42. Epub 2005 Jul 27.

PMID:
16112873
5.

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element.

Chong S, Mersha FB, Comb DG, Scott ME, Landry D, Vence LM, Perler FB, Benner J, Kucera RB, Hirvonen CA, Pelletier JJ, Paulus H, Xu MQ.

Gene. 1997 Jun 19;192(2):271-81.

PMID:
9224900
6.

Construction of a high sensitive Escherichia coli alkaline phosphatase reporter system for screening affinity peptides.

Huang X, Zhang XE, Zhou YF, Zhang ZP, Cass AE.

J Biochem Biophys Methods. 2007 Apr 10;70(3):435-9. Epub 2006 Oct 21.

PMID:
17156847
7.

Esterase 2 from Alicyclobacillus acidocaldarius as a reporter and affinity tag for expression and single step purification of polypeptides.

Huang Y, Humenik M, Sprinzl M.

Protein Expr Purif. 2007 Jul;54(1):94-100. Epub 2007 Feb 17.

PMID:
17399994
8.

Construction of a dual-tag system for gene expression, protein affinity purification and fusion protein processing.

Motejadded H, Altenbuchner J.

Biotechnol Lett. 2009 Apr;31(4):543-9. doi: 10.1007/s10529-008-9909-9. Epub 2009 Jan 7.

PMID:
19127343
9.

Design of affinity tags for one-step protein purification from immobilized zinc columns.

Pasquinelli RS, Shepherd RE, Koepsel RR, Zhao A, Ataai MM.

Biotechnol Prog. 2000 Jan-Feb;16(1):86-91.

PMID:
10662495
10.

PHB-intein-mediated protein purification strategy.

Gillies AR, Mahmoud RB, Wood DW.

Methods Mol Biol. 2009;498:173-83. doi: 10.1007/978-1-59745-196-3_12.

PMID:
18988026
11.
12.

Comparison of classical and affinity purification techniques of Mason-Pfizer monkey virus capsid protein: the alteration of the product by an affinity tag.

Rumlová M, Benedíková J, Cubínková R, Pichová I, Ruml T.

Protein Expr Purif. 2001 Oct;23(1):75-83.

PMID:
11570848
13.

Self-cleavable stimulus responsive tags for protein purification without chromatography.

Ge X, Yang DS, Trabbic-Carlson K, Kim B, Chilkoti A, Filipe CD.

J Am Chem Soc. 2005 Aug 17;127(32):11228-9.

PMID:
16089436
14.

A micro-scale process for high-throughput expression of cDNAs in the yeast Saccharomyces cerevisiae.

Holz C, Hesse O, Bolotina N, Stahl U, Lang C.

Protein Expr Purif. 2002 Aug;25(3):372-8.

PMID:
12182816
15.

A modular set of prokaryotic and eukaryotic expression vectors.

Melcher K.

Anal Biochem. 2000 Jan 1;277(1):109-20. Erratum in: Anal Biochem 2000 Jul 1;282(2):266.

PMID:
10610695
16.

Use of Ssp dnaB derived mini-intein as a fusion partner for production of recombinant human brain natriuretic peptide in Escherichia coli.

Sun Z, Chen J, Yao H, Liu L, Wang J, Zhang J, Liu JN.

Protein Expr Purif. 2005 Sep;43(1):26-32.

PMID:
15979896
17.

An inducible expression system of histidine-tagged proteins in Streptomyces lividans for one-step purification by Ni2+ affinity chromatography.

Enguita FJ, de la Fuente JL, Martín JF, Liras P.

FEMS Microbiol Lett. 1996 Apr 1;137(2-3):135-40.

PMID:
8998975
18.

Fusion protein system designed to provide color to aid in the expression and purification of proteins in Escherichia coli.

Park KW, Webster DA, Stark BC, Howard AJ, Kim KJ.

Plasmid. 2003 Nov;50(3):169-75.

PMID:
14597006
19.

Highly efficient protein expression and purification using bacterial hemoglobin fusion vector.

Kwon SY, Choi YJ, Kang TH, Lee KH, Cha SS, Kim GH, Lee HS, Kim KT, Kim KJ.

Plasmid. 2005 May;53(3):274-82. Epub 2005 Jan 20.

PMID:
15848232
20.

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