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Items: 1 to 20 of 73

1.

PCR amplification of antibody variable regions using primers that anneal to constant regions.

Sassano M, Repetto M, Cassani G, Corti A.

Nucleic Acids Res. 1994 May 11;22(9):1768-9. No abstract available.

2.

Inverse polymerase chain reaction for cloning complete human immunoglobulin variable regions and leaders conserving the original sequence.

Perfetti V, Sassano M, Ubbiali P, Vignarelli MC, Arbustini E, Corti A, Merlini G.

Anal Biochem. 1996 Jul 15;239(1):107-9. No abstract available.

PMID:
8660632
3.

Rapid PCR construction of a gene containing Lym-1 antibody variable regions.

Shi XB, Gumerlock PH, Wellman AA, Meares CF, DeNardo GL, Meyers FJ, DeNardo SJ.

PCR Methods Appl. 1993 Aug;3(1):46-53. No abstract available.

4.
5.

Phage-display libraries of murine and human antibody Fab fragments.

Engberg J, Andersen PS, Nielsen LK, Dziegiel M, Johansen LK, Albrechtsen B.

Methods Mol Biol. 1995;51:355-76. No abstract available.

PMID:
7581709
6.

Cloning, expression, and modification of antibody V regions.

Morrison SL.

Curr Protoc Immunol. 2002 May;Chapter 2:Unit 2.12. doi: 10.1002/0471142735.im0212s47.

PMID:
18432877
7.

Engineering of antibodies with a known three-dimensional structure.

Plückthun A, Glockshuber R, Pfitzinger I, Skerra A, Stadlmüller J.

Cold Spring Harb Symp Quant Biol. 1987;52:105-12. No abstract available.

PMID:
3454257
8.

Rapid PCR-cloning of full-length mouse immunoglobulin variable regions.

Jones ST, Bendig MM.

Biotechnology (N Y). 1991 Jan;9(1):88-9. No abstract available. Erratum in: Biotechnology (N Y). 1991 Jun;9(6):579.

PMID:
1367218
9.

Optimization of primers for cloning libraries of mouse immunoglobulin genes using the polymerase chain reaction.

Kettleborough CA, Saldanha J, Ansell KH, Bendig MM.

Eur J Immunol. 1993 Jan;23(1):206-11.

PMID:
8419173
10.

Mouse/human chimeric Me1-14 antibody: genomic cloning of the variable region genes, linkage to human constant region genes, expression, and characterization.

Batra SK, Niswonger ML, Wikstrand CJ, Pegram CN, Zalutsky MR, Morrison SL, Bigner DD.

Hybridoma. 1994 Apr;13(2):87-97.

PMID:
8050781
11.
12.

Molecular genetics of human anti-Rh antibodies.

Chouchane L, Breyer J, Van Spronsen A, Guillaume JL, Goossens D, Rouger P, Strosberg AD.

Dev Biol Stand. 1990;71:9-14. No abstract available.

PMID:
2119320
13.

Isolation of IgG antibody Fv-DNA from various mouse and rat hybridoma cell lines using the polymerase chain reaction with a simple set of primers.

Dübel S, Breitling F, Fuchs P, Zewe M, Gotter S, Welschof M, Moldenhauer G, Little M.

J Immunol Methods. 1994 Sep 30;175(1):89-95.

PMID:
7930642
14.

Primer design for the cloning of immunoglobulin heavy-chain leader-variable regions from mouse hybridoma cells using the PCR.

Coloma MJ, Larrick JW, Ayala M, Gavilondo-Cowley JV.

Biotechniques. 1991 Aug;11(2):152-4, 156.

PMID:
1931008
15.

Chimeric antibody with human constant regions and mouse variable regions directed against carcinoma-associated antigen 17-1A.

Sun LK, Curtis P, Rakowicz-Szulczynska E, Ghrayeb J, Chang N, Morrison SL, Koprowski H.

Proc Natl Acad Sci U S A. 1987 Jan;84(1):214-8.

16.

Design and analysis of PCR primers for the amplification and cloning of human immunoglobulin Fab fragments.

de Boer M, Chang SY, Eichinger G, Wong HC.

Hum Antibodies Hybridomas. 1994;5(1-2):57-64.

PMID:
7858184
17.

The use of the RACE method to clone hybridoma cDNA when V region primers fail.

Ruberti F, Cattaneo A, Bradbury A.

J Immunol Methods. 1994 Jul 12;173(1):33-9.

PMID:
8034983
18.
19.

PCR amplification of the functional immunoglobulin heavy chain variable gene from a hybridoma in the presence of two aberrant transcripts.

Irani Y, Tea M, Tilton RG, Coster DJ, Williams KA, Brereton HM.

J Immunol Methods. 2008 Jul 31;336(2):246-50. doi: 10.1016/j.jim.2008.04.014. Epub 2008 May 16.

PMID:
18538340
20.

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