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Items: 1 to 20 of 104

1.

Mind the gap: Micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae.

Wolff G, Limpens RWAL, Zheng S, Snijder EJ, Agard DA, Koster AJ, Bárcena M.

J Struct Biol. 2019 Dec 1;208(3). pii: S1047-8477(19)30200-X. doi: 10.1016/j.jsb.2019.09.006. Epub 2019 Sep 16.

2.

Robust workflow and instrumentation for cryo-focused ion beam milling of samples for electron cryotomography.

Medeiros JM, Böck D, Weiss GL, Kooger R, Wepf RA, Pilhofer M.

Ultramicroscopy. 2018 Jul;190:1-11. doi: 10.1016/j.ultramic.2018.04.002. Epub 2018 Apr 6.

PMID:
29655973
3.

PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy.

Gorelick S, Buckley G, Gervinskas G, Johnson TK, Handley A, Caggiano MP, Whisstock JC, Pocock R, de Marco A.

Elife. 2019 Jul 1;8. pii: e45919. doi: 10.7554/eLife.45919.

4.

Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM.

He J, Hsieh C, Wu Y, Schmelzer T, Wang P, Lin Y, Marko M, Sui H.

J Struct Biol. 2017 Aug;199(2):114-119. doi: 10.1016/j.jsb.2017.05.011. Epub 2017 May 27.

5.

An improved cryo-FIB method for fabrication of frozen hydrated lamella.

Zhang J, Ji G, Huang X, Xu W, Sun F.

J Struct Biol. 2016 May;194(2):218-23. doi: 10.1016/j.jsb.2016.02.013. Epub 2016 Feb 12.

6.

Cryo-focused-ion-beam applications in structural biology.

Rigort A, Plitzko JM.

Arch Biochem Biophys. 2015 Sep 1;581:122-30. doi: 10.1016/j.abb.2015.02.009. Epub 2015 Feb 20. Review.

PMID:
25703192
7.

Optimized cryo-focused ion beam sample preparation aimed at in situ structural studies of membrane proteins.

Schaffer M, Mahamid J, Engel BD, Laugks T, Baumeister W, Plitzko JM.

J Struct Biol. 2017 Feb;197(2):73-82. doi: 10.1016/j.jsb.2016.07.010. Epub 2016 Jul 19.

PMID:
27444390
8.

Making the practically impossible "Merely difficult"--Cryogenic FIB lift-out for "Damage free" soft matter imaging.

Parmenter CD, Fay MW, Hartfield C, Eltaher HM.

Microsc Res Tech. 2016 Apr;79(4):298-303. doi: 10.1002/jemt.22630. Epub 2016 Feb 15.

PMID:
26875988
9.

In situ Microfluidic Cryofixation for Cryo Focused Ion Beam Milling and Cryo Electron Tomography.

Fuest M, Schaffer M, Nocera GM, Galilea-Kleinsteuber RI, Messling JE, Heymann M, Plitzko JM, Burg TP.

Sci Rep. 2019 Dec 13;9(1):19133. doi: 10.1038/s41598-019-55413-2.

10.

Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy.

Arnold J, Mahamid J, Lucic V, de Marco A, Fernandez JJ, Laugks T, Mayer T, Hyman AA, Baumeister W, Plitzko JM.

Biophys J. 2016 Feb 23;110(4):860-9. doi: 10.1016/j.bpj.2015.10.053. Epub 2016 Jan 5.

11.

Cryo-focused Ion Beam Sample Preparation for Imaging Vitreous Cells by Cryo-electron Tomography.

Schaffer M, Engel BD, Laugks T, Mahamid J, Plitzko JM, Baumeister W.

Bio Protoc. 2015 Sep 5;5(17). pii: e1575.

12.

Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography.

Hsieh C, Schmelzer T, Kishchenko G, Wagenknecht T, Marko M.

J Struct Biol. 2014 Jan;185(1):32-41. doi: 10.1016/j.jsb.2013.10.019. Epub 2013 Nov 6.

13.

In situ protein micro-crystal fabrication by cryo-FIB for electron diffraction.

Li X, Zhang S, Zhang J, Sun F.

Biophys Rep. 2018;4(6):339-347. doi: 10.1007/s41048-018-0075-x. Epub 2018 Nov 14.

15.
16.

3D structure determination of native mammalian cells using cryo-FIB and cryo-electron tomography.

Wang K, Strunk K, Zhao G, Gray JL, Zhang P.

J Struct Biol. 2012 Nov;180(2):318-26. doi: 10.1016/j.jsb.2012.07.003. Epub 2012 Jul 14.

17.

Cryo-electron microscopy specimen preparation by means of a focused ion beam.

Rubino S, Melin P, Spellward P, Leifer K.

J Vis Exp. 2014 Jul 26;(89):e51463. doi: 10.3791/51463.

18.

Cryo-electron tomography-the cell biology that came in from the cold.

Wagner J, Schaffer M, Fernández-Busnadiego R.

FEBS Lett. 2017 Sep;591(17):2520-2533. doi: 10.1002/1873-3468.12757. Epub 2017 Aug 2. Review.

19.

Thinning of large mammalian cells for cryo-TEM characterization by cryo-FIB milling.

Strunk KM, Wang K, Ke D, Gray JL, Zhang P.

J Microsc. 2012 Sep;247(3):220-7. doi: 10.1111/j.1365-2818.2012.03635.x.

20.

In-situ integrity control of frozen-hydrated, vitreous lamellas prepared by the cryo-focused ion beam-scanning electron microscope.

de Winter DA, Mesman RJ, Hayles MF, Schneijdenberg CT, Mathisen C, Post JA.

J Struct Biol. 2013 Jul;183(1):11-8. doi: 10.1016/j.jsb.2013.05.016. Epub 2013 Jun 4.

PMID:
23742839

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