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Cattle with a precise, zygote-mediated deletion safely eliminate the major milk allergen beta-lactoglobulin.

Wei J, Wagner S, Maclean P, Brophy B, Cole S, Smolenski G, Carlson DF, Fahrenkrug SC, Wells DN, Laible G.

Sci Rep. 2018 May 16;8(1):7661. doi: 10.1038/s41598-018-25654-8.


Production of hypoallergenic milk from DNA-free beta-lactoglobulin (BLG) gene knockout cow using zinc-finger nucleases mRNA.

Sun Z, Wang M, Han S, Ma S, Zou Z, Ding F, Li X, Li L, Tang B, Wang H, Li N, Che H, Dai Y.

Sci Rep. 2018 Oct 18;8(1):15430. doi: 10.1038/s41598-018-32024-x.


Influence of the route of exposure and the matrix on the sensitisation potency of a major cows' milk allergen.

Wavrin S, Bernard H, Wal JM, Adel-Patient K.

Clin Transl Allergy. 2015 Jan 28;5(1):3. doi: 10.1186/s13601-015-0047-x. eCollection 2015.


Generation of β-lactoglobulin-modified transgenic goats by homologous recombination.

Zhu H, Hu L, Liu J, Chen H, Cui C, Song Y, Jin Y, Zhang Y.

FEBS J. 2016 Dec;283(24):4600-4613. doi: 10.1111/febs.13950. Epub 2016 Dec 5.


Gene targeting by TALEN-induced homologous recombination in goats directs production of β-lactoglobulin-free, high-human lactoferrin milk.

Cui C, Song Y, Liu J, Ge H, Li Q, Huang H, Hu L, Zhu H, Jin Y, Zhang Y.

Sci Rep. 2015 May 21;5:10482. doi: 10.1038/srep10482.


Generation of beta-lactoglobulin knock-out goats using CRISPR/Cas9.

Zhou W, Wan Y, Guo R, Deng M, Deng K, Wang Z, Zhang Y, Wang F.

PLoS One. 2017 Oct 10;12(10):e0186056. doi: 10.1371/journal.pone.0186056. eCollection 2017.


Targeted microRNA expression in dairy cattle directs production of β-lactoglobulin-free, high-casein milk.

Jabed A, Wagner S, McCracken J, Wells DN, Laible G.

Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16811-6. doi: 10.1073/pnas.1210057109. Epub 2012 Oct 1. Erratum in: Proc Natl Acad Sci U S A. 2016 Dec 5;:.


Efficient introgression of allelic variants by embryo-mediated editing of the bovine genome.

Wei J, Wagner S, Lu D, Maclean P, Carlson DF, Fahrenkrug SC, Laible G.

Sci Rep. 2015 Jul 9;5:11735. doi: 10.1038/srep11735.


The growth and reproduction performance of TALEN-mediated β-lactoglobulin-knockout bucks.

Ge H, Cui C, Liu J, Luo Y, Quan F, Jin Y, Zhang Y.

Transgenic Res. 2016 Oct;25(5):721-9. doi: 10.1007/s11248-016-9967-z. Epub 2016 Jun 7.


Selection of recombinant IgE antibodies binding the beta-lactoglobulin allergen in a conformation-dependent manner.

Jylhä S, Mäkinen-Kiljunen S, Haahtela T, Söderlund H, Takkinen K, Laukkanen ML.

J Immunol Methods. 2009 Oct 31;350(1-2):63-70. doi: 10.1016/j.jim.2009.07.007. Epub 2009 Jul 30.


Allergy therapy by intranasal administration with recombinant Lactococcus lactis Producing bovine beta-lactoglobulin.

Cortes-Perez NG, Ah-Leung S, Bermúdez-Humarán LG, Corthier G, Langella P, Wal JM, Adel-Patient K.

Int Arch Allergy Immunol. 2009;150(1):25-31. doi: 10.1159/000210377. Epub 2009 Apr 2.


Genetic immunisation with bovine beta-lactoglobulin cDNA induces a preventive and persistent inhibition of specific anti-BLG IgE response in mice.

Adel-Patient K, Creminon C, Boquet D, Wal JM, Chatel JM.

Int Arch Allergy Immunol. 2001 Sep;126(1):59-67.


Generation of TALE nickase-mediated gene-targeted cows expressing human serum albumin in mammary glands.

Luo Y, Wang Y, Liu J, Cui C, Wu Y, Lan H, Chen Q, Liu X, Quan F, Guo Z, Zhang Y.

Sci Rep. 2016 Feb 8;6:20657. doi: 10.1038/srep20657.


Detection of β-lactoglobulin in human breast-milk 7 days after cow milk ingestion.

Matangkasombut P, Padungpak S, Thaloengsok S, Kamchaisatian W, Sasisakulporn C, Jotikasthira W, Benjaponpitak S, Manuyakorn W.

Paediatr Int Child Health. 2017 Aug;37(3):199-203. doi: 10.1080/20469047.2017.1289310. Epub 2017 Feb 22.


Expression, purification and characterization of zinc-finger nuclease to knockout the goat beta-lactoglobulin gene.

Song Y, Cui C, Zhu H, Li Q, Zhao F, Jin Y.

Protein Expr Purif. 2015 Aug;112:1-7. doi: 10.1016/j.pep.2015.04.004. Epub 2015 Apr 15.


Genome edited sheep and cattle.

Proudfoot C, Carlson DF, Huddart R, Long CR, Pryor JH, King TJ, Lillico SG, Mileham AJ, McLaren DG, Whitelaw CB, Fahrenkrug SC.

Transgenic Res. 2015 Feb;24(1):147-53. doi: 10.1007/s11248-014-9832-x. Epub 2014 Sep 10.

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