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Items: 1 to 20 of 86

1.

Insights into the prevalence and underlying causes of clonal variation through transcriptomic analysis in Pichia pastoris.

Aw R, Barton GR, Leak DJ.

Appl Microbiol Biotechnol. 2017 Jun;101(12):5045-5058. doi: 10.1007/s00253-017-8317-2. Epub 2017 May 22.

2.

Effect of Plasmid Design and Type of Integration Event on Recombinant Protein Expression in Pichia pastoris.

Vogl T, Gebbie L, Palfreyman RW, Speight R.

Appl Environ Microbiol. 2018 Mar 1;84(6). pii: e02712-17. doi: 10.1128/AEM.02712-17. Print 2018 Mar 15.

3.

Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris.

Nordén K, Agemark M, Danielson JÅ, Alexandersson E, Kjellbom P, Johanson U.

BMC Biotechnol. 2011 May 10;11:47. doi: 10.1186/1472-6750-11-47.

4.

Quantitative iTRAQ LC-MS/MS proteomics reveals the cellular response to heterologous protein overexpression and the regulation of HAC1 in Pichia pastoris.

Lin XQ, Liang SL, Han SY, Zheng SP, Ye YR, Lin Y.

J Proteomics. 2013 Oct 8;91:58-72. doi: 10.1016/j.jprot.2013.06.031. Epub 2013 Jul 11.

PMID:
23851310
5.

Comparative genomics and transcriptomics of Pichia pastoris.

Love KR, Shah KA, Whittaker CA, Wu J, Bartlett MC, Ma D, Leeson RL, Priest M, Borowsky J, Young SK, Love JC.

BMC Genomics. 2016 Aug 5;17:550. doi: 10.1186/s12864-016-2876-y. Erratum in: BMC Genomics. 2016 Sep 28;17(1):762.

6.

Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris.

Hohenblum H, Gasser B, Maurer M, Borth N, Mattanovich D.

Biotechnol Bioeng. 2004 Feb 20;85(4):367-75.

PMID:
14755554
7.

Enhancement of lipase r27RCL production in Pichia pastoris by regulating gene dosage and co-expression with chaperone protein disulfide isomerase.

Sha C, Yu XW, Lin NX, Zhang M, Xu Y.

Enzyme Microb Technol. 2013 Dec 10;53(6-7):438-43. doi: 10.1016/j.enzmictec.2013.09.009. Epub 2013 Sep 30.

PMID:
24315648
8.

Understanding the effect of foreign gene dosage on the physiology of Pichia pastoris by transcriptional analysis of key genes.

Zhu T, Guo M, Zhuang Y, Chu J, Zhang S.

Appl Microbiol Biotechnol. 2011 Feb;89(4):1127-35. doi: 10.1007/s00253-010-2944-1. Epub 2010 Oct 28.

PMID:
20981418
9.

Influence of copy number on the expression levels of pandemic influenza hemagglutinin recombinant protein in methylotrophic yeast Pichia pastoris.

Athmaram TN, Saraswat S, Singh AK, Rao MK, Gopalan N, Suryanarayana VV, Rao PV.

Virus Genes. 2012 Dec;45(3):440-51. doi: 10.1007/s11262-012-0809-7. Epub 2012 Sep 2.

PMID:
22940846
10.

Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus.

Marx H, Mecklenbräuker A, Gasser B, Sauer M, Mattanovich D.

FEMS Yeast Res. 2009 Dec;9(8):1260-70. doi: 10.1111/j.1567-1364.2009.00561.x. Epub 2009 Aug 6.

11.

Studies to analyse the relationship between IFNα2b gene dosage and its expression, using a Pichia pastoris-based expression system.

Khan MA, Hassan N, Ahmad N, Khan MI, Zafar AU, Khan F, Husnain T.

Yeast. 2014 Jan;31(1):13-28. doi: 10.1002/yea.2990. Epub 2013 Dec 17.

12.

The effect of gene copy number and co-expression of chaperone on production of albumin fusion proteins in Pichia pastoris.

Shen Q, Wu M, Wang HB, Naranmandura H, Chen SQ.

Appl Microbiol Biotechnol. 2012 Nov;96(3):763-72. doi: 10.1007/s00253-012-4337-0. Epub 2012 Aug 12. Erratum in: Appl Microbiol Biotechnol. 2012 Nov;96(3):851. Wu, Ming [corrected to Wu, Min].

PMID:
22885695
13.

Droplet digital PCR-aided screening and characterization of Pichia pastoris multiple gene copy strains.

Cámara E, Albiol J, Ferrer P.

Biotechnol Bioeng. 2016 Jul;113(7):1542-51. doi: 10.1002/bit.25916. Epub 2016 Mar 16.

PMID:
26704939
14.

High-level expression and characterization of a novel serine protease in Pichia pastoris by multi-copy integration.

Shu M, Shen W, Yang S, Wang X, Wang F, Wang Y, Ma L.

Enzyme Microb Technol. 2016 Oct;92:56-66. doi: 10.1016/j.enzmictec.2016.06.007. Epub 2016 Jun 14.

PMID:
27542745
15.

Maximizing recombinant human serum albumin production in a Mut(s) Pichia pastoris strain.

Mallem M, Warburton S, Li F, Shandil I, Nylen A, Kim S, Jiang Y, Meehl M, d'Anjou M, Stadheim TA, Choi BK.

Biotechnol Prog. 2014 Nov-Dec;30(6):1488-96. doi: 10.1002/btpr.1990. Epub 2014 Sep 16.

PMID:
25196297
16.

Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris.

Prielhofer R, Maurer M, Klein J, Wenger J, Kiziak C, Gasser B, Mattanovich D.

Microb Cell Fact. 2013 Jan 24;12:5. doi: 10.1186/1475-2859-12-5.

17.

In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response.

Rebnegger C, Graf AB, Valli M, Steiger MG, Gasser B, Maurer M, Mattanovich D.

Biotechnol J. 2014 Apr;9(4):511-25. doi: 10.1002/biot.201300334. Epub 2014 Jan 14.

18.

Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications.

Camattari A, Goh A, Yip LY, Tan AH, Ng SW, Tran A, Liu G, Liachko I, Dunham MJ, Rancati G.

Microb Cell Fact. 2016 Aug 11;15(1):139. doi: 10.1186/s12934-016-0540-5.

19.

Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions.

Gasser B, Maurer M, Rautio J, Sauer M, Bhattacharyya A, Saloheimo M, Penttilä M, Mattanovich D.

BMC Genomics. 2007 Jun 19;8:179.

20.

A multi-level study of recombinant Pichia pastoris in different oxygen conditions.

Baumann K, Carnicer M, Dragosits M, Graf AB, Stadlmann J, Jouhten P, Maaheimo H, Gasser B, Albiol J, Mattanovich D, Ferrer P.

BMC Syst Biol. 2010 Oct 22;4:141. doi: 10.1186/1752-0509-4-141.

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