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Items: 1 to 20 of 100

1.

Influence of washing and quenching in profiling the metabolome of adherent mammalian cells: a case study with the metastatic breast cancer cell line MDA-MB-231.

Kapoore RV, Coyle R, Staton CA, Brown NJ, Vaidyanathan S.

Analyst. 2017 Jun 7;142(11):2038-2049. doi: 10.1039/c7an00207f. Epub 2017 May 12.

2.

Evaluation of sampling and extraction methodologies for the global metabolic profiling of Saccharophagus degradans.

Shin MH, Lee DY, Liu KH, Fiehn O, Kim KH.

Anal Chem. 2010 Aug 1;82(15):6660-6. doi: 10.1021/ac1012656.

PMID:
20669998
3.

Extending metabolome coverage for untargeted metabolite profiling of adherent cultured hepatic cells.

García-Cañaveras JC, López S, Castell JV, Donato MT, Lahoz A.

Anal Bioanal Chem. 2016 Feb;408(4):1217-30. doi: 10.1007/s00216-015-9227-8. Epub 2016 Jan 14.

PMID:
26769129
4.

Optimizing high-throughput metabolomic biomarker screening: a study of quenching solutions to freeze intracellular metabolism in CHO cells.

Kronthaler J, Gstraunthaler G, Heel C.

OMICS. 2012 Mar;16(3):90-7. doi: 10.1089/omi.2011.0048.

PMID:
22401654
5.

Translational Metabolomics of Head Injury: Exploring Dysfunctional Cerebral Metabolism with Ex Vivo NMR Spectroscopy-Based Metabolite Quantification.

Wolahan SM, Hirt D, Glenn TC.

In: Kobeissy FH, editor. Brain Neurotrauma: Molecular, Neuropsychological, and Rehabilitation Aspects. Boca Raton (FL): CRC Press/Taylor & Francis; 2015. Chapter 25.

6.

Evaluation and optimization of metabolome sample preparation methods for Saccharomyces cerevisiae.

Kim S, Lee DY, Wohlgemuth G, Park HS, Fiehn O, Kim KH.

Anal Chem. 2013 Feb 19;85(4):2169-76. doi: 10.1021/ac302881e. Epub 2013 Jan 25.

PMID:
23289506
7.

The development of metabolomic sampling procedures for Pichia pastoris, and baseline metabolome data.

Tredwell GD, Edwards-Jones B, Leak DJ, Bundy JG.

PLoS One. 2011 Jan 21;6(1):e16286. doi: 10.1371/journal.pone.0016286.

8.

Reducing time and increasing sensitivity in sample preparation for adherent mammalian cell metabolomics.

Lorenz MA, Burant CF, Kennedy RT.

Anal Chem. 2011 May 1;83(9):3406-14. doi: 10.1021/ac103313x. Epub 2011 Apr 1.

9.

Sample preparation for the metabolomics investigation of poly-gamma-glutamate-producing Bacillus licheniformis by GC-MS.

Li X, Long D, Ji J, Yang W, Zeng Z, Guo S, Ji Z, Qi G, Chen S.

J Microbiol Methods. 2013 Jul;94(1):61-7. doi: 10.1016/j.mimet.2013.04.006. Epub 2013 Apr 28.

PMID:
23628234
10.

Extraction parameters for metabolomics from cultured cells.

Ser Z, Liu X, Tang NN, Locasale JW.

Anal Biochem. 2015 Apr 15;475:22-8. doi: 10.1016/j.ab.2015.01.003. Epub 2015 Jan 19.

11.

Towards quantitative metabolomics of mammalian cells: development of a metabolite extraction protocol.

Dietmair S, Timmins NE, Gray PP, Nielsen LK, Krömer JO.

Anal Biochem. 2010 Sep 15;404(2):155-64. doi: 10.1016/j.ab.2010.04.031. Epub 2010 May 21.

PMID:
20435011
12.

Fast filtration sampling protocol for mammalian suspension cells tailored for phosphometabolome profiling by capillary ion chromatography - tandem mass spectrometry.

Kvitvang HF, Bruheim P.

J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Aug 15;998-999:45-9. doi: 10.1016/j.jchromb.2015.06.018. Epub 2015 Jun 20.

PMID:
26151192
13.

Strategy for choosing extraction procedures for NMR-based metabolomic analysis of mammalian cells.

Martineau E, Tea I, Loaëc G, Giraudeau P, Akoka S.

Anal Bioanal Chem. 2011 Oct;401(7):2133-42. doi: 10.1007/s00216-011-5310-y. Epub 2011 Aug 13.

PMID:
21837464
14.

Metabolomics of Pichia pastoris: impact of buffering conditions on the kinetics and nature of metabolite loss during quenching.

Mattanovich M, Russmayer H, Scharl-Hirsch T, Puxbaum V, Burgard J, Mattanovich D, Hann S.

FEMS Yeast Res. 2017 May 1;17(3). doi: 10.1093/femsyr/fox016.

PMID:
28334329
15.

Analysis of Intracellular Metabolites from Microorganisms: Quenching and Extraction Protocols.

Pinu FR, Villas-Boas SG, Aggio R.

Metabolites. 2017 Oct 23;7(4). pii: E53. doi: 10.3390/metabo7040053. Review.

16.

Analytical methodology for metabolomics study of adherent mammalian cells using NMR, GC-MS and LC-HRMS.

Madji Hounoum B, Blasco H, Nadal-Desbarats L, Diémé B, Montigny F, Andres CR, Emond P, Mavel S.

Anal Bioanal Chem. 2015 Nov;407(29):8861-72. doi: 10.1007/s00216-015-9047-x. Epub 2015 Oct 7.

PMID:
26446897
17.

Fast sampling method for mammalian cell metabolic analyses using liquid chromatography-mass spectrometry.

Martano G, Delmotte N, Kiefer P, Christen P, Kentner D, Bumann D, Vorholt JA.

Nat Protoc. 2015 Jan;10(1):1-11. doi: 10.1038/nprot.2014.198. Epub 2014 Dec 4.

PMID:
25474028
18.

Development of quenching and washing protocols for quantitative intracellular metabolite analysis of uninfected and baculovirus-infected insect cells.

Tran TT, Dietmair S, Chan LC, Huynh HT, Nielsen LK, Reid S.

Methods. 2012 Mar;56(3):396-407. doi: 10.1016/j.ymeth.2011.11.009. Epub 2011 Dec 7.

PMID:
22166686
19.

Towards an unbiased metabolic profiling of protozoan parasites: optimisation of a Leishmania sampling protocol for HILIC-orbitrap analysis.

t'Kindt R, Jankevics A, Scheltema RA, Zheng L, Watson DG, Dujardin JC, Breitling R, Coombs GH, Decuypere S.

Anal Bioanal Chem. 2010 Nov;398(5):2059-69. doi: 10.1007/s00216-010-4139-0. Epub 2010 Sep 8. Erratum in: Anal Bioanal Chem. 2011 Apr;400(2):635.

PMID:
20824428
20.

Optimization of harvesting, extraction, and analytical protocols for UPLC-ESI-MS-based metabolomic analysis of adherent mammalian cancer cells.

Bi H, Krausz KW, Manna SK, Li F, Johnson CH, Gonzalez FJ.

Anal Bioanal Chem. 2013 Jun;405(15):5279-89. doi: 10.1007/s00216-013-6927-9. Epub 2013 Apr 19.

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