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27715478,26972786,29401748,26163061,19717337,27248786,23662585,28872832,26678795,27173394,33233573,26607475,27012179,29144411,26873572,31591283,28146093,29186905,30193856,34756455,30378352,19450981,26642994,28513534,25325213,29198584,26538121,28437710,31482458,29186809,23563807,33528997,25950603,17312962,30244135,33808496,35320268,25728760,15971588,25237853,17201061,21300154,27236559,21955150,17283213,36551109,29170966,29626546,23811206,29132385,17627883,26383776,30244458,30866536,20692149,33096353,24895227,23403083,19051203,26768582,21838689,19377982,32933142,33146506,32087127,27790993,35416400,23851587,26389865,25791604,18571753,25924917,17017882,27613616,18770826,18776325,24044051,28255887,25708387,25912679,20582587,25277749,20153201,17072603,21838693,26980177,31560515,21627466,33039563,20677985,28061688,35209816,34649356,26133761,36430951,28325909,19319884,19377986,28275062,24045702,22261866,22326915,22860007,19684896,22491855,26603636,30707214,18029757,29700486,22480209,30830295,21492073,24269231,23405949,26293334,35700936,17681489,36574475,29897179,32234785,32813254,34204006,31437572,35274201,27043498,21838692,19202068,21342116,26344234,34323642,16787315,31664817,27155227,22689764,29054799,29926454,10943570,29301363,29039727,31200538,25050359,28265062,29431588,17626559,33086095,32732393,31010064,21793789,35151405,23216233,35130439,27173731,26473828,31196363,32316469,20541015,28938065,30156834,31099939,30957848,30746785,19751057,27243551,22536456,25992428,33559947,24415766,30968117,29524380,28916151,28544018,31040350,33151990,28884330,31861277,25849197,25866271,19075955,21534918,16594622,25269812,25430795,27893356,31196711,19384268,31689200,29297663,32311376,31392985,35026382,24404332,34500620,27637356,28582756,32842557,15461575,27898020,24682699,30609709,28721653,20729797,21712014,25866269,32490558,17627582,29283416,16081419,32582641,26476448,32282107,32527457,31990606,19860714,31640176,36583362,26757406,21182455,35671695,31111537,31912723,36156773,28961063,31036165,22352726,35889390,29872716,28300769,27467247,28092358,31939181,29245156,24415767,14576330,25866268,28407231,21848510,33269185,33074117,22951893,29054802,25057429,18971322,22487172,32375354,25866280,30472954,24772298,25866270,29505267,19061867,22380008,30929239,26972787,15333917,25490402,33671039,31341361,32764266,27335672,27242994,34137415,22719856,31774997,26000380,19458497,27713328,23139410,28594360,36037714,19702567,32700427,25295268,29050945,27807347,21420292,30517682,23130020,32659966,20641927,19465396,27873915,20565094,34638764,31900238,28767098,36693249,36282114,29414854,31141341,28635657,18187867,28696509,30085205,31037641,30912676,28969551,32758252,32659994,29574128,29850750,19417060,23745560,35870338,30714390,25229067,30321620,21441582,27180958,25093706,32990095,30125604,25723512,29770378,29630917,16231083,26996618,17251551,33875130,23515081,30633241,32048971,25560701,34170922,32991255,29425173,31255977,20592747,24083239,29868605,36434145,10408383,29913134,20049590,28962871,27258484,23090891,27200340,14527514,30340426,27934108,32631977,29746951,32585627,28536411,30114357,31702902,33920628,16771507,28837119,35052385,30326601,28805744,31333603,33679681,36910146,21080135,36558056,24748197,32627415,34148284,28117671,36544906,36574492,37024672,21359096,15629041,34589111,28373101,22943666,30727784,28088878,31638376,17464274,29548926,35673581,30561987,30563044,28609610,29927117,23402035,34013167,33536494,23042245,33142793,33123281,34739228,31950158,28730439,23563318,32363852,30773785,11884639,27763826,10657289,23094139,25291143,30150587,37068137,35681520,30534748,28009914,7519769,30128033,30125606,21838684,30212189,29104137,26153774,20714306,33473329,30926532,30642129,31824168,25913927,19029139,28635257,30411046,10539988,29788485,21904667,23154273,10995196,28945147,35993818,34509496,33988982,28841163,31260780,20159999,34664030,31801185,28069377,20660786,18594898,10671648,33035750,34447741,11590140,9667843,35025411,18311508,37149607,11539574,21838691,23401749,27648925,29054800,35025402,34233000,30015643,29499933,34010626,32463617,29065503,21838690,31778956,28433327,28692589,29981799,31881936,30556364,34854680,33822453,10943568,32287535,27911494,27999357,29080264,21413174,20870801,36410759,33038031,36987838,36733700,35351813,29964079,34467709,29745541,36188221,31169327,12903347,19707307,23316203,32920262,9238640,33917467,27581942,32098923,35668324,11716673,34078451,29224849,31332450,12009452,36431072,23299833,30149664,33251048,31460550,16202220,33494545,30487456,37090419,28703779,31362673,29928493,35056696,28110747,30583324,28084479,34778766,31597871,31906837,24281560,22649701,24373999,34095130,28054050,30986697,8804830,30631065,24455181,34659704,33688891,32270033,30029472,28218835,35625815,30905751,36393853,11764782,31961667,29568464,30458067,12297271,26812623,36312558,15769669,36358889,36524831,24276022,33206450,32548090,22347221,29851335,30586898,32549920,1721092,20645365,30274155,31050104,25236655,30931575,28850067,30384431,37139427,2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In vitro evolution of chemically-modified nucleic acid aptamers: Pros and cons, and comprehensive selection strategies.

Lipi F, Chen S, Chakravarthy M, Rakesh S, Veedu RN. Lipi F, et al. RNA Biol. 2016 Dec;13(12):1232-1245. doi: 10.1080/15476286.2016.1236173. Epub 2016 Oct 7. RNA Biol. 2016. PMID: 27715478 Free PMC article. Review.

In vitro RNA SELEX for the generation of chemically-optimized therapeutic RNA drugs.

Urak KT, Shore S, Rockey WM, Chen SJ, McCaffrey AP, Giangrande PH. Urak KT, et al. Methods. 2016 Jul 1;103:167-74. doi: 10.1016/j.ymeth.2016.03.003. Epub 2016 Mar 10. Methods. 2016. PMID: 26972786 Free PMC article.

Key Aspects of Nucleic Acid Library Design for in Vitro Selection.

Vorobyeva MA, Davydova AS, Vorobjev PE, Venyaminova AG. Vorobyeva MA, et al. Int J Mol Sci. 2018 Feb 5;19(2):470. doi: 10.3390/ijms19020470. Int J Mol Sci. 2018. PMID: 29401748 Free PMC article. Review.

Characterisation of aptamer-target interactions by branched selection and high-throughput sequencing of SELEX pools.

Dupont DM, Larsen N, Jensen JK, Andreasen PA, Kjems J. Dupont DM, et al. Nucleic Acids Res. 2015 Dec 2;43(21):e139. doi: 10.1093/nar/gkv700. Epub 2015 Jul 10. Nucleic Acids Res. 2015. PMID: 26163061 Free PMC article.

Aptamers: a new class of oligonucleotides in the drug discovery pipeline?

Dausse E, Da Rocha Gomes S, Toulmé JJ. Dausse E, et al. Curr Opin Pharmacol. 2009 Oct;9(5):602-7. doi: 10.1016/j.coph.2009.07.006. Epub 2009 Aug 28. Curr Opin Pharmacol. 2009. PMID: 19717337 Review.

Selection of Natural and Base-Modified DNA Aptamers for a Camptothecin Derivative.

Fujita H, Kuwahara M. Fujita H, et al. Curr Protoc Nucleic Acid Chem. 2016 Jun 1;65:9.10.1-9.10.19. doi: 10.1002/cpnc.5. Curr Protoc Nucleic Acid Chem. 2016. PMID: 27248786

Capillary electrophoresis-systematic evolution of ligands by exponential enrichment selection of base- and sugar-modified DNA aptamers: target binding dominated by 2'-O,4'-C-methylene-bridged/locked nucleic acid primer.

Kasahara Y, Irisawa Y, Fujita H, Yahara A, Ozaki H, Obika S, Kuwahara M. Kasahara Y, et al. Anal Chem. 2013 May 21;85(10):4961-7. doi: 10.1021/ac400058z. Epub 2013 May 10. Anal Chem. 2013. PMID: 23662585

Chemically Modified, α-Amino-3-hydroxy-5-methyl-4-isoxazole (AMPA) Receptor RNA Aptamers Designed for in Vivo Use.

Huang Z, Wen W, Wu A, Niu L. Huang Z, et al. ACS Chem Neurosci. 2017 Nov 15;8(11):2437-2445. doi: 10.1021/acschemneuro.7b00211. Epub 2017 Sep 5. ACS Chem Neurosci. 2017. PMID: 28872832

An improved SELEX technique for selection of DNA aptamers binding to M-type 11 of Streptococcus pyogenes.

Hamula CL, Peng H, Wang Z, Tyrrell GJ, Li XF, Le XC. Hamula CL, et al. Methods. 2016 Mar 15;97:51-7. doi: 10.1016/j.ymeth.2015.12.005. Epub 2015 Dec 8. Methods. 2016. PMID: 26678795

Post-SELEX optimization of aptamers.

Gao S, Zheng X, Jiao B, Wang L. Gao S, et al. Anal Bioanal Chem. 2016 Jul;408(17):4567-73. doi: 10.1007/s00216-016-9556-2. Epub 2016 May 12. Anal Bioanal Chem. 2016. PMID: 27173394 Review.

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