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Items: 1 to 20 of 114

1.

MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering.

Kostyrko K, Neuenschwander S, Junier T, Regamey A, Iseli C, Schmid-Siegert E, Bosshard S, Majocchi S, Le Fourn V, Girod PA, Xenarios I, Mermod N.

Biotechnol Bioeng. 2017 Feb;114(2):384-396. doi: 10.1002/bit.26086. Epub 2016 Oct 3.

2.

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids.

Sakuma T, Takenaga M, Kawabe Y, Nakamura T, Kamihira M, Yamamoto T.

Int J Mol Sci. 2015 Oct 9;16(10):23849-66. doi: 10.3390/ijms161023849.

3.

A short synthetic chimeric sequence harboring matrix attachment region/PSAR2 increases transgene expression in Chinese hamster ovary cells.

Li Q, Wang W, Guo X, Jia YL, Wang YF, Wang TY.

Biosci Biotechnol Biochem. 2017 Sep;81(9):1755-1761. doi: 10.1080/09168451.2017.1350563. Epub 2017 Jul 17.

PMID:
28715274
4.

Molecular characterization of a human matrix attachment region that improves transgene expression in CHO cells.

Sun QL, Zhao CP, Chen SN, Wang L, Wang TY.

Gene. 2016 May 15;582(2):168-72. doi: 10.1016/j.gene.2016.02.009. Epub 2016 Feb 9.

PMID:
26869318
5.

Assays for DNA double-strand break repair by microhomology-based end-joining repair mechanisms.

Kostyrko K, Mermod N.

Nucleic Acids Res. 2016 Apr 7;44(6):e56. doi: 10.1093/nar/gkv1349. Epub 2015 Dec 10.

6.

Use of the chicken lysozyme 5' matrix attachment region to generate high producer CHO cell lines.

Girod PA, Zahn-Zabal M, Mermod N.

Biotechnol Bioeng. 2005 Jul 5;91(1):1-11.

PMID:
15889435
7.

Scaffold/matrix attachment regions from CHO cell chromosome enhanced the stable transfection efficiency and the expression of transgene in CHO cells.

Chang M, Liu R, Jin Q, Liu Y, Wang X.

Biotechnol Appl Biochem. 2014 Sep-Oct;61(5):510-6. doi: 10.1002/bab.1204. Epub 2014 Jun 12.

PMID:
24397363
8.

[Effect of Intron Orientation on the Expression of Transgene Imposed by MAR Expression Vector in Stably Recombinant CHO Cells].

Li Q, Zhao CP, Wang XY, Sun QL, Wang TY.

Sichuan Da Xue Xue Bao Yi Xue Ban. 2016 Mar;47(2):189-91, 243. Chinese.

PMID:
27263292
9.

Generation of stable cell lines by site-specific integration of transgenes into engineered Chinese hamster ovary strains using an FLP-FRT system.

Zhou H, Liu ZG, Sun ZW, Huang Y, Yu WY.

J Biotechnol. 2010 May 17;147(2):122-9. doi: 10.1016/j.jbiotec.2010.03.020. Epub 2010 Apr 3.

PMID:
20371256
10.

MAR elements and transposons for improved transgene integration and expression.

Ley D, Harraghy N, Le Fourn V, Bire S, Girod PA, Regamey A, Rouleux-Bonnin F, Bigot Y, Mermod N.

PLoS One. 2013 Apr 30;8(4):e62784. doi: 10.1371/journal.pone.0062784. Print 2013.

11.

Involvement of Ku80 in microhomology-mediated end joining for DNA double-strand breaks in vivo.

Katsura Y, Sasaki S, Sato M, Yamaoka K, Suzukawa K, Nagasawa T, Yokota J, Kohno T.

DNA Repair (Amst). 2007 May 1;6(5):639-48. Epub 2007 Jan 22.

PMID:
17236818
12.

Epigenetic regulatory elements: Recent advances in understanding their mode of action and use for recombinant protein production in mammalian cells.

Harraghy N, Calabrese D, Fisch I, Girod PA, LeFourn V, Regamey A, Mermod N.

Biotechnol J. 2015 Jul;10(7):967-78. doi: 10.1002/biot.201400649. Epub 2015 Jun 23. Review.

PMID:
26099730
13.

High-level transgene expression by homologous recombination-mediated gene transfer.

Grandjean M, Girod PA, Calabrese D, Kostyrko K, Wicht M, Yerly F, Mazza C, Beckmann JS, Martinet D, Mermod N.

Nucleic Acids Res. 2011 Aug;39(15):e104. doi: 10.1093/nar/gkr436. Epub 2011 Jun 7.

14.

Improved recombinant antibody production by CHO cells using a production enhancer DNA element with repeated transgene integration at a predetermined chromosomal site.

Kawabe Y, Inao T, Komatsu S, Huang G, Ito A, Omasa T, Kamihira M.

J Biosci Bioeng. 2017 Mar;123(3):390-397. doi: 10.1016/j.jbiosc.2016.10.011. Epub 2016 Nov 14.

PMID:
27856232
15.

Using matrix attachment regions to improve recombinant protein production.

Harraghy N, Buceta M, Regamey A, Girod PA, Mermod N.

Methods Mol Biol. 2012;801:93-110. doi: 10.1007/978-1-61779-352-3_7.

PMID:
21987249
16.

Improved recombinant gene expression in CHO cells using matrix attachment regions.

Kim JM, Kim JS, Park DH, Kang HS, Yoon J, Baek K, Yoon Y.

J Biotechnol. 2004 Jan 22;107(2):95-105.

PMID:
14711493
17.

Improved transgene integration into the Chinese hamster ovary cell genome using the Cre-loxP system.

Inao T, Kawabe Y, Yamashiro T, Kameyama Y, Wang X, Ito A, Kamihira M.

J Biosci Bioeng. 2015 Jul;120(1):99-106. doi: 10.1016/j.jbiosc.2014.11.019. Epub 2015 Jan 22.

PMID:
25617966
18.

Positional effects of the matrix attachment region on transgene expression in stably transfected CHO cells.

Wang TY, Zhang JH, Jing CQ, Yang XJ, Lin JT.

Cell Biol Int. 2010 Feb;34(2):141-5. doi: 10.1042/CBI20090017.

PMID:
19947951
19.

A novel regulatory element (E77) isolated from CHO-K1 genomic DNA enhances stable gene expression in Chinese hamster ovary cells.

Kang SY, Kim YG, Kang S, Lee HW, Lee EG.

Biotechnol J. 2016 May;11(5):633-41. doi: 10.1002/biot.201500464. Epub 2016 Feb 11.

20.

Ubiquitous Chromatin Opening Elements (UCOEs) effect on transgene position and expression stability in CHO cells following methotrexate (MTX) amplification.

Betts Z, Dickson AJ.

Biotechnol J. 2016 Mar;11(4):554-64. doi: 10.1002/biot.201500159. Epub 2016 Jan 19.

PMID:
26632501

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