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A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA- alkyl-transferase by protein engineering.

Vettone A, Serpe M, Hidalgo A, Berenguer J, del Monaco G, Valenti A, Rossi M, Ciaramella M, Perugino G.

Extremophiles. 2016 Jan;20(1):1-13. doi: 10.1007/s00792-015-0791-9. Erratum in: Extremophiles. 2016 Jan;20(1):15-7.


Structure-function relationships governing activity and stability of a DNA alkylation damage repair thermostable protein.

Perugino G, Miggiano R, Serpe M, Vettone A, Valenti A, Lahiri S, Rossi F, Rossi M, Rizzi M, Ciaramella M.

Nucleic Acids Res. 2015 Oct 15;43(18):8801-16. doi: 10.1093/nar/gkv774. Epub 2015 Jul 30.


Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with a fluorescent probe.

Rossi F, Morrone C, Massarotti A, Ferraris DM, Valenti A, Perugino G, Miggiano R.

Biochem Biophys Res Commun. 2018 Jun 7;500(3):698-703. doi: 10.1016/j.bbrc.2018.04.139. Epub 2018 Apr 30.


Activity and regulation of archaeal DNA alkyltransferase: conserved protein involved in repair of DNA alkylation damage.

Perugino G, Vettone A, Illiano G, Valenti A, Ferrara MC, Rossi M, Ciaramella M.

J Biol Chem. 2012 Feb 3;287(6):4222-31. doi: 10.1074/jbc.M111.308320. Epub 2011 Dec 13. Erratum in: J Biol Chem. 2015 Jan 9;290(2):885.


In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag.

Visone V, Han W, Perugino G, Del Monaco G, She Q, Rossi M, Valenti A, Ciaramella M.

PLoS One. 2017 Oct 3;12(10):e0185791. doi: 10.1371/journal.pone.0185791. eCollection 2017.


The Sulfolobus solfataricus electron donor partners of thermophilic CYP119: an unusual non-NAD(P)H-dependent cytochrome P450 system.

Puchkaev AV, Ortiz de Montellano PR.

Arch Biochem Biophys. 2005 Feb 1;434(1):169-77.


A highly acid-stable and thermostable endo-beta-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus.

Huang Y, Krauss G, Cottaz S, Driguez H, Lipps G.

Biochem J. 2005 Jan 15;385(Pt 2):581-8.


Engineering the allosteric properties of archaeal non-phosphorylating glyceraldehyde-3-phosphate dehydrogenases.

Ito F, Miyake M, Fushinobu S, Nakamura S, Shimizu K, Wakagi T.

Biochim Biophys Acta. 2014 Apr;1844(4):759-66. doi: 10.1016/j.bbapap.2014.01.017. Epub 2014 Jan 31.


Importance of a potential salt bridge and hydrophobic core in the function and oligomerization of a small heat shock protein.

Wen Z, Wang Y, Xu X, Yang B, Li W, Xie M.

Protein Pept Lett. 2010 Jun;17(6):751-8.


In Vivo Formation of the Protein Disulfide Bond That Enhances the Thermostability of Diphosphomevalonate Decarboxylase, an Intracellular Enzyme from the Hyperthermophilic Archaeon Sulfolobus solfataricus.

Hattori A, Unno H, Goda S, Motoyama K, Yoshimura T, Hemmi H.

J Bacteriol. 2015 Nov;197(21):3463-71. doi: 10.1128/JB.00352-15. Epub 2015 Aug 24.


A novel thermostable arylesterase from the archaeon Sulfolobus solfataricus P1: purification, characterization, and expression.

Park YJ, Yoon SJ, Lee HB.

J Bacteriol. 2008 Dec;190(24):8086-95. doi: 10.1128/JB.00803-08. Epub 2008 Oct 17.


Structural basis of the destabilization produced by an amino-terminal tag in the beta-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus.

Ausili A, Cobucci-Ponzano B, Di Lauro B, D'Avino R, Scirè A, Rossi M, Tanfani F, Moracci M.

Biochimie. 2006 Jul;88(7):807-17. Epub 2006 Feb 9.


High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers.

Restaino OF, Borzacchiello MG, Scognamiglio I, Fedele L, Alfano A, Porzio E, Manco G, De Rosa M, Schiraldi C.

BMC Biotechnol. 2018 Mar 20;18(1):18. doi: 10.1186/s12896-018-0427-0.


Effect of N2-guanyl modifications on early steps in catalysis of polymerization by Sulfolobus solfataricus P2 DNA polymerase Dpo4 T239W.

Zhang H, Guengerich FP.

J Mol Biol. 2010 Feb 5;395(5):1007-18. doi: 10.1016/j.jmb.2009.11.071. Epub 2009 Dec 4.


Novel medium-chain prenyl diphosphate synthase from the thermoacidophilic archaeon Sulfolobus solfataricus.

Hemmi H, Ikejiri S, Yamashita S, Nishino T.

J Bacteriol. 2002 Feb;184(3):615-20.


Identification and functional verification of archaeal-type phosphoenolpyruvate carboxylase, a missing link in archaeal central carbohydrate metabolism.

Ettema TJ, Makarova KS, Jellema GL, Gierman HJ, Koonin EV, Huynen MA, de Vos WM, van der Oost J.

J Bacteriol. 2004 Nov;186(22):7754-62.

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