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Items: 1 to 20 of 133

1.

High-efficiency scarless genetic modification in Escherichia coli by using lambda red recombination and I-SceI cleavage.

Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu J, Jiang W, Yang S.

Appl Environ Microbiol. 2014 Jul;80(13):3826-34. doi: 10.1128/AEM.00313-14. Epub 2014 Apr 18.

2.

Multiple-site genetic modifications in Escherichia coli using lambda-Red recombination and I-SceI cleavage.

Yang J, Sun B, Huang H, Chen B, Xu C, Wang X, Liu J, Diao L.

Biotechnol Lett. 2015 Oct;37(10):2011-8. doi: 10.1007/s10529-015-1878-1. Epub 2015 Jun 11.

PMID:
26063619
3.

I-SceI-mediated plasmid deletion and intra-molecular recombination in Spiroplasma citri.

Breton M, Duret S, Béven L, Dubrana MP, Renaudin J.

J Microbiol Methods. 2011 Feb;84(2):216-22. doi: 10.1016/j.mimet.2010.11.020. Epub 2010 Dec 1.

PMID:
21129414
4.

Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains.

Lee DJ, Bingle LE, Heurlier K, Pallen MJ, Penn CW, Busby SJ, Hobman JL.

BMC Microbiol. 2009 Dec 9;9:252. doi: 10.1186/1471-2180-9-252.

5.

Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli.

Tischer BK, von Einem J, Kaufer B, Osterrieder N.

Biotechniques. 2006 Feb;40(2):191-7.

6.

[Markerless DNA deletion based on Red recombination and in vivo I-Sec I endonuclease cleavage in Escherichia coli chromosome].

Zhu M, Yu J, Zhou C, Fang H.

Sheng Wu Gong Cheng Xue Bao. 2016 Jan;32(1):114-26. Chinese.

PMID:
27363204
7.

Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration.

Al-Hinai MA, Fast AG, Papoutsakis ET.

Appl Environ Microbiol. 2012 Nov;78(22):8112-21. doi: 10.1128/AEM.02214-12. Epub 2012 Sep 14.

8.

En passant mutagenesis: a two step markerless red recombination system.

Tischer BK, Smith GA, Osterrieder N.

Methods Mol Biol. 2010;634:421-30. doi: 10.1007/978-1-60761-652-8_30.

PMID:
20677001
9.

Scarless chromosomal gene knockout methods.

Sung BH, Lee JH, Kim SC.

Methods Mol Biol. 2011;765:43-54. doi: 10.1007/978-1-61779-197-0_3.

PMID:
21815085
10.

Phage recombinases and their applications.

Murphy KC.

Adv Virus Res. 2012;83:367-414. doi: 10.1016/B978-0-12-394438-2.00008-6. Review.

PMID:
22748814
11.

A PCR-free cloning method for the targeted φ80 Int-mediated integration of any long DNA fragment, bracketed with meganuclease recognition sites, into the Escherichia coli chromosome.

Ublinskaya AA, Samsonov VV, Mashko SV, Stoynova NV.

J Microbiol Methods. 2012 Jun;89(3):167-73. doi: 10.1016/j.mimet.2012.03.013. Epub 2012 Mar 29.

PMID:
22484061
12.

Development of a markerless gene replacement system in Corynebacterium glutamicum using upp as a counter-selection marker.

Ma W, Wang X, Mao Y, Wang Z, Chen T, Zhao X.

Biotechnol Lett. 2015 Mar;37(3):609-17. doi: 10.1007/s10529-014-1718-8. Epub 2014 Nov 7.

PMID:
25376333
14.

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli.

Pyne ME, Moo-Young M, Chung DA, Chou CP.

Appl Environ Microbiol. 2015 Aug;81(15):5103-14. doi: 10.1128/AEM.01248-15. Epub 2015 May 22.

15.

Self-excising integrative yeast plasmid vectors containing an intronated recombinase gene.

Agaphonov M, Alexandrov A.

FEMS Yeast Res. 2014 Nov;14(7):1048-54. doi: 10.1111/1567-1364.12197. Epub 2014 Sep 5.

16.

Application of lambda Red recombination system to Vibrio cholerae genetics: simple methods for inactivation and modification of chromosomal genes.

Yamamoto S, Izumiya H, Morita M, Arakawa E, Watanabe H.

Gene. 2009 Jun 1;438(1-2):57-64. doi: 10.1016/j.gene.2009.02.015. Epub 2009 Mar 5.

PMID:
19268696
17.

An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome.

Wei Y, Deng P, Mohsin A, Yang Y, Zhou H, Guo M, Fang H.

PLoS One. 2017 Oct 24;12(10):e0186891. doi: 10.1371/journal.pone.0186891. eCollection 2017.

18.

PCR-mediated gene replacement in Escherichia coli.

Murphy KC, Campellone KG, Poteete AR.

Gene. 2000 Apr 4;246(1-2):321-30.

PMID:
10767554
19.

Construction and functional characterization of an integrative form lambda Red recombineering Escherichia coli strain.

Song J, Dong H, Ma C, Zhao B, Shang G.

FEMS Microbiol Lett. 2010 Aug 1;309(2):178-83. doi: 10.1111/j.1574-6968.2010.02036.x. Epub 2010 Jun 16.

20.

I-SceI-mediated scarless gene modification via allelic exchange in Clostridium.

Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S.

J Microbiol Methods. 2015 Jan;108:49-60. doi: 10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15.

PMID:
25451462

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