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HIV viral RNA extraction in wax immiscible filtration assisted by surface tension (IFAST) devices.

Berry SM, LaVanway AJ, Pezzi HM, Guckenberger DJ, Anderson MA, Loeb JM, Beebe DJ.

J Mol Diagn. 2014 May;16(3):297-304. doi: 10.1016/j.jmoldx.2014.01.004. Epub 2014 Mar 6.


Automated operation of immiscible filtration assisted by surface tension (IFAST) arrays for streamlined analyte isolation.

Berry SM, Regehr KJ, Casavant BP, Beebe DJ.

J Lab Autom. 2013 Jun;18(3):206-11. doi: 10.1177/2211068212462023. Epub 2012 Sep 26.


One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST).

Berry SM, Alarid ET, Beebe DJ.

Lab Chip. 2011 May 21;11(10):1747-53. doi: 10.1039/c1lc00004g. Epub 2011 Mar 21.


Facile and rapid DNA extraction and purification from food matrices using IFAST (immiscible filtration assisted by surface tension).

Strotman LN, Lin G, Berry SM, Johnson EA, Beebe DJ.

Analyst. 2012 Sep 7;137(17):4023-8. doi: 10.1039/c2an35506j. Epub 2012 Jul 20.


Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients.

Kaur P, Khong WX, Wee SY, Tan EL, Pipper J, Koay E, Ng KY, Yap JK, Chew KK, Tan MT, Leo YS, Inoue M, Ng OT.

PLoS One. 2014 Mar 6;9(3):e89826. doi: 10.1371/journal.pone.0089826. eCollection 2014.


Purification of cell subpopulations via immiscible filtration assisted by surface tension (IFAST).

Berry SM, Strotman LN, Kueck JD, Alarid ET, Beebe DJ.

Biomed Microdevices. 2011 Dec;13(6):1033-42. doi: 10.1007/s10544-011-9573-z.


A RealTime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A-H, group O and group N samples.

Tang N, Huang S, Salituro J, Mak WB, Cloherty G, Johanson J, Li YH, Schneider G, Robinson J, Hackett J Jr, Swanson P, Abravaya K.

J Virol Methods. 2007 Dec;146(1-2):236-45. Epub 2007 Aug 17.


HIV reverse transcriptase activity assay: a feasible surrogate for HIV viral load measurement in China.

Huang D, Zhuang Y, Zhai S, Song Y, Liu Q, Zhao S, Wang S, Li X, Kang W, Greengrass V, Plate M, Crowe SM, Sun Y.

Diagn Microbiol Infect Dis. 2010 Nov;68(3):208-13. doi: 10.1016/j.diagmicrobio.2010.06.007. Epub 2010 Sep 16.


The performance of reverse transcriptase assay for the estimation of the plasma viral load in HIV-1 and HIV-2 infections.

Padaki PA, Sachithanandham J, Isaac R, Ramalingam VV, Abraham OC, Pulimood SA, Kannangai R.

Infect Dis (Lond). 2016;48(6):467-71. doi: 10.3109/23744235.2015.1122832. Epub 2015 Dec 11.


Dried blood spot HIV-1 RNA quantification using open real-time systems in South Africa and Burkina Faso.

Viljoen J, Gampini S, Danaviah S, Valéa D, Pillay S, Kania D, Méda N, Newell ML, Van de Perre P, Rouet F; World Health Organization/ANRS 1289 Kesho Bora Study Group..

J Acquir Immune Defic Syndr. 2010 Nov;55(3):290-8. doi: 10.1097/QAI.0b013e3181edaaf5.


Dried blood spot HIV-1 RNA quantification: a useful tool for viral load monitoring among HIV-infected individuals in India.

Neogi U, Gupta S, Rodridges R, Sahoo PN, Rao SD, Rewari BB, Shastri S, Costa AD, Shet A.

Indian J Med Res. 2012 Dec;136(6):956-62.


Clinical comparison of branched DNA and reverse transcriptase-PCR and nucleic acid sequence-based amplification assay for the quantitation of circulating recombinant form_BC HIV-1 RNA in plasma.

Pan P, Tao X, Zhang Q, Xing W, Sun X, Pei L, Jiang Y.

AIDS. 2007 Dec;21 Suppl 8:S27-32. doi: 10.1097/01.aids.0000304693.16767.66.


Monitoring virologic responses to antiretroviral therapy in HIV-infected adults in Kenya: evaluation of a low-cost viral load assay.

Sivapalasingam S, Wangechi B, Marshed F, Laverty M, Essajee S, Holzman RS, Valentine F.

PLoS One. 2009 Aug 28;4(8):e6828. doi: 10.1371/journal.pone.0006828.


Transfer and evaluation of an automated, low-cost real-time reverse transcription-PCR test for diagnosis and monitoring of human immunodeficiency virus type 1 infection in a West African resource-limited setting.

Rouet F, Ekouevi DK, Chaix ML, Burgard M, Inwoley A, Tony TD, Danel C, Anglaret X, Leroy V, Msellati P, Dabis F, Rouzioux C.

J Clin Microbiol. 2005 Jun;43(6):2709-17.


A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting.

Acharya A, Vaniawala S, Shah P, Parekh H, Misra RN, Wani M, Mukhopadhyaya PN.

Biol Res. 2014 May 30;47:22. doi: 10.1186/0717-6287-47-22.


Evaluation of different RNA extraction methods and storage conditions of dried plasma or blood spots for human immunodeficiency virus type 1 RNA quantification and PCR amplification for drug resistance testing.

Monleau M, Montavon C, Laurent C, Segondy M, Montes B, Delaporte E, Boillot F, Peeters M.

J Clin Microbiol. 2009 Apr;47(4):1107-18. doi: 10.1128/JCM.02255-08. Epub 2009 Feb 4.


Plasma viral load concentrations in women and men from different exposure categories and with known duration of HIV infection. I.CO.N.A. Study Group.

Rezza G, Lepri AC, d'Arminio Monforte A, Pezzotti P, Castelli F, Dianzani F, Lazzarin A, De Luca A, Arlotti M, Leoncini F, Manconi PE, Rizzardini G, Minoli L, Poggio A, Ippolito G, Phillips AN, Moroni M.

J Acquir Immune Defic Syndr. 2000 Sep 1;25(1):56-62.


[Establishment of a real-time RT-PCR to detect plasma viral load of simian/human immunodeficiency virus CN97001 during its in vivo passage in rhesus monkeys].

Liu Q, Li J, Yang GB, Xing H, Dai JJ, Shao YM.

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2007 Jun;21(2):174-6. Chinese.


Validation and clinical use of a sensitive HIV-2 viral load assay that uses a whole virus internal control.

Styer LM, Miller TT, Parker MM.

J Clin Virol. 2013 Dec;58 Suppl 1:e127-33. doi: 10.1016/j.jcv.2013.09.007. Epub 2013 Sep 13.


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