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Items: 1 to 20 of 116

1.

abFASP-MS: affinity-based filter-aided sample preparation mass spectrometry for quantitative analysis of chemically labeled protein complexes.

Huber ML, Sacco R, Parapatics K, Skucha A, Khamina K, Müller AC, Rudashevskaya EL, Bennett KL.

J Proteome Res. 2014 Feb 7;13(2):1147-55. doi: 10.1021/pr4009892. Epub 2014 Jan 17.

2.

A method to resolve the composition of heterogeneous affinity-purified protein complexes assembled around a common protein by chemical cross-linking, gel electrophoresis and mass spectrometry.

Rudashevskaya EL, Sacco R, Kratochwill K, Huber ML, Gstaiger M, Superti-Furga G, Bennett KL.

Nat Protoc. 2013 Jan;8(1):75-97. doi: 10.1038/nprot.2012.133. Epub 2012 Dec 13.

PMID:
23237831
3.

Combining TBP-based rOFFGEL-IEF with FASP and nLC-ESI-LTQ-MS/MS for the analysis of cisplatin-binding proteins in rat kidney.

Moraleja I, Moreno-Gordaliza E, Mena ML, Gómez-Gómez MM.

Talanta. 2014 Mar;120:433-42. doi: 10.1016/j.talanta.2013.11.084. Epub 2013 Dec 22.

PMID:
24468393
4.

Identification of protein interacting partners using tandem affinity purification.

Bailey D, Urena L, Thorne L, Goodfellow I.

J Vis Exp. 2012 Feb 25;(60). pii: 3643. doi: 10.3791/3643.

5.
6.

Comparative reevaluation of FASP and enhanced FASP methods by LC-MS/MS.

Nel AJ, Garnett S, Blackburn JM, Soares NC.

J Proteome Res. 2015 Mar 6;14(3):1637-42. doi: 10.1021/pr501266c. Epub 2015 Feb 10.

PMID:
25619111
8.

A shotgun approach for the identification of platinum-protein complexes.

Moraleja I, Moreno-Gordaliza E, Esteban-Fernández D, Mena ML, Linscheid MW, Gómez-Gómez MM.

Anal Bioanal Chem. 2015 Mar;407(9):2393-403. doi: 10.1007/s00216-014-8452-x. Epub 2015 Jan 25.

PMID:
25618759
9.

An improved toolbox to unravel the plant cellular machinery by tandem affinity purification of Arabidopsis protein complexes.

Van Leene J, Eeckhout D, Cannoot B, De Winne N, Persiau G, Van De Slijke E, Vercruysse L, Dedecker M, Verkest A, Vandepoele K, Martens L, Witters E, Gevaert K, De Jaeger G.

Nat Protoc. 2015 Jan;10(1):169-87. doi: 10.1038/nprot.2014.199. Epub 2014 Dec 18.

PMID:
25521792
10.

Revealing novel telomere proteins using in vivo cross-linking, tandem affinity purification, and label-free quantitative LC-FTICR-MS.

Nittis T, Guittat L, LeDuc RD, Dao B, Duxin JP, Rohrs H, Townsend RR, Stewart SA.

Mol Cell Proteomics. 2010 Jun;9(6):1144-56. doi: 10.1074/mcp.M900490-MCP200. Epub 2010 Jan 22.

11.

High-throughput analysis of protein/peptide complexes by immunoprecipitation and automated LC-MS/MS.

Lin Z, Crockett DK, Lim MS, Elenitoba-Johnson KS.

J Biomol Tech. 2003 Jun;14(2):149-55.

13.

Shotgun proteomics: a relative quantitative approach using Off-Gel electrophoresis and LC-MS/MS.

Geiser L, Dayon L, Vaezzadeh AR, Hochstrasser DF.

Methods Mol Biol. 2011;681:459-72. doi: 10.1007/978-1-60761-913-0_27.

PMID:
20978983
14.

Combination of FASP and fully automated 2D-LC-MS/MS allows in-depth proteomic characterization of mouse zymogen granules.

Sun X, Jiang X.

Biomed Chromatogr. 2013 Mar;27(3):407-8. doi: 10.1002/bmc.2805. Epub 2012 Sep 4.

PMID:
22949036
15.

Systematic characterization of the protein interaction network and protein complexes in Saccharomyces cerevisiae using tandem affinity purification and mass spectrometry.

Babu M, Krogan NJ, Awrey DE, Emili A, Greenblatt JF.

Methods Mol Biol. 2009;548:187-207. doi: 10.1007/978-1-59745-540-4_11.

PMID:
19521826
16.

Comparison of detergent-based sample preparation workflows for LTQ-Orbitrap analysis of the Escherichia coli proteome.

Tanca A, Biosa G, Pagnozzi D, Addis MF, Uzzau S.

Proteomics. 2013 Sep;13(17):2597-607. doi: 10.1002/pmic.201200478. Epub 2013 Aug 7.

PMID:
23784971
18.

Interaction proteomics: characterization of protein complexes using tandem affinity purification-mass spectrometry.

Völkel P, Le Faou P, Angrand PO.

Biochem Soc Trans. 2010 Aug;38(4):883-7. doi: 10.1042/BST0380883. Review.

PMID:
20658971
19.

A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking.

Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.

Mol Cell Proteomics. 2006 Apr;5(4):737-48. Epub 2006 Jan 23.

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