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Items: 1 to 20 of 110

1.
2.

Simultaneous method for analyzing dimerization and signaling of G-protein-coupled receptor in yeast by dual-color reporter system.

Nakamura Y, Takemoto N, Ishii J, Kondo A.

Biotechnol Bioeng. 2014 Mar;111(3):586-96.

PMID:
24122388
3.

Quantitative and dynamic analyses of G protein-coupled receptor signaling in yeast using Fus1, enhanced green fluorescence protein (EGFP), and His3 fusion protein.

Ishii J, Matsumura S, Kimura S, Tatematsu K, Kuroda S, Fukuda H, Kondo A.

Biotechnol Prog. 2006 Jul-Aug;22(4):954-60.

PMID:
16889369
4.

Amplification of agonist stimulation of human G-protein-coupled receptor signaling in yeast.

Fukuda N, Ishii J, Kaishima M, Kondo A.

Anal Biochem. 2011 Oct 15;417(2):182-7. doi: 10.1016/j.ab.2011.06.006. Epub 2011 Jun 13.

PMID:
21726523
5.

Applications of yeast-based signaling sensor for characterization of antagonist and analysis of site-directed mutants of the human serotonin 1A receptor.

Nakamura Y, Ishii J, Kondo A.

Biotechnol Bioeng. 2015 Sep;112(9):1906-15. doi: 10.1002/bit.25597. Epub 2015 Jun 30.

PMID:
25850571
6.

Control of signalling properties of human somatostatin receptor subtype-5 by additional signal sequences on its amino-terminus in yeast.

Iguchi Y, Ishii J, Nakayama H, Ishikura A, Izawa K, Tanaka T, Ogino C, Kondo A.

J Biochem. 2010 Jun;147(6):875-84. doi: 10.1093/jb/mvq023. Epub 2010 Mar 5.

PMID:
20207822
7.

Microbial fluorescence sensing for human neurotensin receptor type 1 using Gα-engineered yeast cells.

Ishii J, Oda A, Togawa S, Fukao A, Fujiwara T, Ogino C, Kondo A.

Anal Biochem. 2014 Feb 1;446:37-43. doi: 10.1016/j.ab.2013.10.016. Epub 2013 Oct 17.

PMID:
24141076
8.

Yeast-based fluorescence reporter assay of G protein-coupled receptor signalling for flow cytometric screening: FAR1-disruption recovers loss of episomal plasmid caused by signalling in yeast.

Ishii J, Tanaka T, Matsumura S, Tatematsu K, Kuroda S, Ogino C, Fukuda H, Kondo A.

J Biochem. 2008 May;143(5):667-74. doi: 10.1093/jb/mvn018. Epub 2008 Feb 14.

PMID:
18281298
9.

Human G protein-coupled receptor studies in Saccharomyces cerevisiae.

Liu R, Wong W, IJzerman AP.

Biochem Pharmacol. 2016 Aug 15;114:103-15. doi: 10.1016/j.bcp.2016.02.010. Epub 2016 Feb 23. Review.

PMID:
26920251
10.

Neurotensin induces mating in Saccharomyces cerevisiae cells that express human neurotensin receptor type 1 in place of the endogenous pheromone receptor.

Leplatois P, Josse A, Guillemot M, Febvre M, Vita N, Ferrara P, Loison G.

Eur J Biochem. 2001 Sep;268(18):4860-7.

11.

A highly sensitive quantitative cytosensor technique for the identification of receptor ligands in tissue extracts.

Lenkei Z, Beaudet A, Chartrel N, De Mota N, Irinopoulou T, Braun B, Vaudry H, Llorens-Cortes C.

J Histochem Cytochem. 2000 Nov;48(11):1553-64.

PMID:
11036098
12.

Exceptional total and functional yields of the human adenosine (A2a) receptor expressed in the yeast Saccharomyces cerevisiae.

Niebauer RT, Robinson AS.

Protein Expr Purif. 2006 Apr;46(2):204-11. Epub 2005 Oct 24.

PMID:
16289981
13.

Construction of a yeast-based signaling biosensor for human angiotensin II type 1 receptor via functional coupling between Asn295-mutated receptor and Gpa1/Gi3 chimeric Gα.

Nakamura Y, Ishii J, Kondo A.

Biotechnol Bioeng. 2014 Nov;111(11):2220-8. doi: 10.1002/bit.25278. Epub 2014 Jul 18.

PMID:
24890663
14.

Cell wall trapping of autocrine peptides for human G-protein-coupled receptors on the yeast cell surface.

Ishii J, Yoshimoto N, Tatematsu K, Kuroda S, Ogino C, Fukuda H, Kondo A.

PLoS One. 2012;7(5):e37136. doi: 10.1371/journal.pone.0037136. Epub 2012 May 18.

15.

Positive Detection of GPCR Antagonists Using a System for Inverted Expression of a Fluorescent Reporter Gene.

Fukuda N, Kaishima M, Ishii J, Honda S.

ACS Synth Biol. 2017 May 18. doi: 10.1021/acssynbio.7b00056. [Epub ahead of print]

PMID:
28499341
16.

Transplantation of the GAL regulon into G-protein signaling circuitry in yeast.

Ryo S, Ishii J, Iguchi Y, Fukuda N, Kondo A.

Anal Biochem. 2012 May 1;424(1):27-31. doi: 10.1016/j.ab.2012.02.005. Epub 2012 Feb 13.

PMID:
22343189
17.
18.

A fluorescent alpha-factor analogue exhibits multiple steps on binding to its G protein coupled receptor in yeast.

Bajaj A, Celić A, Ding FX, Naider F, Becker JM, Dumont ME.

Biochemistry. 2004 Oct 26;43(42):13564-78.

PMID:
15491163
19.

Quantitative measurement of cell membrane receptor internalization by the nanoluciferase reporter: Using the G protein-coupled receptor RXFP3 as a model.

Liu Y, Song G, Shao XX, Liu YL, Guo ZY.

Biochim Biophys Acta. 2015 Feb;1848(2):688-94. doi: 10.1016/j.bbamem.2014.11.026. Epub 2014 Nov 28.

20.

Improved identification of agonist-mediated Gα(i)-specific human G-protein-coupled receptor signaling in yeast cells by flow cytometry.

Ishii J, Moriguchi M, Hara KY, Shibasaki S, Fukuda H, Kondo A.

Anal Biochem. 2012 Jul 15;426(2):129-33. doi: 10.1016/j.ab.2012.04.012. Epub 2012 Apr 11.

PMID:
22504120

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