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Items: 1 to 20 of 142

1.

Virus-free transient protein production in Sf9 cells.

Shen X, Hacker DL, Baldi L, Wurm FM.

J Biotechnol. 2014 Feb 10;171:61-70. doi: 10.1016/j.jbiotec.2013.11.018. Epub 2013 Dec 11.

PMID:
24333123
2.

A simple plasmid-based transient gene expression method using High Five cells.

Shen X, Pitol AK, Bachmann V, Hacker DL, Baldi L, Wurm FM.

J Biotechnol. 2015 Dec 20;216:67-75. doi: 10.1016/j.jbiotec.2015.10.007. Epub 2015 Oct 23.

PMID:
26476358
3.

Transient production of recombinant proteins by Chinese hamster ovary cells using polyethyleneimine/DNA complexes in combination with microtubule disrupting anti-mitotic agents.

Tait AS, Brown CJ, Galbraith DJ, Hines MJ, Hoare M, Birch JR, James DC.

Biotechnol Bioeng. 2004 Dec 20;88(6):707-21.

PMID:
15532040
4.

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells.

Hacker DL, Kiseljak D, Rajendra Y, Thurnheer S, Baldi L, Wurm FM.

Protein Expr Purif. 2013 Nov;92(1):67-76. doi: 10.1016/j.pep.2013.09.001. Epub 2013 Sep 8.

PMID:
24021764
5.

A simple high-yielding process for transient gene expression in CHO cells.

Rajendra Y, Kiseljak D, Baldi L, Hacker DL, Wurm FM.

J Biotechnol. 2011 Apr 20;153(1-2):22-6. doi: 10.1016/j.jbiotec.2011.03.001. Epub 2011 Mar 15.

PMID:
21392548
6.

Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells.

Galbraith DJ, Tait AS, Racher AJ, Birch JR, James DC.

Biotechnol Prog. 2006 May-Jun;22(3):753-62.

PMID:
16739959
7.

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems.

Muller N, Derouazi M, Van Tilborgh F, Wulhfard S, Hacker DL, Jordan M, Wurm FM.

Biotechnol Lett. 2007 May;29(5):703-11. Epub 2007 Feb 20.

PMID:
17310326
8.

A high-yielding CHO transient system: coexpression of genes encoding EBNA-1 and GS enhances transient protein expression.

Daramola O, Stevenson J, Dean G, Hatton D, Pettman G, Holmes W, Field R.

Biotechnol Prog. 2014 Jan-Feb;30(1):132-41. doi: 10.1002/btpr.1809. Epub 2013 Oct 10.

PMID:
24106171
9.

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection.

Chenuet S, Martinet D, Besuchet-Schmutz N, Wicht M, Jaccard N, Bon AC, Derouazi M, Hacker DL, Beckmann JS, Wurm FM.

Biotechnol Bioeng. 2008 Dec 1;101(5):937-45. doi: 10.1002/bit.21972.

PMID:
18781700
10.

Serum-free large-scale transient transfection of CHO cells.

Derouazi M, Girard P, Van Tilborgh F, Iglesias K, Muller N, Bertschinger M, Wurm FM.

Biotechnol Bioeng. 2004 Aug 20;87(4):537-45.

PMID:
15286991
11.

The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells.

Bertschinger M, Schertenleib A, Cevey J, Hacker DL, Wurm FM.

Mol Biotechnol. 2008 Oct;40(2):136-43. doi: 10.1007/s12033-008-9069-0. Epub 2008 Jun 10.

PMID:
18543131
12.

Identification of Essential Genetic Baculoviral Elements for Recombinant Protein Expression by Transactivation in Sf21 Insect Cells.

Bleckmann M, Schürig M, Chen FF, Yen ZZ, Lindemann N, Meyer S, Spehr J, van den Heuvel J.

PLoS One. 2016 Mar 2;11(3):e0149424. doi: 10.1371/journal.pone.0149424. eCollection 2016.

13.

A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: process development and product quality assessment.

Rajendra Y, Hougland MD, Alam R, Morehead TA, Barnard GC.

Biotechnol Bioeng. 2015 May;112(5):977-86. doi: 10.1002/bit.25514. Epub 2015 Mar 16.

PMID:
25502369
14.

High-level protein expression in scalable CHO transient transfection.

Ye J, Kober V, Tellers M, Naji Z, Salmon P, Markusen JF.

Biotechnol Bioeng. 2009 Jun 15;103(3):542-51. doi: 10.1002/bit.22265.

PMID:
19199356
15.

Characterization of a Trichoplusia ni hexamerin-derived promoter in the AcMNPV baculovirus vector.

López-Vidal J, Gómez-Sebastián S, Sánchez-Ramos I, Escribano JM.

J Biotechnol. 2013 Jun 10;165(3-4):201-8. doi: 10.1016/j.jbiotec.2013.03.012. Epub 2013 Apr 8.

PMID:
23578810
16.

High-level recombinant protein production in CHO cells using an adenoviral vector and the cumate gene-switch.

Gaillet B, Gilbert R, Amziani R, Guilbault C, Gadoury C, Caron AW, Mullick A, Garnier A, Massie B.

Biotechnol Prog. 2007 Jan-Feb;23(1):200-9.

PMID:
17269689
17.

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells.

Rajendra Y, Kiseljak D, Baldi L, Wurm FM, Hacker DL.

Biotechnol Prog. 2015 Mar-Apr;31(2):541-9. doi: 10.1002/btpr.2064. Epub 2015 Feb 26.

PMID:
25683738
18.
19.
20.

Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch.

Poulain A, Perret S, Malenfant F, Mullick A, Massie B, Durocher Y.

J Biotechnol. 2017 Aug 10;255:16-27. doi: 10.1016/j.jbiotec.2017.06.009. Epub 2017 Jun 15.

PMID:
28625678

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