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Items: 1 to 20 of 106

1.

Anti-idiotype scFv-enzyme fusion proteins: a clonable analyte-mimicking probe for standardized immunoassays targeting small biomarkers.

Oyama H, Tanaka E, Kawanaka T, Morita I, Niwa T, Kobayashi N.

Anal Chem. 2013 Dec 3;85(23):11553-9. doi: 10.1021/ac402868f. Epub 2013 Nov 20.

PMID:
24256209
2.
3.

[Noncompetitive immunoenzymometric assays for a hapten molecule employing anti-idiotype antibodies].

Niwa T, Kobayashi N, Goto J.

Rinsho Byori. 2008 Jun;56(6):536-43. Japanese.

PMID:
18646639
4.

Two-step in vitro antibody affinity maturation enables estradiol-17beta assays with more than 10-fold higher sensitivity.

Kobayashi N, Oyama H, Kato Y, Goto J, Söderlind E, Borrebaeck CA.

Anal Chem. 2010 Feb 1;82(3):1027-38. doi: 10.1021/ac902283n.

PMID:
20047279
5.
6.

"Breeding" diagnostic antibodies for higher assay performance: a 250-fold affinity-matured antibody mutant targeting a small biomarker.

Oyama H, Yamaguchi S, Nakata S, Niwa T, Kobayashi N.

Anal Chem. 2013 May 21;85(10):4930-7. doi: 10.1021/ac3037602. Epub 2013 Apr 30.

PMID:
23631394
8.

Generation of a single-chain Fv fragment for the monitoring of deoxycholic acid residues anchored on endogenous proteins.

Kobayashi N, Ohtoyo M, Wada E, Kato Y, Mano N, Goto J.

Steroids. 2005 Apr;70(4):285-94.

PMID:
15784283
9.

An alternative ELISA for T4 determination based on idiotype anti-idiotype interaction and a latex method for anti-idiotype monoclonal antibody selection.

Becker MI, Aguayo JE, Jamett A, Juica F, Yudelevich A, Foradori A, De Ioannes AE.

J Immunol Methods. 1996 Jun 10;192(1-2):73-85.

PMID:
8699024
10.

[Cloning of the variable region genes from hybridoma against HAAH and then construction and expression of anti-HAAH scFv].

Wang H, Xue XP, Lei YF, Song K, Hu YT, Wang W, Yang H.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 May;26(5):467-70. Chinese.

PMID:
20423655
11.

Single-chain Fv fragments derived from an anti-11-deoxycortisol antibody. Affinity, specificity, and idiotype analysis.

Kobayashi N, Shibahara K, Ikegashira K, Shibusawa K, Goto J.

Steroids. 2002 Jul;67(8):733-42.

PMID:
12117621
12.

Improved lectin ELISA for glycosylation analysis of biomarkers using PS-tag-fused single-chain Fv.

Kumada Y, Ohigashi Y, Emori Y, Imamura K, Omura Y, Kishimoto M.

J Immunol Methods. 2012 Nov 30;385(1-2):15-22. doi: 10.1016/j.jim.2012.07.021. Epub 2012 Aug 2.

PMID:
22884622
13.

Efficient refolding and immobilization of PMMA-tag-fused single-chain Fv antibodies for sensitive immunological detection on a PMMA plate.

Kumada Y, Ishikawa Y, Fujiwara Y, Takeda R, Miyamoto R, Niwa D, Momose S, Kang B, Kishimoto M.

J Immunol Methods. 2014 Sep;411:1-10. doi: 10.1016/j.jim.2014.05.015. Epub 2014 Jun 6.

PMID:
24910412
14.
15.

An improved phage-display panning method to produce an HM-1 killer toxin anti-idiotypic antibody.

Kabir ME, Krishnaswamy S, Miyamoto M, Furuichi Y, Komiyama T.

BMC Biotechnol. 2009 Dec 14;9:99. doi: 10.1186/1472-6750-9-99.

16.

Generation of anti-idiotype scFv for pharmacokinetic measurement in lymphoma patients treated with chimera anti-CD22 antibody SM03.

Zhao Q, Wong PF, Lee SS, Leung SO, Cheung WT, Wang JZ.

PLoS One. 2014 May 9;9(5):e96697. doi: 10.1371/journal.pone.0096697. eCollection 2014.

17.

Production and characterization of an anti-idiotypic single chain Fv that recognizes an anti-DNA antibody.

Kwon MH, Lee MS, Kim KH, Park S, Shin HJ, Jang YJ, Kim HI.

Immunol Invest. 2002 Aug-Nov;31(3-4):205-18.

PMID:
12472180
18.

Gaussia Luciferase as a Genetic Fusion Partner with Antibody Fragments for Sensitive Immunoassay Monitoring of Clinical Biomarkers.

Oyama H, Morita I, Kiguchi Y, Miyake S, Moriuchi A, Akisada T, Niwa T, Kobayashi N.

Anal Chem. 2015 Dec 15;87(24):12387-95. doi: 10.1021/acs.analchem.5b04015. Epub 2015 Dec 1.

19.

Production and characterization of a single-chain variable fragment linked alkaline phosphatase fusion protein for detection of O,O-diethyl organophosphorus pesticides in a one-step enzyme-linked immunosorbent assay.

Xu ZL, Dong JX, Wang H, Li ZF, Beier RC, Jiang YM, Lei HT, Shen YD, Yang JY, Sun YM.

J Agric Food Chem. 2012 May 23;60(20):5076-83. doi: 10.1021/jf300570q. Epub 2012 May 14.

PMID:
22533788
20.

An enzyme-linked immunometric assay for cortisol based on idiotype-anti-idiotype reactions.

Niwa T, Kobayashi T, Sun P, Goto J, Oyama H, Kobayashi N.

Anal Chim Acta. 2009 Apr 6;638(1):94-100. doi: 10.1016/j.aca.2009.02.010. Epub 2009 Feb 12.

PMID:
19298885

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