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Items: 1 to 20 of 94

1.

Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris.

Krainer FW, Gmeiner C, Neutsch L, Windwarder M, Pletzenauer R, Herwig C, Altmann F, Glieder A, Spadiut O.

Sci Rep. 2013 Nov 20;3:3279. doi: 10.1038/srep03279.

2.

[Pichia pastoris X-33 with OCH1 gene deletion and its expression of glycoprotein GM-CSF].

Zhang D, Xu Y, Xin X, Zhu R, Jin J.

Wei Sheng Wu Xue Bao. 2011 May;51(5):622-9. Chinese.

PMID:
21800624
3.

Protein Production with a Pichia pastoris OCH1 Knockout Strain in Fed-Batch Mode.

Gmeiner C, Spadiut O.

Methods Mol Biol. 2015;1321:91-101. doi: 10.1007/978-1-4939-2760-9_7.

PMID:
26082217
4.

Expression, purification and characterization of low-glycosylation influenza neuraminidase in α-1,6-mannosyltransferase defective Pichia pastoris.

Yang YL, Chang SH, Gong X, Wu J, Liu B.

Mol Biol Rep. 2012 Feb;39(2):857-64. doi: 10.1007/s11033-011-0809-z. Epub 2011 May 13.

PMID:
21567198
5.

Functional characterization of the Hansenula polymorpha HOC1, OCH1, and OCR1 genes as members of the yeast OCH1 mannosyltransferase family involved in protein glycosylation.

Kim MW, Kim EJ, Kim JY, Park JS, Oh DB, Shimma Y, Chiba Y, Jigami Y, Rhee SK, Kang HA.

J Biol Chem. 2006 Mar 10;281(10):6261-72. Epub 2006 Jan 10.

6.

Combining Protein and Strain Engineering for the Production of Glyco-Engineered Horseradish Peroxidase C1A in Pichia pastoris.

Capone S, Ćorajević L, Bonifert G, Murth P, Maresch D, Altmann F, Herwig C, Spadiut O.

Int J Mol Sci. 2015 Sep 24;16(10):23127-42. doi: 10.3390/ijms161023127.

7.

Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase.

Gmeiner C, Saadati A, Maresch D, Krasteva S, Frank M, Altmann F, Herwig C, Spadiut O.

Microb Cell Fact. 2015 Jan 8;14:1. doi: 10.1186/s12934-014-0183-3.

8.

[A Pichia pastoris with alpha-1, 6-mannosyltransferases deletion and its use in expression of HSA/GM-CSF chimera].

Wang Y, Gong X, Chang SH, Liu B, Song M, Huang HH, Wu J.

Sheng Wu Gong Cheng Xue Bao. 2007 Sep;23(5):907-14. Chinese.

PMID:
18051874
9.

Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man₈GlcNAc₂ and Man₅GlcNAc₂.

De Pourcq K, Vervecken W, Dewerte I, Valevska A, Van Hecke A, Callewaert N.

Microb Cell Fact. 2012 May 1;11:53. doi: 10.1186/1475-2859-11-53.

10.

Characterization of the Pichia pastoris protein-O-mannosyltransferase gene family.

Nett JH, Cook WJ, Chen MT, Davidson RC, Bobrowicz P, Kett W, Brevnova E, Potgieter TI, Mellon MT, Prinz B, Choi BK, Zha D, Burnina I, Bukowski JT, Du M, Wildt S, Hamilton SR.

PLoS One. 2013 Jul 1;8(7):e68325. doi: 10.1371/journal.pone.0068325. Print 2013.

11.

High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains--selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG.

Blanchard V, Gadkari RA, George AV, Roy S, Gerwig GJ, Leeflang BR, Dighe RR, Boelens R, Kamerling JP.

Glycoconj J. 2008 Apr;25(3):245-57. doi: 10.1007/s10719-007-9082-8. Epub 2008 Feb 15.

12.

Elimination of β-mannose glycan structures in Pichia pastoris.

Hopkins D, Gomathinayagam S, Rittenhour AM, Du M, Hoyt E, Karaveg K, Mitchell T, Nett JH, Sharkey NJ, Stadheim TA, Li H, Hamilton SR.

Glycobiology. 2011 Dec;21(12):1616-26. doi: 10.1093/glycob/cwr108. Epub 2011 Aug 12.

PMID:
21840970
13.

In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris.

Vervecken W, Kaigorodov V, Callewaert N, Geysens S, De Vusser K, Contreras R.

Appl Environ Microbiol. 2004 May;70(5):2639-46.

14.

Effects of different media supplements on the production of an active recombinant plant peroxidase in a Pichia pastoris Δoch1 strain.

Gmeiner C, Spadiut O.

Bioengineered. 2015;6(3):175-8. doi: 10.1080/21655979.2015.1036208. Epub 2015 Apr 2.

15.

Disruption of the OCH1 and MNN1 genes decrease N-glycosylation on glycoprotein expressed in Kluyveromyces lactis.

Liu B, Gong X, Chang S, Yang Y, Song M, Duan D, Wang L, Ma Q, Wu J.

J Biotechnol. 2009 Aug 20;143(2):95-102. doi: 10.1016/j.jbiotec.2009.06.016. Epub 2009 Jun 24.

PMID:
19559061
16.

Recombinant human lactoferrin expressed in glycoengineered Pichia pastoris: effect of terminal N-acetylneuraminic acid on in vitro secondary humoral immune response.

Choi BK, Actor JK, Rios S, d'Anjou M, Stadheim TA, Warburton S, Giaccone E, Cukan M, Li H, Kull A, Sharkey N, Gollnick P, Kocieba M, Artym J, Zimecki M, Kruzel ML, Wildt S.

Glycoconj J. 2008 Aug;25(6):581-93. doi: 10.1007/s10719-008-9123-y. Epub 2008 Mar 26.

17.

Production of a sterol esterase from Ophiostoma piceae in batch and fed-batch bioprocesses using different Pichia pastoris phenotypes as cell factory.

Cedillo VB, Martínez MJ, Arnau C, Valero F.

Biotechnol Prog. 2014 Sep-Oct;30(5):1012-20. doi: 10.1002/btpr.1939. Epub 2014 Jun 26.

PMID:
24930588
18.

Engineering of an artificial glycosylation pathway blocked in core oligosaccharide assembly in the yeast Pichia pastoris: production of complex humanized glycoproteins with terminal galactose.

Bobrowicz P, Davidson RC, Li H, Potgieter TI, Nett JH, Hamilton SR, Stadheim TA, Miele RG, Bobrowicz B, Mitchell T, Rausch S, Renfer E, Wildt S.

Glycobiology. 2004 Sep;14(9):757-66. Epub 2004 Jun 9.

PMID:
15190003
19.
20.

Identification of a new family of genes involved in beta-1,2-mannosylation of glycans in Pichia pastoris and Candida albicans.

Mille C, Bobrowicz P, Trinel PA, Li H, Maes E, Guerardel Y, Fradin C, Martínez-Esparza M, Davidson RC, Janbon G, Poulain D, Wildt S.

J Biol Chem. 2008 Apr 11;283(15):9724-36. doi: 10.1074/jbc.M708825200. Epub 2008 Jan 30.

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