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Items: 1 to 20 of 92

1.

Establishing a versatile Golden Gate cloning system for genetic engineering in fungi.

Terfrüchte M, Joehnk B, Fajardo-Somera R, Braus GH, Riquelme M, Schipper K, Feldbrügge M.

Fungal Genet Biol. 2014 Jan;62:1-10. doi: 10.1016/j.fgb.2013.10.012. Epub 2013 Nov 8.

PMID:
24211735
2.

DelsGate, a robust and rapid gene deletion construction method.

García-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold SE.

Fungal Genet Biol. 2008 Apr;45(4):379-88. doi: 10.1016/j.fgb.2007.11.001. Epub 2007 Nov 19.

PMID:
18248826
3.

A PCR-based system for highly efficient generation of gene replacement mutants in Ustilago maydis.

Kämper J.

Mol Genet Genomics. 2004 Feb;271(1):103-10. Epub 2003 Dec 12.

PMID:
14673645
4.

DelsGate: a robust and rapid method for gene deletion.

García-Pedrajas MD, Nadal M, Denny T, Baeza-Montañez L, Paz Z, Gold SE.

Methods Mol Biol. 2010;638:55-76. doi: 10.1007/978-1-60761-611-5_5.

PMID:
20238261
5.

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions.

Bösch K, Frantzeskakis L, Vraneš M, Kämper J, Schipper K, Göhre V.

J Vis Exp. 2016 Sep 30;(115). doi: 10.3791/54522.

PMID:
27768088
6.

A reverse genetic approach for generating gene replacement mutants in Ustilago maydis.

Brachmann A, König J, Julius C, Feldbrügge M.

Mol Genet Genomics. 2004 Sep;272(2):216-26. Epub 2004 Aug 17. Erratum in: Mol Genet Genomics. 2004 Nov;272(4):488.

PMID:
15316769
7.

Generation of families of construct variants using golden gate shuffling.

Engler C, Marillonnet S.

Methods Mol Biol. 2011;729:167-81. doi: 10.1007/978-1-61779-065-2_11.

PMID:
21365490
8.

Regulation of Ustilago maydis dimorphism, sporulation, and pathogenic development by a transcription factor with a highly conserved APSES domain.

García-Pedrajas MD, Baeza-Montañez L, Gold SE.

Mol Plant Microbe Interact. 2010 Feb;23(2):211-22. doi: 10.1094/MPMI-23-2-0211.

9.

Cloning and disruption of Ustilago maydis genes.

Fotheringham S, Holloman WK.

Mol Cell Biol. 1989 Sep;9(9):4052-5.

10.

GreenGate---a novel, versatile, and efficient cloning system for plant transgenesis.

Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J.

PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. eCollection 2013.

11.

Transient disruption of non-homologous end-joining facilitates targeted genome manipulations in the filamentous fungus Aspergillus nidulans.

Nielsen JB, Nielsen ML, Mortensen UH.

Fungal Genet Biol. 2008 Mar;45(3):165-70. Epub 2007 Jul 20.

PMID:
17703973
12.

Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs.

Kirchmaier S, Lust K, Wittbrodt J.

PLoS One. 2013 Oct 7;8(10):e76117. doi: 10.1371/journal.pone.0076117. eCollection 2013.

13.

The isolation of a Dol-P-Man synthase from Ustilago maydis that functions in Saccharomyces cerevisiae.

Zimmerman JW, Specht CA, Cazares BX, Robbins PW.

Yeast. 1996 Jun 30;12(8):765-71.

14.

Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue.

Krappmann S, Braus GH.

Mol Genet Genomics. 2003 Feb;268(5):675-83. Epub 2003 Jan 15.

PMID:
12589442
15.

Conversion of BAC clones into binary BAC (BIBAC) vectors and their delivery into basidiomycete fungal cells using Agrobacterium tumefaciens.

Ali S, Bakkeren G.

Methods Mol Biol. 2015;1227:199-215. doi: 10.1007/978-1-4939-1652-8_9.

PMID:
25239747
17.

A golden gate modular cloning toolbox for plants.

Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, Patron NJ, Marillonnet S.

ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. Epub 2014 Feb 20.

PMID:
24933124
18.

Gene expression during Ustilago maydis diploid filamentous growth: EST library creation and analyses.

Nugent KG, Choffe K, Saville BJ.

Fungal Genet Biol. 2004 Mar;41(3):349-60.

PMID:
14761795
19.
20.

Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi.

Frandsen RJ, Andersson JA, Kristensen MB, Giese H.

BMC Mol Biol. 2008 Aug 1;9:70. doi: 10.1186/1471-2199-9-70.

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