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Items: 1 to 20 of 125

1.

Single-nucleotide polymorphisms and other mismatches reduce performance of quantitative PCR assays.

Lefever S, Pattyn F, Hellemans J, Vandesompele J.

Clin Chem. 2013 Oct;59(10):1470-80. doi: 10.1373/clinchem.2013.203653.

2.

The effect of primer-template mismatches on the detection and quantification of nucleic acids using the 5' nuclease assay.

Stadhouders R, Pas SD, Anber J, Voermans J, Mes TH, Schutten M.

J Mol Diagn. 2010 Jan;12(1):109-17. doi: 10.2353/jmoldx.2010.090035.

3.

Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR.

Boyle B, Dallaire N, MacKay J.

BMC Biotechnol. 2009 Aug 28;9:75. doi: 10.1186/1472-6750-9-75.

4.
5.

Assessment of primer/template mismatch effects on real-time PCR amplification of target taxa for GMO quantification.

Ghedira R, Papazova N, Vuylsteke M, Ruttink T, Taverniers I, De Loose M.

J Agric Food Chem. 2009 Oct 28;57(20):9370-7. doi: 10.1021/jf901976a.

PMID:
19778057
6.

Deconstructing the polymerase chain reaction: understanding and correcting bias associated with primer degeneracies and primer-template mismatches.

Green SJ, Venkatramanan R, Naqib A.

PLoS One. 2015 May 21;10(5):e0128122. doi: 10.1371/journal.pone.0128122.

7.

Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods.

Piñol J, Mir G, Gomez-Polo P, Agustí N.

Mol Ecol Resour. 2015 Jul;15(4):819-30. doi: 10.1111/1755-0998.12355.

PMID:
25454249
8.

RExPrimer: an integrated primer designing tool increases PCR effectiveness by avoiding 3' SNP-in-primer and mis-priming from structural variation.

Piriyapongsa J, Ngamphiw C, Assawamakin A, Wangkumhang P, Suwannasri P, Ruangrit U, Agavatpanitch G, Tongsima S.

BMC Genomics. 2009 Dec 3;10 Suppl 3:S4. doi: 10.1186/1471-2164-10-S3-S4.

10.

Differential haplotype amplification leads to misgenotyping of heterozygote as homozygote when using single nucleotide mismatch primer.

De Sarkar N, Majumder M, Roy B.

Electrophoresis. 2012 Dec;33(23):3564-73. doi: 10.1002/elps.201200363.

PMID:
23161286
12.
13.

Studying the effect of single mismatches in primer and probe binding regions on amplification curves and quantification in real-time PCR.

Süss B, Flekna G, Wagner M, Hein I.

J Microbiol Methods. 2009 Mar;76(3):316-9. doi: 10.1016/j.mimet.2008.12.003.

PMID:
19135484
14.

Quantitative effects of position and type of single mismatch on single base primer extension.

Wu JH, Hong PY, Liu WT.

J Microbiol Methods. 2009 Jun;77(3):267-75. doi: 10.1016/j.mimet.2009.03.001.

PMID:
19285527
15.

Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies.

Kwok S, Kellogg DE, McKinney N, Spasic D, Goda L, Levenson C, Sninsky JJ.

Nucleic Acids Res. 1990 Feb 25;18(4):999-1005.

16.

Discrimination of primer 3'-nucleotide mismatch by taq DNA polymerase during polymerase chain reaction.

Ayyadevara S, Thaden JJ, Shmookler Reis RJ.

Anal Biochem. 2000 Aug 15;284(1):11-8.

PMID:
10933850
17.

Design of allele-specific primers and detection of the human ABO genotyping to avoid the pseudopositive problem.

Yaku H, Yukimasa T, Nakano S, Sugimoto N, Oka H.

Electrophoresis. 2008 Nov;29(20):4130-40. doi: 10.1002/elps.200800097.

PMID:
18991264
18.

Robustness of single-base extension against mismatches at the site of primer attachment in a clinical assay.

Kirsten H, Teupser D, Weissfuss J, Wolfram G, Emmrich F, Ahnert P.

J Mol Med (Berl). 2007 Apr;85(4):361-9.

PMID:
17160404
19.

PIRA PCR designer for restriction analysis of single nucleotide polymorphisms.

Ke X, Collins A, Ye S.

Bioinformatics. 2001 Sep;17(9):838-9.

PMID:
11590100
20.

Sequence variation in primer targets affects the accuracy of viral quantitative PCR.

Whiley DM, Sloots TP.

J Clin Virol. 2005 Oct;34(2):104-7.

PMID:
16157260
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