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Items: 1 to 20 of 95

1.

High level expression, efficient purification and bioactivity assay of recombinant human platelet-derived growth factor AA dimer (PDGF-AA) from methylotrophic yeast Pichia pastoris.

Li H, Hui X, Yang S, Hu X, Tang X, Li P, Li S, Yang L, Jin S, Wang Y, Xu A, Wu D.

Protein Expr Purif. 2013 Oct;91(2):221-7. doi: 10.1016/j.pep.2013.08.008. Epub 2013 Aug 23.

PMID:
23978536
2.

Identification and Functional Characterization of Glycosylation of Recombinant Human Platelet-Derived Growth Factor-BB in Pichia pastoris.

Dai M, Yu C, Fang T, Fu L, Wang J, Zhang J, Ren J, Xu J, Zhang X, Chen W.

PLoS One. 2015 Dec 23;10(12):e0145419. doi: 10.1371/journal.pone.0145419. eCollection 2015.

3.

Expression, purification and biological characterization of human vasostatin120-180 in Pichia pastoris.

Lin Y, Yang X, Lu M, Zhuang H, Hua ZC.

Protein Expr Purif. 2013 Dec;92(2):141-7. doi: 10.1016/j.pep.2013.09.016. Epub 2013 Oct 1.

PMID:
24096091
4.

High level expression, efficient purification, and bioactivity of recombinant human metallothionein 3 (rhMT3) from methylotrophic yeast Pichia pastoris.

Wu Q, Li B, Wu F, Yang L, Li S, Li H, Wu D, Cui T, Tang D.

Protein Expr Purif. 2014 Sep;101:121-6. doi: 10.1016/j.pep.2014.06.009. Epub 2014 Jun 25.

PMID:
24973778
5.

A cationic region of the platelet-derived growth factor (PDGF) A-chain (Arg159-Lys160-Lys161) is required for receptor binding and mitogenic activity of the PDGF-AA homodimer.

Fenstermaker RA, Poptic E, Bonfield TL, Knauss TC, Corsillo L, Piskurich JF, Kaetzel CS, Jentoft JE, Gelfand C, DiCorleto PE, et al.

J Biol Chem. 1993 May 15;268(14):10482-9.

6.

High-level expression, purification, and characterization of recombinant human basic fibroblast growth factor in Pichia pastoris.

Mu X, Kong N, Chen W, Zhang T, Shen M, Yan W.

Protein Expr Purif. 2008 Jun;59(2):282-8. doi: 10.1016/j.pep.2008.02.009. Epub 2008 Feb 29.

PMID:
18378165
7.

Expression of the GM2-activator protein in the methylotrophic yeast Pichia pastoris, purification, isotopic labeling, and biophysical characterization.

Wendeler M, Hoernschemeyer J, John M, Werth N, Schoeniger M, Lemm T, Hartmann R, Kessler H, Sandhoff K.

Protein Expr Purif. 2004 Mar;34(1):147-57.

PMID:
14766311
8.

High level expression and purification of active recombinant human interleukin-8 in Pichia pastoris.

Li H, Wang D, Xu A, Li S, Jin S, Wu D.

Protein Expr Purif. 2009 Nov;68(1):60-4. doi: 10.1016/j.pep.2009.06.010. Epub 2009 Jun 21.

PMID:
19545634
9.

Expression, purification and characterization of low-glycosylation influenza neuraminidase in α-1,6-mannosyltransferase defective Pichia pastoris.

Yang YL, Chang SH, Gong X, Wu J, Liu B.

Mol Biol Rep. 2012 Feb;39(2):857-64. doi: 10.1007/s11033-011-0809-z. Epub 2011 May 13.

PMID:
21567198
10.

High level expression of active recombinant human interleukin-3 in Pichia pastoris.

Li H, Li N, Gao X, Kong X, Li S, Xu A, Jin S, Wu D.

Protein Expr Purif. 2011 Dec;80(2):185-93. doi: 10.1016/j.pep.2011.08.027. Epub 2011 Aug 31.

PMID:
21907288
11.

Expression, purification and characterization of recombinant human interleukin-22 in Pichia pastoris.

Cai X, Wang J, Wang Y, Yang Y, Gao J, Fu W, Wang J, Xu D.

Mol Biol Rep. 2010 Jul;37(6):2609-13. doi: 10.1007/s11033-009-9785-y. Epub 2009 Sep 16.

PMID:
19760099
12.

Incomplete process of recombinant human platelet-derived growth factor produced in yeast and its effect on the biological activity.

Calderón-Cacia M, Tekamp-Olson P, Allen J, George-Nascimento C.

Biochem Biophys Res Commun. 1992 Sep 16;187(2):1193-9.

PMID:
1530614
13.

Differential binding, biological and biochemical actions of recombinant PDGF AA, AB, and BB molecules on connective tissue cells.

Grotendorst GR, Igarashi A, Larson R, Soma Y, Charette M.

J Cell Physiol. 1991 Nov;149(2):235-43.

PMID:
1660900
14.

Large-scale production, purification, and characterisation of recombinant Phaseolus vulgaris phytohemagglutinin E-form expressed in the methylotrophic yeast Pichia pastoris.

Baumgartner P, Raemaekers RJ, Durieux A, Gatehouse A, Davies H, Taylor M.

Protein Expr Purif. 2002 Dec;26(3):394-405.

PMID:
12460763
15.
16.

Expression and purification of glycosylated bovine beta-casein (L70S/P71S) in Pichia pastoris.

Choi BK, Jiménez-Flores R.

J Agric Food Chem. 2001 Apr;49(4):1761-6.

PMID:
11308323
17.

High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains--selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG.

Blanchard V, Gadkari RA, George AV, Roy S, Gerwig GJ, Leeflang BR, Dighe RR, Boelens R, Kamerling JP.

Glycoconj J. 2008 Apr;25(3):245-57. doi: 10.1007/s10719-007-9082-8. Epub 2008 Feb 15.

18.

High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris.

Sreekrishna K, Nelles L, Potenz R, Cruze J, Mazzaferro P, Fish W, Fuke M, Holden K, Phelps D, Wood P, et al.

Biochemistry. 1989 May 2;28(9):4117-25.

PMID:
2752013
19.

Differences in substrate specificities of alpha and beta platelet-derived growth factor (PDGF) receptors. Correlation with their ability to mediate PDGF transforming functions.

Heidaran MA, Beeler JF, Yu JC, Ishibashi T, LaRochelle WJ, Pierce JH, Aaronson SA.

J Biol Chem. 1993 May 5;268(13):9287-95.

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