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Items: 1 to 20 of 143

1.

Efficient agroinfiltration of plants for high-level transient expression of recombinant proteins.

Leuzinger K, Dent M, Hurtado J, Stahnke J, Lai H, Zhou X, Chen Q.

J Vis Exp. 2013 Jul 23;(77). doi: 10.3791/50521.

2.

Agroinfiltration as an Effective and Scalable Strategy of Gene Delivery for Production of Pharmaceutical Proteins.

Chen Q, Lai H, Hurtado J, Stahnke J, Leuzinger K, Dent M.

Adv Tech Biol Med. 2013 Jun;1(1). pii: 103.

3.

Transient Protein Expression by Agroinfiltration in Lettuce.

Chen Q, Dent M, Hurtado J, Stahnke J, McNulty A, Leuzinger K, Lai H.

Methods Mol Biol. 2016;1385:55-67. doi: 10.1007/978-1-4939-3289-4_4.

PMID:
26614281
4.

Expression of synthetic human tropoelastin (hTE) protein in Nicotiana tabacum.

Abdelghani M, El-Heba GA, Abdelhadi AA, Abdallah NA.

GM Crops Food. 2015;6(1):54-62. doi: 10.1080/21645698.2015.1026524.

5.
6.

Animal component-free Agrobacterium tumefaciens cultivation media for better GMP-compliance increases biomass yield and pharmaceutical protein expression in Nicotiana benthamiana.

Houdelet M, Galinski A, Holland T, Wenzel K, Schillberg S, Buyel JF.

Biotechnol J. 2017 Apr;12(4). doi: 10.1002/biot.201600721. Epub 2017 Mar 16.

PMID:
28221723
7.

Agrobacterium tumefaciens-mediated transient transformation of Arabidopsis thaliana leaves.

Mangano S, Gonzalez CD, Petruccelli S.

Methods Mol Biol. 2014;1062:165-73. doi: 10.1007/978-1-62703-580-4_8.

PMID:
24057365
8.

Tackling heterogeneity: a leaf disc-based assay for the high-throughput screening of transient gene expression in tobacco.

Piotrzkowski N, Schillberg S, Rasche S.

PLoS One. 2012;7(9):e45803. doi: 10.1371/journal.pone.0045803. Epub 2012 Sep 21.

9.

A simple agroinfiltration method for transient gene expression in plant leaf discs.

Matsuo K, Fukuzawa N, Matsumura T.

J Biosci Bioeng. 2016 Sep;122(3):351-6. doi: 10.1016/j.jbiosc.2016.02.001. Epub 2016 Mar 16.

PMID:
26995064
10.

Optimization of the bioprocessing conditions for scale-up of transient production of a heterologous protein in plants using a chemically inducible viral amplicon expression system.

Plesha MA, Huang TK, Dandekar AM, Falk BW, McDonald KA.

Biotechnol Prog. 2009 May-Jun;25(3):722-34. doi: 10.1002/btpr.149.

PMID:
19504593
11.

[A hydroponic cultivation system for rapid high-yield transient protein expression in Nicotiana plants under laboratory conditions].

Mo Q, Mai R, Yang Z, Chen M, Yang T, Lai H, Yang P, Chen Q, Zhou X.

Nan Fang Yi Ke Da Xue Xue Bao. 2012 Jun;32(6):772-7. Chinese.

12.

Efficient Agrobacterium-based transient expression system for the production of biopharmaceuticals in plants.

Circelli P, Donini M, Villani ME, Benvenuto E, Marusic C.

Bioeng Bugs. 2010 May-Jun;1(3):221-4. doi: 10.4161/bbug.1.3.11722. Epub 2010 Mar 2.

13.

Growth kinetics and scale-up of Agrobacterium tumefaciens.

Leth IK, McDonald KA.

Appl Microbiol Biotechnol. 2017 Jun;101(12):4895-4903. doi: 10.1007/s00253-017-8241-5. Epub 2017 Mar 29.

PMID:
28357545
14.

The development, characterization, and demonstration of a novel strategy for purification of recombinant proteins expressed in plants.

Tremblay R, Diao H, Huner N, Jevnikar AM, Ma S.

Transgenic Res. 2011 Dec;20(6):1357-66. doi: 10.1007/s11248-011-9498-6. Epub 2011 Mar 2.

PMID:
21365323
15.

A novel and fully scalable Agrobacterium spray-based process for manufacturing cellulases and other cost-sensitive proteins in plants.

Hahn S, Giritch A, Bartels D, Bortesi L, Gleba Y.

Plant Biotechnol J. 2015 Jun;13(5):708-16. doi: 10.1111/pbi.12299. Epub 2014 Dec 2.

16.

The impact of Pseudomonas syringae type III effectors on transient protein expression in tobacco.

Buyel JF, Buyel JJ, Haase C, Fischer R.

Plant Biol (Stuttg). 2015 Mar;17(2):484-92. doi: 10.1111/plb.12264. Epub 2014 Nov 25.

PMID:
25243954
17.

Coordinate expression of multiple proteins in plant cells by exploiting endogenous kex2p-like protease activity.

Zhang B, Rapolu M, Huang L, Su WW.

Plant Biotechnol J. 2011 Dec;9(9):970-81. doi: 10.1111/j.1467-7652.2011.00607.x. Epub 2011 Mar 28.

18.

Optimization and utilization of Agrobacterium-mediated transient protein production in Nicotiana.

Shamloul M, Trusa J, Mett V, Yusibov V.

J Vis Exp. 2014 Apr 19;(86). doi: 10.3791/51204.

19.

The pEAQ vector series: the easy and quick way to produce recombinant proteins in plants.

Peyret H, Lomonossoff GP.

Plant Mol Biol. 2013 Sep;83(1-2):51-8. doi: 10.1007/s11103-013-0036-1. Epub 2013 Mar 12. Review.

PMID:
23479085
20.

Inducible Expression of Agrobacterium Virulence Gene VirE2 for Stringent Regulation of T-DNA Transfer in Plant Transient Expression Systems.

Denkovskienė E, Paškevičius Š, Werner S, Gleba Y, Ražanskienė A.

Mol Plant Microbe Interact. 2015 Nov;28(11):1247-55. doi: 10.1094/MPMI-05-15-0102-R. Epub 2015 Oct 12.

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