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Items: 1 to 20 of 86


Hierarchical modeling for rare event detection and cell subset alignment across flow cytometry samples.

Cron A, Gouttefangeas C, Frelinger J, Lin L, Singh SK, Britten CM, Welters MJ, van der Burg SH, West M, Chan C.

PLoS Comput Biol. 2013;9(7):e1003130. doi: 10.1371/journal.pcbi.1003130. Epub 2013 Jul 11.


The development of standard samples with a defined number of antigen-specific T cells to harmonize T cell assays: a proof-of-principle study.

Singh SK, Tummers B, Schumacher TN, Gomez R, Franken KL, Verdegaal EM, Laske K, Gouttefangeas C, Ottensmeier C, Welters MJ, Britten CM, van der Burg SH.

Cancer Immunol Immunother. 2013 Mar;62(3):489-501. doi: 10.1007/s00262-012-1351-0. Epub 2012 Sep 18.


Data analysis as a source of variability of the HLA-peptide multimer assay: from manual gating to automated recognition of cell clusters.

Gouttefangeas C, Chan C, Attig S, Køllgaard TT, Rammensee HG, Stevanović S, Wernet D, thor Straten P, Welters MJ, Ottensmeier C, van der Burg SH, Britten CM.

Cancer Immunol Immunother. 2015 May;64(5):585-98. doi: 10.1007/s00262-014-1649-1. Epub 2015 Feb 18.


Statistical mixture modeling for cell subtype identification in flow cytometry.

Chan C, Feng F, Ottinger J, Foster D, West M, Kepler TB.

Cytometry A. 2008 Aug;73(8):693-701. doi: 10.1002/cyto.a.20583.


The CIMT-monitoring panel: a two-step approach to harmonize the enumeration of antigen-specific CD8+ T lymphocytes by structural and functional assays.

Britten CM, Gouttefangeas C, Welters MJ, Pawelec G, Koch S, Ottensmeier C, Mander A, Walter S, Paschen A, Müller-Berghaus J, Haas I, Mackensen A, Køllgaard T, thor Straten P, Schmitt M, Giannopoulos K, Maier R, Veelken H, Bertinetti C, Konur A, Huber C, Stevanović S, Wölfel T, van der Burg SH.

Cancer Immunol Immunother. 2008 Mar;57(3):289-302. Epub 2007 Aug 25.


Identification and visualization of multidimensional antigen-specific T-cell populations in polychromatic cytometry data.

Lin L, Frelinger J, Jiang W, Finak G, Seshadri C, Bart PA, Pantaleo G, McElrath J, DeRosa S, Gottardo R.

Cytometry A. 2015 Jul;87(7):675-82. doi: 10.1002/cyto.a.22623. Epub 2015 Apr 23.


Setting objective thresholds for rare event detection in flow cytometry.

Richards AJ, Staats J, Enzor J, McKinnon K, Frelinger J, Denny TN, Weinhold KJ, Chan C.

J Immunol Methods. 2014 Jul;409:54-61. doi: 10.1016/j.jim.2014.04.002. Epub 2014 Apr 12.


An Italian national multicenter study for the definition of reference ranges for normal values of peripheral blood lymphocyte subsets in healthy adults.

Santagostino A, Garbaccio G, Pistorio A, Bolis V, Camisasca G, Pagliaro P, Girotto M.

Haematologica. 1999 Jun;84(6):499-504.


Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients.

Xu Y, Theobald V, Sung C, DePalma K, Atwater L, Seiger K, Perricone MA, Richards SM.

J Transl Med. 2008 Oct 22;6:61. doi: 10.1186/1479-5876-6-61.


SWIFT-scalable clustering for automated identification of rare cell populations in large, high-dimensional flow cytometry datasets, part 2: biological evaluation.

Mosmann TR, Naim I, Rebhahn J, Datta S, Cavenaugh JS, Weaver JM, Sharma G.

Cytometry A. 2014 May;85(5):422-33. doi: 10.1002/cyto.a.22445. Epub 2014 Feb 14.


Hierarchical Bayesian mixture modelling for antigen-specific T-cell subtyping in combinatorially encoded flow cytometry studies.

Lin L, Chan C, Hadrup SR, Froesig TM, Wang Q, West M.

Stat Appl Genet Mol Biol. 2013 Jun;12(3):309-31. doi: 10.1515/sagmb-2012-0001.


Automated Analysis of Flow Cytometry Data to Reduce Inter-Lab Variation in the Detection of Major Histocompatibility Complex Multimer-Binding T Cells.

Pedersen NW, Chandran PA, Qian Y, Rebhahn J, Petersen NV, Hoff MD, White S, Lee AJ, Stanton R, Halgreen C, Jakobsen K, Mosmann T, Gouttefangeas C, Chan C, Scheuermann RH, Hadrup SR.

Front Immunol. 2017 Jul 26;8:858. doi: 10.3389/fimmu.2017.00858. eCollection 2017.


Simultaneous flow cytometric analysis of two cell surface markers, telomere length, and DNA content.

Schmid I, Dagarag MD, Hausner MA, Matud JL, Just T, Effros RB, Jamieson BD.

Cytometry. 2002 Nov 1;49(3):96-105.


[A flow cytometric assay for the expression of interferon gamma in T lymphocytes and its application in the study of EIAV-induced immune response].

Lin Y, Deng X, Shen N, Zhao L, Meng Q, Max J, Wang J, Shao Y, Zhou J.

Wei Sheng Wu Xue Bao. 2008 Jun;48(6):800-5. Chinese.


Dextramer reagents are effective tools for quantifying CMV antigen-specific T cells from peripheral blood samples.

Tario JD Jr, Chen GL, Hahn TE, Pan D, Furlage RL, Zhang Y, Brix L, Halgreen C, Jacobsen K, McCarthy PL, Wallace PK.

Cytometry B Clin Cytom. 2015 Jan;88(1):6-20. doi: 10.1002/cyto.b.21196. Epub 2014 Oct 23.


The simultaneous ex vivo detection of low-frequency antigen-specific CD4+ and CD8+ T-cell responses using overlapping peptide pools.

Singh SK, Meyering M, Ramwadhdoebe TH, Stynenbosch LF, Redeker A, Kuppen PJ, Melief CJ, Welters MJ, van der Burg SH.

Cancer Immunol Immunother. 2012 Nov;61(11):1953-63. doi: 10.1007/s00262-012-1251-3. Epub 2012 Apr 11.


Flow cytometry for natural killer T cells: multi-parameter methods for multifunctional cells.

Metelitsa LS.

Clin Immunol. 2004 Mar;110(3):267-76. Review.


Enumeration of antigen-specific CD8+ T lymphocytes by single-platform, HLA tetramer-based flow cytometry: a European multicenter evaluation.

Heijnen IA, Barnett D, Arroz MJ, Barry SM, Bonneville M, Brando B, D'hautcourt JL, Kern F, Tötterman TH, Marijt EW, Bossy D, Preijers FW, Rothe G, Gratama JW; European Working Group on Clinical Cell Analysis.

Cytometry B Clin Cytom. 2004 Nov;62(1):1-13.


Exploring the feasibility of multi-site flow cytometric processing of gut associated lymphoid tissue with centralized data analysis for multi-site clinical trials.

McGowan I, Anton PA, Elliott J, Cranston RD, Duffill K, Althouse AD, Hawkins KL, De Rosa SC.

PLoS One. 2015 May 26;10(5):e0126454. doi: 10.1371/journal.pone.0126454. eCollection 2015.


A new automated flow cytometry data analysis approach for the diagnostic screening of neoplastic B-cell disorders in peripheral blood samples with absolute lymphocytosis.

Costa ES, Arroyo ME, Pedreira CE, García-Marcos MA, Tabernero MD, Almeida J, Orfao A.

Leukemia. 2006 Jul;20(7):1221-30. Epub 2006 May 25.


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