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Items: 1 to 20 of 98

1.

Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure.

Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T.

FEBS Lett. 2013 Aug 19;587(16):2691-7. doi: 10.1016/j.febslet.2013.07.008. Epub 2013 Jul 18.

2.

Crystal structure and chitin oligosaccharide-binding mode of a 'loopful' family GH19 chitinase from rye, Secale cereale, seeds.

Ohnuma T, Numata T, Osawa T, Inanaga H, Okazaki Y, Shinya S, Kondo K, Fukuda T, Fukamizo T.

FEBS J. 2012 Oct;279(19):3639-3651. doi: 10.1111/j.1742-4658.2012.08723.x. Epub 2012 Sep 3.

3.

Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center.

Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T.

Biochim Biophys Acta. 2014 Apr;1844(4):793-802. doi: 10.1016/j.bbapap.2014.02.013. Epub 2014 Feb 25.

PMID:
24582745
4.

Enzymatic properties of a GH19 chitinase isolated from rice lacking a major loop structure involved in chitin binding.

Tanaka J, Fukamizo T, Ohnuma T.

Glycobiology. 2017 May 1;27(5):477-485. doi: 10.1093/glycob/cwx016.

PMID:
28204489
5.

A glycosynthase derived from an inverting chitinase with an extended binding cleft.

Ohnuma T, Dozen S, Honda Y, Kitaoka M, Fukamizo T.

J Biochem. 2016 Aug;160(2):93-100. doi: 10.1093/jb/mvw014. Epub 2016 Feb 22.

PMID:
26908157
6.

Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.

Martínez-Caballero S, Cano-Sánchez P, Mares-Mejía I, Díaz-Sánchez AG, Macías-Rubalcava ML, Hermoso JA, Rodríguez-Romero A.

FEBS J. 2014 Oct;281(19):4535-54. doi: 10.1111/febs.12962. Epub 2014 Sep 6.

7.

The complete amino acid sequence of chitinase-a from the seeds of rye (Secale cereal).

Yamagami T, Funatsu G.

Biosci Biotechnol Biochem. 1994 Feb;58(2):322-9.

8.

Molecular cloning, functional expression, and mutagenesis of cDNA encoding rye (Secale cereale) seed chitinase-c.

Ohnuma T, Yagi M, Yamagami T, Taira T, Aso Y, Ishiguro M.

Biosci Biotechnol Biochem. 2002 Feb;66(2):277-84.

9.
10.

Localization, accumulation, and antifungal activity of chitinases in rye (Secale cereale) seed.

Taira T, Yamagami T, Aso Y, Ishiguro M, Ishihara M.

Biosci Biotechnol Biochem. 2001 Dec;65(12):2710-8.

11.

Chitin oligosaccharide binding to a family GH19 chitinase from the moss Bryum coronatum.

Ohnuma T, Sørlie M, Fukuda T, Kawamoto N, Taira T, Fukamizo T.

FEBS J. 2011 Nov;278(21):3991-4001. doi: 10.1111/j.1742-4658.2011.08301.x. Epub 2011 Sep 27.

12.

A glycosynthase derived from an inverting GH19 chitinase from the moss Bryum coronatum.

Ohnuma T, Fukuda T, Dozen S, Honda Y, Kitaoka M, Fukamizo T.

Biochem J. 2012 Jun 15;444(3):437-43. doi: 10.1042/BJ20120036.

PMID:
22435672
13.

Chitin oligosaccharide binding to the lysin motif of a novel type of chitinase from the multicellular green alga, Volvox carteri.

Kitaoku Y, Fukamizo T, Numata T, Ohnuma T.

Plant Mol Biol. 2017 Jan;93(1-2):97-108. doi: 10.1007/s11103-016-0549-5. Epub 2016 Nov 2.

PMID:
27807643
14.

Positions of disulfide bonds in rye (Secale cereale) seed chitinase-a.

Yamagami T, Funatsu G, Ishiguro M.

Biosci Biotechnol Biochem. 2000 Jun;64(6):1313-6.

15.

Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of `loopless' family GH19 chitinase from Bryum coronatum in a complex with chitotetraose.

Ohnuma T, Umemoto N, Taira T, Fukamizo T, Numata T.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1360-2. doi: 10.1107/S1744309113028935. Epub 2013 Nov 28.

16.

Chitinase-catalyzed hydrolysis of 4-nitrophenyl penta-N-acetyl-β-chitopentaoside as determined by real-time ESIMS: the 4-nitrophenyl moiety of the substrate interacts with the enzyme binding site.

Letzel T, Sahmel-Schneider E, Skriver K, Ohnuma T, Fukamizo T.

Carbohydr Res. 2011 May 1;346(6):863-6. doi: 10.1016/j.carres.2011.01.012. Epub 2011 Jan 18.

PMID:
21397215
17.

Interaction of di-N-acetylchitobiosyl moranoline with a family GH19 chitinase from moss, Bryum coronatum.

Shinya S, Urasaki A, Ohnuma T, Taira T, Suzuki A, Ogata M, Usui T, Lampela O, Juffer AH, Fukamizo T.

Glycobiology. 2014 Oct;24(10):945-55. doi: 10.1093/glycob/cwu052. Epub 2014 Jun 6.

PMID:
24907709
18.

Modulation of the transglycosylation activity of plant family GH18 chitinase by removing or introducing a tryptophan side chain.

Umemoto N, Ohnuma T, Osawa T, Numata T, Fukamizo T.

FEBS Lett. 2015 Aug 19;589(18):2327-33. doi: 10.1016/j.febslet.2015.07.018. Epub 2015 Jul 26.

19.

The complete amino acid sequence of chitinase-c from the seeds of rye (Secale cereal).

Yamagami T, Funatsu G.

Biosci Biotechnol Biochem. 1993 Nov;57(11):1854-61.

20.

Involvements of Trp23 in the Chitin-binding and of Trp131 in the Chitinase Activity of Rye Seed Chitinase-a.

Yamagami T, Funatsu G.

Biosci Biotechnol Biochem. 1997 Jan;61(11):1819-25. doi: 10.1271/bbb.61.1819.

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