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Items: 1 to 20 of 126

1.

An improved strategy for easy process monitoring and advanced purification of recombinant proteins.

Miladi B, Dridi C, El Marjou A, Boeuf G, Bouallagui H, Dufour F, Di Martino P, Elm'selmi A.

Mol Biotechnol. 2013 Nov;55(3):227-35. doi: 10.1007/s12033-013-9673-5.

PMID:
23780701
3.

One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag.

Keefe AD, Wilson DS, Seelig B, Szostak JW.

Protein Expr Purif. 2001 Dec;23(3):440-6.

PMID:
11722181
4.

Characterization of purified recombinant Bet v 1 with authentic N-terminus, cloned in fusion with maltose-binding protein.

Spangfort MD, Ipsen H, Sparholt SH, Aasmul-Olsen S, Larsen MR, Mørtz E, Roepstorff P, Larsen JN.

Protein Expr Purif. 1996 Nov;8(3):365-73.

PMID:
8936599
5.

Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli.

Austin BP, Nallamsetty S, Waugh DS.

Methods Mol Biol. 2009;498:157-72. doi: 10.1007/978-1-59745-196-3_11.

PMID:
18988025
6.

Orthogonal protein purification facilitated by a small bispecific affinity tag.

Nilvebrant J, Alm T, Hober S.

J Vis Exp. 2012 Jan 16;(59). pii: 3370. doi: 10.3791/3370.

7.

Expression and purification of soluble His(6)-tagged TEV protease.

Tropea JE, Cherry S, Waugh DS.

Methods Mol Biol. 2009;498:297-307. doi: 10.1007/978-1-59745-196-3_19.

PMID:
18988033
8.

Adsorptive detagging of poly-histidine tagged protein using hexa-histidine tagged exopeptidase.

Kuo WH, Chase HA.

J Chromatogr A. 2010 Dec 3;1217(49):7749-58. doi: 10.1016/j.chroma.2010.10.050. Epub 2010 Oct 21.

PMID:
21055759
9.

Single-step affinity and cost-effective purification of recombinant proteins using the Sepharose-binding lectin-tag from the mushroom Laetiporus sulphureus as fusion partner.

Li XJ, Liu JL, Gao DS, Wan WY, Yang X, Li YT, Chang HT, Chen L, Wang CQ, Zhao J.

Protein Expr Purif. 2016 Mar;119:51-6. doi: 10.1016/j.pep.2015.11.004. Epub 2015 Nov 23.

PMID:
26616099
10.

Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption.

Abdullah N, Chase HA.

Biotechnol Bioeng. 2005 Nov 20;92(4):501-13.

PMID:
16080185
11.

A modular set of prokaryotic and eukaryotic expression vectors.

Melcher K.

Anal Biochem. 2000 Jan 1;277(1):109-20. Erratum in: Anal Biochem 2000 Jul 1;282(2):266.

PMID:
10610695
13.
14.

Optimized soluble expression and purification of an aggregation-prone protein by fusion tag systems and on-column cleavage in Escherichia coli.

Li W, Gao M, Liu W, Kong Y, Tian H, Yao W, Gao X.

Protein Pept Lett. 2012 Dec;19(12):1324-9.

PMID:
22670677
15.

Esterase 2 from Alicyclobacillus acidocaldarius as a reporter and affinity tag for expression and single step purification of polypeptides.

Huang Y, Humenik M, Sprinzl M.

Protein Expr Purif. 2007 Jul;54(1):94-100. Epub 2007 Feb 17.

PMID:
17399994
16.

Purification of a secreted form of recombinant rabies virus glycoprotein: comparison of two affinity tags.

Wojczyk BS, Czerwinski M, Stwora-Wojczyk MM, Siegel DL, Abrams WR, Wunner WH, Spitalnik SL.

Protein Expr Purif. 1996 Mar;7(2):183-93.

PMID:
8812859
17.

Dual expression system suitable for high-throughput fluorescence-based screening and production of soluble proteins.

Braud S, Moutiez M, Belin P, Abello N, Drevet P, Zinn-Justin S, Courçon M, Masson C, Dassa J, Charbonnier JB, Boulain JC, Ménez A, Genet R, Gondry M.

J Proteome Res. 2005 Nov-Dec;4(6):2137-47.

PMID:
16335960
18.

A novel multi-affinity tag system to produce high levels of soluble and biotinylated proteins in Escherichia coli.

Ashraf SS, Benson RE, Payne ES, Halbleib CM, Grøn H.

Protein Expr Purif. 2004 Feb;33(2):238-45.

PMID:
14711512
20.

Expression and purification of recombinant proteins in Escherichia coli tagged with the metal-binding protein CusF.

Cantu-Bustos JE, Vargas-Cortez T, Morones-Ramirez JR, Balderas-Renteria I, Galbraith DW, McEvoy MM, Zarate X.

Protein Expr Purif. 2016 May;121:61-5. doi: 10.1016/j.pep.2016.01.007. Epub 2016 Jan 12.

PMID:
26805756

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