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Items: 1 to 20 of 74

1.

New approaches for sensing metabolites and proteins in live cells using RNA.

Strack RL, Jaffrey SR.

Curr Opin Chem Biol. 2013 Aug;17(4):651-5. doi: 10.1016/j.cbpa.2013.05.014. Epub 2013 Jun 6. Review.

2.

Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria.

Strack RL, Song W, Jaffrey SR.

Nat Protoc. 2014 Jan;9(1):146-55. doi: 10.1038/nprot.2014.001. Epub 2013 Dec 19.

3.

Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors.

Kellenberger CA, Hallberg ZF, Hammond MC.

Methods Mol Biol. 2015;1316:87-103. doi: 10.1007/978-1-4939-2730-2_8.

PMID:
25967055
4.

Tandem Spinach Array for mRNA Imaging in Living Bacterial Cells.

Zhang J, Fei J, Leslie BJ, Han KY, Kuhlman TE, Ha T.

Sci Rep. 2015 Nov 27;5:17295. doi: 10.1038/srep17295.

5.

Fluorescence imaging of cellular metabolites with RNA.

Paige JS, Nguyen-Duc T, Song W, Jaffrey SR.

Science. 2012 Mar 9;335(6073):1194. doi: 10.1126/science.1218298.

6.

Developing Fluorogenic Riboswitches for Imaging Metabolite Concentration Dynamics in Bacterial Cells.

Litke JL, You M, Jaffrey SR.

Methods Enzymol. 2016;572:315-33. doi: 10.1016/bs.mie.2016.03.021. Epub 2016 Apr 19.

7.

Understanding the photophysics of the spinach-DFHBI RNA aptamer-fluorogen complex to improve live-cell RNA imaging.

Han KY, Leslie BJ, Fei J, Zhang J, Ha T.

J Am Chem Soc. 2013 Dec 18;135(50):19033-8. doi: 10.1021/ja411060p. Epub 2013 Dec 10.

8.

Imaging and tracing of intracellular metabolites utilizing genetically encoded fluorescent biosensors.

Zhang C, Wei ZH, Ye BC.

Biotechnol J. 2013 Nov;8(11):1280-91. doi: 10.1002/biot.201300001. Epub 2013 Sep 25. Review.

PMID:
24591186
9.

FRET-based genetically-encoded sensors for quantitative monitoring of metabolites.

Mohsin M, Ahmad A, Iqbal M.

Biotechnol Lett. 2015 Oct;37(10):1919-28. doi: 10.1007/s10529-015-1873-6. Epub 2015 Jul 17. Review.

PMID:
26184603
10.

RNA-based fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP.

Kellenberger CA, Wilson SC, Sales-Lee J, Hammond MC.

J Am Chem Soc. 2013 Apr 3;135(13):4906-9. doi: 10.1021/ja311960g. Epub 2013 Mar 21.

11.

Using Spinach aptamer to correlate mRNA and protein levels in Escherichia coli.

Pothoulakis G, Ellis T.

Methods Enzymol. 2015;550:173-85. doi: 10.1016/bs.mie.2014.10.047. Epub 2015 Jan 5.

PMID:
25605386
12.

Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers.

Jeng SC, Chan HH, Booy EP, McKenna SA, Unrau PJ.

RNA. 2016 Dec;22(12):1884-1892. Epub 2016 Oct 24.

PMID:
27777365
13.

Broccoli: rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution.

Filonov GS, Moon JD, Svensen N, Jaffrey SR.

J Am Chem Soc. 2014 Nov 19;136(46):16299-308. doi: 10.1021/ja508478x. Epub 2014 Nov 5.

14.

Genetically-encoded FRET-based sensors for monitoring Zn(2+) in living cells.

Hessels AM, Merkx M.

Metallomics. 2015 Feb;7(2):258-66. doi: 10.1039/c4mt00179f. Review.

PMID:
25156481
15.

Live-cell imaging of mammalian RNAs with Spinach2.

Strack RL, Jaffrey SR.

Methods Enzymol. 2015;550:129-46. doi: 10.1016/bs.mie.2014.10.044. Epub 2015 Jan 6.

16.

Fluorescence resonance energy transfer imaging of cell signaling from in vitro to in vivo: basis of biosensor construction, live imaging, and image processing.

Aoki K, Kamioka Y, Matsuda M.

Dev Growth Differ. 2013 May;55(4):515-22. doi: 10.1111/dgd.12039. Epub 2013 Feb 7. Review.

PMID:
23387795
17.

An evaluation of genetically encoded FRET-based biosensors for quantitative metabolite analyses in vivo.

Moussa R, Baierl A, Steffen V, Kubitzki T, Wiechert W, Pohl M.

J Biotechnol. 2014 Dec 10;191:250-9. doi: 10.1016/j.jbiotec.2014.07.007. Epub 2014 Aug 5.

PMID:
25107505
18.

Rapid, randomized development of genetically encoded FRET sensors for small molecules.

Peroza EA, Boumezbeur AH, Zamboni N.

Analyst. 2015 Jul 7;140(13):4540-8. doi: 10.1039/c5an00707k.

PMID:
25988853
19.
20.

In vitro analysis of riboswitch-Spinach aptamer fusions as metabolite-sensing fluorescent biosensors.

Kellenberger CA, Hammond MC.

Methods Enzymol. 2015;550:147-72. doi: 10.1016/bs.mie.2014.10.045. Epub 2014 Dec 19.

PMID:
25605385

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