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Items: 1 to 20 of 102

1.

A simple, universal, efficient PCR-based gene synthesis method: sequential OE-PCR gene synthesis.

Zhang P, Ding Y, Liao W, Chen Q, Zhang H, Qi P, He T, Wang J, Deng S, Pan T, Ren H, Pan W.

Gene. 2013 Jul 25;524(2):347-54. doi: 10.1016/j.gene.2013.03.126. Epub 2013 Apr 15.

PMID:
23597923
2.

Improved PCR-based gene synthesis method and its application to the Citrobacter freundii phytase gene codon modification.

Gordeeva TL, Borschevskaya LN, Sineoky SP.

J Microbiol Methods. 2010 May;81(2):147-52. doi: 10.1016/j.mimet.2010.02.013. Epub 2010 Mar 10.

PMID:
20226218
3.

PCR-based accurate synthesis of long DNA sequences.

Xiong AS, Yao QH, Peng RH, Duan H, Li X, Fan HQ, Cheng ZM, Li Y.

Nat Protoc. 2006;1(2):791-7.

PMID:
17406309
4.

An improved method of gene synthesis based on DNA works software and overlap extension PCR.

Dong B, Mao R, Li B, Liu Q, Xu P, Li G.

Mol Biotechnol. 2007 Nov;37(3):195-200. Epub 2007 Aug 3.

PMID:
17952664
5.

A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR.

An Y, Ji J, Wu W, Lv A, Huang R, Wei Y.

Appl Microbiol Biotechnol. 2005 Oct;68(6):774-8. Epub 2005 Oct 13.

PMID:
16249879
6.

Two-step total gene synthesis method.

Young L, Dong Q.

Nucleic Acids Res. 2004 Apr 15;32(7):e59.

7.

Gene synthesis method based on overlap extension PCR and DNAWorks program.

Li G, Dong BX, Liu YH, Li CJ, Zhang LP.

Methods Mol Biol. 2013;1073:9-17. doi: 10.1007/978-1-62703-625-2_2.

PMID:
23996435
8.

Polymerase chain reaction-mediated gene synthesis: synthesis of a gene coding for isozyme c of horseradish peroxidase.

Jayaraman K, Fingar SA, Shah J, Fyles J.

Proc Natl Acad Sci U S A. 1991 May 15;88(10):4084-8.

9.

Synthetic gene for the hepatitis C virus nucleocapsid protein.

Khudyakov YE, Fields HA, Favorov MO, Khudyakova NS, Bonafonte MT, Holloway B.

Nucleic Acids Res. 1993 Jun 11;21(11):2747-54.

10.

Simplified gene synthesis: a one-step approach to PCR-based gene construction.

Wu G, Wolf JB, Ibrahim AF, Vadasz S, Gunasinghe M, Freeland SJ.

J Biotechnol. 2006 Jul 25;124(3):496-503. Epub 2006 Mar 3.

PMID:
16516321
11.

A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis.

Peng RH, Xiong AS, Yao QH.

Appl Microbiol Biotechnol. 2006 Nov;73(1):234-40. Epub 2006 Oct 5.

PMID:
17021880
12.

Integrated two-step gene synthesis in a microfluidic device.

Huang MC, Ye H, Kuan YK, Li MH, Ying JY.

Lab Chip. 2009 Jan 21;9(2):276-85. doi: 10.1039/b807688j. Epub 2008 Oct 23.

PMID:
19107285
13.

Synthesis of a gene for the HIV transactivator protein TAT by a novel single stranded approach involving in vivo gap repair.

Adams SE, Johnson ID, Braddock M, Kingsman AJ, Kingsman SM, Edwards RM.

Nucleic Acids Res. 1988 May 25;16(10):4287-98.

14.

Gene synthesis and expression in E. coli for pump, a human matrix metalloproteinase.

Ye QZ, Johnson LL, Baragi V.

Biochem Biophys Res Commun. 1992 Jul 15;186(1):143-9.

PMID:
1632763
15.
16.

Chemical-enzymatic synthesis, cloning and expression of a synthetic gene coding for an env protein fragment of the human T-cell leukemia virus type 1.

Rosenthal A, Ulrich R, Billwitz H, Frank R, Blöcker H.

Nucleic Acids Symp Ser. 1987;(18):233-6.

PMID:
2827130
17.

Quikgene: a gene synthesis method integrated with ligation-free cloning.

Mao Y, Lin J, Zhou A, Ji K, Downey JS, Chen R, Han A.

Anal Biochem. 2011 Aug 1;415(1):21-6. doi: 10.1016/j.ab.2011.04.004. Epub 2011 Apr 15.

PMID:
21530481
18.

The construction and use of bacterial DNA microarrays based on an optimized two-stage PCR strategy.

Postier BL, Wang HL, Singh A, Impson L, Andrews HL, Klahn J, Li H, Risinger G, Pesta D, Deyholos M, Galbraith DW, Sherman LA, Burnap RL.

BMC Genomics. 2003 Jun 12;4(1):23. Epub 2003 Jun 12.

19.

Production of a full length Tat protein in E. coli and its purification.

Armengaud J, de Nuova Perez L, Lemay P, Masson JM.

FEBS Lett. 1991 Apr 22;282(1):157-60.

20.

Total chemical synthesis, assembly of human torque teno virus genome.

Hou Z, Xiao G.

Virol Sin. 2011 Jun;26(3):181-9. doi: 10.1007/s12250-011-3187-8. Epub 2011 Jun 12.

PMID:
21667338

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