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Items: 1 to 20 of 116

1.

A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.

Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT.

BMC Biotechnol. 2013 Jan 3;13:1. doi: 10.1186/1472-6750-13-1.

2.

A toolkit and robust pipeline for the generation of fosmid-based reporter genes in C. elegans.

Tursun B, Cochella L, Carrera I, Hobert O.

PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 2009 Mar 4.

3.

Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host.

Westenberg M, Bamps S, Soedling H, Hope IA, Dolphin CT.

BMC Biotechnol. 2010 Mar 29;10:27. doi: 10.1186/1472-6750-10-27.

4.
5.

The production of C. elegans transgenes via recombineering with the galK selectable marker.

Zhang Y, Kashyap L, Ferguson AA, Fisher AL.

J Vis Exp. 2011 Jan 11;(47). pii: 2331. doi: 10.3791/2331.

6.

A simplified, robust, and streamlined procedure for the production of C. elegans transgenes via recombineering.

Zhang Y, Nash L, Fisher AL.

BMC Dev Biol. 2008 Dec 30;8:119. doi: 10.1186/1471-213X-8-119.

7.

Recombination-mediated genetic engineering of large genomic DNA transgenes.

Ejsmont RK, Ahlfeld P, Pozniakovsky A, Stewart AF, Tomancak P, Sarov M.

Methods Mol Biol. 2011;772:445-58. doi: 10.1007/978-1-61779-228-1_26.

PMID:
22065454
8.

Homologous recombination-mediated cloning and manipulation of genomic DNA regions using Gateway and recombineering systems.

Rozwadowski K, Yang W, Kagale S.

BMC Biotechnol. 2008 Nov 17;8:88. doi: 10.1186/1472-6750-8-88.

9.

Counter-selection recombineering of the baculovirus genome: a strategy for seamless modification of repeat-containing BACs.

Westenberg M, Soedling HM, Mann DA, Nicholson LJ, Dolphin CT.

Nucleic Acids Res. 2010 Sep;38(16):e166. doi: 10.1093/nar/gkq596. Epub 2010 Jul 9.

10.

Genetic manipulation of the Toxoplasma gondii genome by fosmid recombineering.

Vinayak S, Brooks CF, Naumov A, Suvorova ES, White MW, Striepen B.

MBio. 2014 Dec 2;5(6):e02021. doi: 10.1128/mBio.02021-14.

11.
12.

Efficient generation of long-distance conditional alleles using recombineering and a dual selection strategy in replicate plates.

Voehringer D, Wu D, Liang HE, Locksley RM.

BMC Biotechnol. 2009 Jul 28;9:69. doi: 10.1186/1472-6750-9-69.

13.

Production of fosmid genomic libraries optimized for liquid culture recombineering and cross-species transgenesis.

Ejsmont RK, Bogdanzaliewa M, Lipinski KA, Tomancak P.

Methods Mol Biol. 2011;772:423-43. doi: 10.1007/978-1-61779-228-1_25.

PMID:
22065453
14.

Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette.

Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B.

Genetics. 2015 Aug;200(4):1035-49. doi: 10.1534/genetics.115.178335. Epub 2015 Jun 3.

15.

The Caenorhabditis elegans sirtuin gene, sir-2.1, is widely expressed and induced upon caloric restriction.

Bamps S, Wirtz J, Savory FR, Lake D, Hope IA.

Mech Ageing Dev. 2009 Nov-Dec;130(11-12):762-70. doi: 10.1016/j.mad.2009.10.001.

PMID:
19896965
16.
17.

A recombineering pipeline for functional genomics applied to Caenorhabditis elegans.

Sarov M, Schneider S, Pozniakovski A, Roguev A, Ernst S, Zhang Y, Hyman AA, Stewart AF.

Nat Methods. 2006 Oct;3(10):839-44.

PMID:
16990816
18.

Engineering EGFP reporter constructs into a 200 kb human beta-globin BAC clone using GET Recombination.

Orford M, Nefedov M, Vadolas J, Zaibak F, Williamson R, Ioannou PA.

Nucleic Acids Res. 2000 Sep 15;28(18):E84.

19.

Construction and evaluation of a new triple-gene expression cassette vaccinia virus shuttle vector.

Du S, Li C, Wang Y, Liu C, Ren D, Li Y, Qin Y, Wang M, Sun D, Zhu N, Jin N.

J Virol Methods. 2012 Nov;185(2):175-83. doi: 10.1016/j.jviromet.2012.06.022. Epub 2012 Jul 2.

PMID:
22766182
20.

Expression analysis of ABC transporters reveals differential functions of tandemly duplicated genes in Caenorhabditis elegans.

Zhao Z, Sheps JA, Ling V, Fang LL, Baillie DL.

J Mol Biol. 2004 Nov 19;344(2):409-17.

PMID:
15522294

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