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Items: 1 to 20 of 122

1.

Errors in ribosomal sequence datasets generated using PCR-coupled 'panbacterial' pyrosequencing, and the establishment of an improved approach.

Prosdocimi EM, Novati S, Bruno R, Bandi C, Mulatto P, Giannico R, Casiraghi M, Ferri E.

Mol Cell Probes. 2013 Feb;27(1):65-7. doi: 10.1016/j.mcp.2012.07.003. Epub 2012 Jul 20.

PMID:
22824825
2.

Capturing greater 16S rRNA gene sequence diversity within the domain Bacteria.

Winsley T, van Dorst JM, Brown MV, Ferrari BC.

Appl Environ Microbiol. 2012 Aug;78(16):5938-41. doi: 10.1128/AEM.01299-12. Epub 2012 Jun 8.

3.

Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments.

O'Sullivan LA, Webster G, Fry JC, Parkes RJ, Weightman AJ.

J Microbiol Methods. 2008 Dec;75(3):579-81. doi: 10.1016/j.mimet.2008.08.006. Epub 2008 Aug 26.

PMID:
18789360
4.

Review and re-analysis of domain-specific 16S primers.

Baker GC, Smith JJ, Cowan DA.

J Microbiol Methods. 2003 Dec;55(3):541-55. Review.

PMID:
14607398
5.

Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting.

Watanabe K, Kodama Y, Harayama S.

J Microbiol Methods. 2001 Apr;44(3):253-62.

PMID:
11240048
6.

Coamplification of eukaryotic DNA with 16S rRNA gene-based PCR primers: possible consequences for population fingerprinting of complex microbial communities.

Huys G, Vanhoutte T, Joossens M, Mahious AS, De Brandt E, Vermeire S, Swings J.

Curr Microbiol. 2008 Jun;56(6):553-7. doi: 10.1007/s00284-008-9122-z. Epub 2008 Feb 27.

PMID:
18301945
7.
8.

Multiple group-specific sequencing primers for reliable and rapid DNA sequencing.

Gharizadeh B, Ohlin A, Mölling P, Bäckman A, Amini B, Olcén P, Nyrén P.

Mol Cell Probes. 2003 Aug;17(4):203-10.

PMID:
12944124
9.

Scratching the surface of the rare biosphere with ribosomal sequence tag primers.

Neufeld JD, Li J, Mohn WW.

FEMS Microbiol Lett. 2008 Jun;283(2):146-53. doi: 10.1111/j.1574-6968.2008.01124.x. Epub 2008 Apr 21.

10.

Cross-kingdom amplification using bacteria-specific primers: complications for studies of coral microbial ecology.

Galkiewicz JP, Kellogg CA.

Appl Environ Microbiol. 2008 Dec;74(24):7828-31. doi: 10.1128/AEM.01303-08. Epub 2008 Oct 17.

11.

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets.

Pinto AJ, Raskin L.

PLoS One. 2012;7(8):e43093. doi: 10.1371/journal.pone.0043093. Epub 2012 Aug 15.

12.

Phylogenetic identification of uncultured pathogens using ribosomal RNA sequences.

Schmidt TM, Relman DA.

Methods Enzymol. 1994;235:205-22. Review. No abstract available.

PMID:
7520119
13.

Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA.

Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, Dymock D, Wade WG.

Appl Environ Microbiol. 1998 Feb;64(2):795-9. Erratum in: Appl Environ Microbiol 1998 Jun;64(6):2333.

14.

Improved group-specific PCR primers for denaturing gradient gel electrophoresis analysis of the genetic diversity of complex microbial communities.

Mühling M, Woolven-Allen J, Murrell JC, Joint I.

ISME J. 2008 Apr;2(4):379-92. doi: 10.1038/ismej.2007.97. Epub 2008 Mar 13.

PMID:
18340335
15.

High-density universal 16S rRNA microarray analysis reveals broader diversity than typical clone library when sampling the environment.

DeSantis TZ, Brodie EL, Moberg JP, Zubieta IX, Piceno YM, Andersen GL.

Microb Ecol. 2007 Apr;53(3):371-83. Epub 2007 Mar 2.

PMID:
17334858
16.

Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method.

Wu JY, Jiang XT, Jiang YX, Lu SY, Zou F, Zhou HW.

BMC Microbiol. 2010 Oct 12;10:255. doi: 10.1186/1471-2180-10-255.

17.

Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies.

Schloss PD, Gevers D, Westcott SL.

PLoS One. 2011;6(12):e27310. doi: 10.1371/journal.pone.0027310. Epub 2011 Dec 14.

18.

Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification.

Zucol F, Ammann RA, Berger C, Aebi C, Altwegg M, Niggli FK, Nadal D.

J Clin Microbiol. 2006 Aug;44(8):2750-9.

19.

Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes.

Frank JA, Reich CI, Sharma S, Weisbaum JS, Wilson BA, Olsen GJ.

Appl Environ Microbiol. 2008 Apr;74(8):2461-70. doi: 10.1128/AEM.02272-07. Epub 2008 Feb 22.

20.

PCR-based community structure studies of bacteria associated with eukaryotic organisms: a simple PCR strategy to avoid co-amplification of eukaryotic DNA.

Bakke I, De Schryver P, Boon N, Vadstein O.

J Microbiol Methods. 2011 Feb;84(2):349-51. doi: 10.1016/j.mimet.2010.12.015. Epub 2010 Dec 21.

PMID:
21182876
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