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Items: 1 to 20 of 250

1.

Probing the GTPase cycle with real-time NMR: GAP and GEF activities in cell extracts.

Marshall CB, Meiri D, Smith MJ, Mazhab-Jafari MT, Gasmi-Seabrook GM, Rottapel R, Stambolic V, Ikura M.

Methods. 2012 Aug;57(4):473-85. doi: 10.1016/j.ymeth.2012.06.014.

PMID:
22750304
2.
3.

Regulation of small GTPases by GEFs, GAPs, and GDIs.

Cherfils J, Zeghouf M.

Physiol Rev. 2013 Jan;93(1):269-309. doi: 10.1152/physrev.00003.2012. Review.

4.

Biochemical assays to characterize Rho GTPases.

Jaiswal M, Dubey BN, Koessmeier KT, Gremer L, Ahmadian MR.

Methods Mol Biol. 2012;827:37-58. doi: 10.1007/978-1-61779-442-1_3.

PMID:
22144266
5.

GEFs and GAPs: critical elements in the control of small G proteins.

Bos JL, Rehmann H, Wittinghofer A.

Cell. 2007 Jun 1;129(5):865-77. Review. Erratum in: Cell. 2007 Jul 27;130(2):385.

6.

In vitro GEF and GAP assays.

Eberth A, Ahmadian MR.

Curr Protoc Cell Biol. 2009 Jun;Chapter 14:Unit 14.9. doi: 10.1002/0471143030.cb1409s43.

PMID:
19499504
7.

Structural basis for the unique biological function of small GTPase RHEB.

Yu Y, Li S, Xu X, Li Y, Guan K, Arnold E, Ding J.

J Biol Chem. 2005 Apr 29;280(17):17093-100.

8.

Real-time NMR study of three small GTPases reveals that fluorescent 2'(3')-O-(N-methylanthraniloyl)-tagged nucleotides alter hydrolysis and exchange kinetics.

Mazhab-Jafari MT, Marshall CB, Smith M, Gasmi-Seabrook GM, Stambolic V, Rottapel R, Neel BG, Ikura M.

J Biol Chem. 2010 Feb 19;285(8):5132-6. doi: 10.1074/jbc.C109.064766.

9.
10.

Investigation of the GTP-binding/GTPase cycle of Cdc42Hs using extrinsic reporter group fluorescence.

Nomanbhoy TK, Leonard DA, Manor D, Cerione RA.

Biochemistry. 1996 Apr 9;35(14):4602-8.

PMID:
8605211
11.
12.

Gbetagamma inhibits Galpha GTPase-activating proteins by inhibition of Galpha-GTP binding during stimulation by receptor.

Tang W, Tu Y, Nayak SK, Woodson J, Jehl M, Ross EM.

J Biol Chem. 2006 Feb 24;281(8):4746-53.

13.
14.

Detection of Rho GEF and GAP activity through a sensitive split luciferase assay system.

Anderson EL, Hamann MJ.

Biochem J. 2012 Feb 1;441(3):869-79. doi: 10.1042/BJ20111111. Erratum in: Biochem J. 2012 Jun 15;444(3):619.

PMID:
22004470
15.

Mechanism of the guanine nucleotide exchange reaction of Ras GTPase--evidence for a GTP/GDP displacement model.

Zhang B, Zhang Y, Shacter E, Zheng Y.

Biochemistry. 2005 Feb 22;44(7):2566-76.

PMID:
15709769
16.

Regulatory proteins of R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3.

Ohba Y, Mochizuki N, Yamashita S, Chan AM, Schrader JW, Hattori S, Nagashima K, Matsuda M.

J Biol Chem. 2000 Jun 30;275(26):20020-6.

17.

A homogeneous fluorescence resonance energy transfer system for monitoring the activation of a protein switch in real time.

Bill A, Blockus H, Stumpfe D, Bajorath J, Schmitz A, Famulok M.

J Am Chem Soc. 2011 Jun 1;133(21):8372-9. doi: 10.1021/ja202513s.

PMID:
21517092
20.

GTP hydrolysis mechanisms in ras p21 and in the ras-GAP complex studied by fluorescence measurements on tryptophan mutants.

Antonny B, Chardin P, Roux M, Chabre M.

Biochemistry. 1991 Aug 27;30(34):8287-95.

PMID:
1883817
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