Sort by
Items per page

Send to

Choose Destination

Links from PubMed

Items: 1 to 20 of 149


Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities.

Zylicz-Stachula A, Zolnierkiewicz O, Lubys A, Ramanauskaite D, Mitkaite G, Bujnicki JM, Skowron PM.

BMC Mol Biol. 2012 Apr 10;13:13. doi: 10.1186/1471-2199-13-13.


Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus.

Skowron PM, Vitkute J, Ramanauskaite D, Mitkaite G, Jezewska-Frackowiak J, Zebrowska J, Zylicz-Stachula A, Lubys A.

BMC Mol Biol. 2013 Aug 6;14:17. doi: 10.1186/1471-2199-14-17.


Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving.

Zylicz-Stachula A, Zołnierkiewicz O, Sliwińska K, Jeżewska-Frąckowiak J, Skowron PM.

BMC Biochem. 2011 Dec 5;12:62. doi: 10.1186/1471-2091-12-62.


A new prototype IIS/IIC/IIG endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus, recognising 5'-TARCCA(N11/9)-3' sequences.

Jezewska-Frackowiak J, Lubys A, Vitkute J, Zakareviciene L, Zebrowska J, Krefft D, Skowron MA, Zylicz-Stachula A, Skowron PM.

J Biotechnol. 2015 Jan 20;194:19-26. doi: 10.1016/j.jbiotec.2014.11.023. Epub 2014 Dec 4.


Two-stage gene assembly/cloning of a member of the TspDTI subfamily of bifunctional restriction endonucleases, TthHB27I.

Krefft D, Zylicz-Stachula A, Mulkiewicz E, Papkov A, Jezewska-Frackowiak J, Skowron PM.

J Biotechnol. 2015 Jan 20;194:67-80. doi: 10.1016/j.jbiotec.2014.11.030. Epub 2014 Dec 6.


Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family.

Zylicz-Stachula A, Bujnicki JM, Skowron PM.

BMC Mol Biol. 2009 May 29;10:52. doi: 10.1186/1471-2199-10-52. Erratum in: BMC Mol Biol. 2014;15:16.


Modified 'one amino acid-one codon' engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase.

Zylicz-Stachula A, Zolnierkiewicz O, Sliwinska K, Jezewska-Frackowiak J, Skowron PM.

Microb Cell Fact. 2014 Jan 11;13:7. doi: 10.1186/1475-2859-13-7.


A new Thermus sp. class-IIS enzyme sub-family: isolation of a 'twin' endonuclease TspDTI with a novel specificity 5'-ATGAA(N(11/9))-3', related to TspGWI, TaqII and Tth111II.

Skowron PM, Majewski J, Zylicz-Stachula A, Rutkowska SM, Jaworowska I, Harasimowicz-Słowińska RI.

Nucleic Acids Res. 2003 Jul 15;31(14):e74.


Cofactor analogue-induced chemical reactivation of endonuclease activity in a DNA cleavage/methylation deficient TspGWI N₄₇₃A variant in the NPPY motif.

Zylicz-Stachula A, Jeżewska-Frąckowiak J, Skowron PM.

Mol Biol Rep. 2014;41(4):2313-23. doi: 10.1007/s11033-014-3085-x. Epub 2014 Jan 19.


A putative Type IIS restriction endonuclease GeoICI from Geobacillus sp.--A robust, thermostable alternative to mezophilic prototype BbvI.

Zebrowska J, Zolnierkiewicz O, Skowron MA, Zylicz-Stachula A, Jezewska-Frackowiak J, Skowron PM.

J Biosci. 2016 Mar;41(1):27-38.


Type II restriction endonuclease R.Hpy188I belongs to the GIY-YIG nuclease superfamily, but exhibits an unusual active site.

Kaminska KH, Kawai M, Boniecki M, Kobayashi I, Bujnicki JM.

BMC Struct Biol. 2008 Nov 14;8:48. doi: 10.1186/1472-6807-8-48.


A new genomic tool, ultra-frequently cleaving TaqII/sinefungin endonuclease with a combined 2.9-bp recognition site, applied to the construction of horse DNA libraries.

Zylicz-Stachula A, Zolnierkiewicz O, Jasiecki J, Skowron PM.

BMC Genomics. 2013 Jun 1;14:370. doi: 10.1186/1471-2164-14-370.


A homology model of restriction endonuclease SfiI in complex with DNA.

Chmiel AA, Bujnicki JM, Skowronek KJ.

BMC Struct Biol. 2005 Jan 24;5:2.


The MmeI family: type II restriction-modification enzymes that employ single-strand modification for host protection.

Morgan RD, Dwinell EA, Bhatia TK, Lang EM, Luyten YA.

Nucleic Acids Res. 2009 Aug;37(15):5208-21. doi: 10.1093/nar/gkp534. Epub 2009 Jul 3.


The third restriction-modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities.

Skowron PM, Anton BP, Czajkowska E, Zebrowska J, Sulecka E, Krefft D, Jezewska-Frackowiak J, Zolnierkiewicz O, Witkowska M, Morgan RD, Wilson GG, Fomenkov A, Roberts RJ, Zylicz-Stachula A.

Nucleic Acids Res. 2017 Sep 6;45(15):9005-9018. doi: 10.1093/nar/gkx599.


Chemically-induced affinity star restriction specificity: a novel TspGWI/sinefungin endonuclease with theoretical 3-bp cleavage frequency.

Zylicz-Stachula A, Zołnierkiewicz O, Jeżewska-Frąckowiak J, Skowron PM.

Biotechniques. 2011 Jun;50(6):397-406. doi: 10.2144/000113685.


Characterization of cleavage intermediate and star sites of RM.Tth111II.

Zhu Z, Guan S, Robinson D, El Fezzazi H, Quimby A, Xu SY.

Sci Rep. 2014 Jan 23;4:3838. doi: 10.1038/srep03838.

Supplemental Content

Support Center