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Defined xenogeneic-free and hypoxic environment provides superior conditions for long-term expansion of human adipose-derived stem cells.

Yang S, Pilgaard L, Chase LG, Boucher S, Vemuri MC, Fink T, Zachar V.

Tissue Eng Part C Methods. 2012 Aug;18(8):593-602. doi: 10.1089/ten.TEC.2011.0592. Epub 2012 Apr 3.


Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells.

Patrikoski M, Juntunen M, Boucher S, Campbell A, Vemuri MC, Mannerström B, Miettinen S.

Stem Cell Res Ther. 2013 Mar 7;4(2):27. doi: 10.1186/scrt175.


Comparative Analysis of Media and Supplements on Initiation and Expansion of Adipose-Derived Stem Cells.

Riis S, Nielsen FM, Pennisi CP, Zachar V, Fink T.

Stem Cells Transl Med. 2016 Mar;5(3):314-24. doi: 10.5966/sctm.2015-0148. Epub 2016 Feb 2.


Accelerated and safe proliferation of human adipose-derived stem cells in medium supplemented with human serum.

Josh F, Kobe K, Tobita M, Tanaka R, Suzuki K, Ono K, Hyakusoku H, Mizuno H.

J Nippon Med Sch. 2012;79(6):444-52.


A defined and xeno-free culture method enabling the establishment of clinical-grade human embryonic, induced pluripotent and adipose stem cells.

Rajala K, Lindroos B, Hussein SM, Lappalainen RS, Pekkanen-Mattila M, Inzunza J, Rozell B, Miettinen S, Narkilahti S, Kerkelä E, Aalto-Setälä K, Otonkoski T, Suuronen R, Hovatta O, Skottman H.

PLoS One. 2010 Apr 19;5(4):e10246. doi: 10.1371/journal.pone.0010246.


Development and characterization of a clinically compliant xeno-free culture medium in good manufacturing practice for human multipotent mesenchymal stem cells.

Chase LG, Yang S, Zachar V, Yang Z, Lakshmipathy U, Bradford J, Boucher SE, Vemuri MC.

Stem Cells Transl Med. 2012 Oct;1(10):750-8. doi: 10.5966/sctm.2012-0072. Epub 2012 Oct 10.


Xeno-Free Extraction, Culture, and Cryopreservation of Human Adipose-Derived Mesenchymal Stem Cells.

Escobar CH, Chaparro O.

Stem Cells Transl Med. 2016 Mar;5(3):358-65. doi: 10.5966/sctm.2015-0094. Epub 2016 Feb 2.


Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation.

Paula AC, Martins TM, Zonari A, Frade SP, Angelo PC, Gomes DA, Goes AM.

Stem Cell Res Ther. 2015 Apr 14;6:76. doi: 10.1186/s13287-015-0030-4.


Isolation and expansion of adipose-derived stem cells for tissue engineering.

Fink T, Rasmussen JG, Lund P, Pilgaard L, Soballe K, Zachar V.

Front Biosci (Elite Ed). 2011 Jan 1;3:256-63.


Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived products.

Li Y, Powell S, Brunette E, Lebkowski J, Mandalam R.

Biotechnol Bioeng. 2005 Sep 20;91(6):688-98.


Toward a clinical-grade expansion of mesenchymal stem cells from human sources: a microcarrier-based culture system under xeno-free conditions.

Santos Fd, Andrade PZ, Abecasis MM, Gimble JM, Chase LG, Campbell AM, Boucher S, Vemuri MC, Silva CL, Cabral JM.

Tissue Eng Part C Methods. 2011 Dec;17(12):1201-10. doi: 10.1089/ten.tec.2011.0255. Epub 2011 Sep 6.


Adipose tissue mesenchymal stem cell expansion in animal serum-free medium supplemented with autologous human platelet lysate.

Blande IS, Bassaneze V, Lavini-Ramos C, Fae KC, Kalil J, Miyakawa AA, Schettert IT, Krieger JE.

Transfusion. 2009 Dec;49(12):2680-5. doi: 10.1111/j.1537-2995.2009.02346.x. Epub 2009 Aug 18.


Effect of growth media and serum replacements on the proliferation and differentiation of adipose-derived stem cells.

Lund P, Pilgaard L, Duroux M, Fink T, Zachar V.

Cytotherapy. 2009;11(2):189-97. doi: 10.1080/14653240902736266.


Optimization of culture conditions for the expansion of umbilical cord-derived mesenchymal stem or stromal cell-like cells using xeno-free culture conditions.

Hatlapatka T, Moretti P, Lavrentieva A, Hass R, Marquardt N, Jacobs R, Kasper C.

Tissue Eng Part C Methods. 2011 Apr;17(4):485-93. doi: 10.1089/ten.TEC.2010.0406. Epub 2011 Jan 19.


Human mesenchymal stem cells from the umbilical cord matrix: successful isolation and ex vivo expansion using serum-/xeno-free culture media.

Simões IN, Boura JS, dos Santos F, Andrade PZ, Cardoso CM, Gimble JM, da Silva CL, Cabral JM.

Biotechnol J. 2013 Apr;8(4):448-58. doi: 10.1002/biot.201200340. Epub 2013 Mar 7.


A xeno-free microcarrier-based stirred culture system for the scalable expansion of human mesenchymal stem/stromal cells isolated from bone marrow and adipose tissue.

Carmelo JG, Fernandes-Platzgummer A, Diogo MM, da Silva CL, Cabral JM.

Biotechnol J. 2015 Aug;10(8):1235-47. doi: 10.1002/biot.201400586. Epub 2015 Jul 24.


Mass spectrometry analysis of adipose-derived stem cells reveals a significant effect of hypoxia on pathways regulating extracellular matrix.

Riis S, Stensballe A, Emmersen J, Pennisi CP, Birkelund S, Zachar V, Fink T.

Stem Cell Res Ther. 2016 Apr 14;7(1):52. doi: 10.1186/s13287-016-0310-7.


An update on niche composition, signaling and functional regulation of the adipose-derived stem cells.

Kim WS, Han J, Hwang SJ, Sung JH.

Expert Opin Biol Ther. 2014 Aug;14(8):1091-102. doi: 10.1517/14712598.2014.907785. Epub 2014 Apr 8. Review.


Optimization and scale-up culture of human endometrial multipotent mesenchymal stromal cells: potential for clinical application.

Rajaraman G, White J, Tan KS, Ulrich D, Rosamilia A, Werkmeister J, Gargett CE.

Tissue Eng Part C Methods. 2013 Jan;19(1):80-92. doi: 10.1089/ten.TEC.2011.0718. Epub 2012 Aug 2.


The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering.

Pytlík R, Stehlík D, Soukup T, Kalbácová M, Rypácek F, Trc T, Mulinková K, Michnová P, Kideryová L, Zivný J, Klener P Jr, Veselá R, Trnený M, Klener P.

Biomaterials. 2009 Jul;30(20):3415-27. doi: 10.1016/j.biomaterials.2009.03.001. Epub 2009 Apr 10.


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